Bioinformatics and Genetic Analysis of Micropropagation To Preserve the Endemic and Almost Extinct Cibotium Barometz

Abstract

In vitro culture is very important for the development of increasingly rare Simpei fern (Cibotium Barometz) cultivars. The use of nitrogen can affect the sustainability of breeding traits. This study aims to further investigate the effect of giving variations in nitrogen concentration (KNO3) to the genetic diversity of the Cibotium barometz plantet produced from in vitro culture. Buds were collected and cultured on Murashige and Skoog (MS) medium with different nitrogen concentrations (KNO3) for in vitro culture formation. In this study, RAPD analysis was applied to identify 4 Cibotium Barometz planlets. Five RAPD primers were prepared for this analysis. Our results indicate that the DNA band pattern polymorphism produced from 5 RAPD primers shows very high diversity up to 100%. The results of the analysis of the RAPD band pattern clustering using the UPGMA method on the similarity coefficient of 0.68 and the analysis of the main components were able to be clearly distinguished into 3 groups. Genetic diversity that occurs indicates that nitrogen can cause negligence in Cibotium Barometz. The use of RAPD-derived DNA markers to authenticate Cibotium barometz from in vitro culture can help clarify the genetic diversity that occurs.