PHYTOCHEMICAL ANALYSIS AND ANTIOBESITY PROPERTIES OF ENDEMIC Jurinea brevicaulis Boiss.

Abstract

Obesity, defined as new world syndrome, is an epidemic that causes global health problems and big economic losses. Natural products have been gain increasing importance for the epidemic because of their antiobesity potency. Jurinea genus comprised approximately 200 species worldwide and has been used as a therapeutic agent for colic, fever, gout, and rheumatism, traditionally. The aim of this study was to analyze the volatile components, to determine its phenolic compounds and to evaluate its antiobesity potentcy of Jurinea brevicaulis BOISS. A widespread method based on solid-phase microextraction technique (SPME) was employed for GC-MS analysis of volatile components of J. brevicaulis. Quantitative analysis of phenolic compounds of J. brevicaulis was performed using RP-HPLC. To evaluate the antiobesity effect, the inhibitory effects of α-amylase, α-glucosidase, and lipase of the species were determined. A total of 19 volatile components were specified and o-cymene (10.60 %), β-Bisabolene (9.30 %), and sesquicineole (57.5 %) for different terpenes were described as major components. According to HPLC analysis, sinapic acid, p-coumaric acid, and quercetin were determined for the species. IC50 value of the species was determined as 36.59 ± 2.37 μg/mL and 42.56 ± 2.83 μg/mL for α-amylase and α-glucosidase assays, respectively. IC50 value was found 50.31 ± 3.75 μg/mL, according to lipase inhibition studies. Considering all the findings, it has been determined that the species is included diverse volatile components, antiobesity effective three phenolic compounds, and showed promising antiobesity potential so J. brevicaulis may be the up-and-coming candidate of natural product for obesity.