Study of Antioxidant Activity, Peptides, and Chemical Quality of Goat Milk Kefir on the Different Post-Acidification Periods During Cold Storage

A. K. Umam, L. Radiati, Kevin Hutomo Putra Suwondo, S. Kholidah
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Abstract

The interaction between raw goat milk, lactic acid bacteria (LAB), and kefir grain resulted in the unique taste and flavor of fermented products. Under optimum temperature, LAB and yeast had the better condition for growth and optimizing metabolic activity. The proportion level of kefir grains should be considered during the incubation period because it could change the logarithmic growth phase of lactic acid bacteria. After the processing, cold storage should be applied to suppress microorganism growth in the post-acidification period. The prolonged post-acidification period potentially affects the quality of Goat milk kefir products. This research aimed to evaluate the effect of a post-acidification period during cold storage based on antioxidant, peptides, and chemical quality of goat milk kefir. The experimental design was a completely randomized design (RCD) with four treatments and three replications. The quality analysis was done in the different post-acidification periods for 1 day, 7 days, 14 days, and 21 days after being stored at 4C. Duncan Multiple Range Test (DMRT) was used for further statistical analysis. The results showed that the different postacidification periods had significant effects (p<0.05) on antioxidant activity, peptide, fat, and ethanol content. On Average, antioxidant activity, peptide, fat, and ethanol content in goat-milk kefir were 55.70 ± 2.25 %, 9.32 ± 0.13 mg / ml, 1.86 ± 0.08%, and 1.04 ± 0.04%, respectively. In conclusion, the longer period of post-acidification has a major contribution to the change of goat milk kefir characteristics during cold storage.
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羊乳开菲尔冷藏后不同酸化期抗氧化活性、多肽及化学品质的研究
生羊奶与乳酸菌、开菲尔颗粒相互作用,形成独特的风味。在最适温度下,乳酸菌和酵母具有较好的生长条件和优化代谢活性。在乳酸菌发酵过程中,应考虑开菲尔颗粒的比例水平,因为它会改变乳酸菌的对数生长阶段。加工后应采用冷藏,以抑制后酸化期微生物的生长。酸化后时间过长可能会影响羊乳开菲尔产品的质量。本研究旨在评价羊奶开菲尔冷藏后酸化期对其抗氧化、多肽和化学品质的影响。试验设计为完全随机设计(RCD), 4个处理,3个重复。在4℃保存后1天、7天、14天和21天的不同酸化期进行质量分析。采用Duncan多元极差检验(DMRT)进行进一步统计分析。结果表明,不同酸化期对鱼的抗氧化活性、多肽、脂肪和乙醇含量均有显著影响(p<0.05)。羊乳开菲尔的抗氧化活性、肽、脂肪和乙醇含量平均分别为55.70±2.25%、9.32±0.13 mg / ml、1.86±0.08%和1.04±0.04%。综上所述,较长的后酸化时间对羊乳开菲尔冷藏期间特性的变化有重要影响。
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