Pub Date : 2023-10-27DOI: 10.23902/trkjnat.1339397
Merve Yılmazer, Semian Karaer Uzuner
Glycogen synthase kinase 3β is a serine/threonine kinase that functions in numerous cellular processes such as cell proliferation, DNA repair, cell cycle, signaling, and metabolic pathways. GSK3β plays a role in several diseases, including inflammation, neurodegenerative disease, diabetes, and cancer. Yeasts are suitable models for the investigation of various cellular processes because they include homologous genes to human genes. In this study, we transferred the human GSK3β gene to Schizosaccharomyces pombe cells (gsk3Δ), including a deletion for this gene. Cells with gsk3 gene deletion and transformant cells with the human GSK3β gene that was reversed by genetic complementation were comparatively examined at the level of gene expression for changes in cellular respiration under varying glucose concentration conditions. For this purpose, the expression of fbp1, pka1 and gsk3 genes were analyzed in cells grown under conditions containing different glucose concentrations. We concluded that the GSK3β gene was expressed more in glucose starvation than in other conditions. We also observed a decrease in the level of gene expression in mitochondrial respiration when the human GSK3β gene was transferred in cells that preferred mitochondrial respiration in the absence of the gsk3 gene, regardless of ambient conditions.
{"title":"Effects of Glucose on the Cellular Respiration in Fission Yeast Expressing Human GSK3β Gene","authors":"Merve Yılmazer, Semian Karaer Uzuner","doi":"10.23902/trkjnat.1339397","DOIUrl":"https://doi.org/10.23902/trkjnat.1339397","url":null,"abstract":"Glycogen synthase kinase 3β is a serine/threonine kinase that functions in numerous cellular processes such as cell proliferation, DNA repair, cell cycle, signaling, and metabolic pathways. GSK3β plays a role in several diseases, including inflammation, neurodegenerative disease, diabetes, and cancer. Yeasts are suitable models for the investigation of various cellular processes because they include homologous genes to human genes. In this study, we transferred the human GSK3β gene to Schizosaccharomyces pombe cells (gsk3Δ), including a deletion for this gene. Cells with gsk3 gene deletion and transformant cells with the human GSK3β gene that was reversed by genetic complementation were comparatively examined at the level of gene expression for changes in cellular respiration under varying glucose concentration conditions. For this purpose, the expression of fbp1, pka1 and gsk3 genes were analyzed in cells grown under conditions containing different glucose concentrations. We concluded that the GSK3β gene was expressed more in glucose starvation than in other conditions. We also observed a decrease in the level of gene expression in mitochondrial respiration when the human GSK3β gene was transferred in cells that preferred mitochondrial respiration in the absence of the gsk3 gene, regardless of ambient conditions.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"227 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1288588
Esin POYRAZOĞLU, Mediha ONUR
In this study, the isolation and identification of bacteria that form biofilms on various food products sold on open-air market stalls in Aydın Province were aimed. Bacteria were isolated from food surfaces in aseptic conditions and isolated after incubation in the Tryptic Soy Agar medium at 37°C for 24-48 hours. DNA isolations of the isolated bacteria were performed and the PCR products obtained were used for sequencing. The Congo Red Agar method was used to qualitatively analyze biofilm formation. According to this method, bacteria forming black colonies were evaluated as biofilm-positive and were subjected to quantitative analysis using the Microplate method. A total of 67 bacteria were isolated from the sampled food items, of which 7 were strong and 2 were moderate biofilm producers, showing that more importance should be given to food hygiene.
{"title":"ISOLATION AND IDENTIFICATION OF BIOFILM-FORMING BACTERIA IN THE FOODS SUPPLIED FOR CONSUMPTION AT THE SALES STALLS IN THE PROVINCE OF AYDIN","authors":"Esin POYRAZOĞLU, Mediha ONUR","doi":"10.23902/trkjnat.1288588","DOIUrl":"https://doi.org/10.23902/trkjnat.1288588","url":null,"abstract":"In this study, the isolation and identification of bacteria that form biofilms on various food products sold on open-air market stalls in Aydın Province were aimed. Bacteria were isolated from food surfaces in aseptic conditions and isolated after incubation in the Tryptic Soy Agar medium at 37°C for 24-48 hours. DNA isolations of the isolated bacteria were performed and the PCR products obtained were used for sequencing. The Congo Red Agar method was used to qualitatively analyze biofilm formation. According to this method, bacteria forming black colonies were evaluated as biofilm-positive and were subjected to quantitative analysis using the Microplate method. A total of 67 bacteria were isolated from the sampled food items, of which 7 were strong and 2 were moderate biofilm producers, showing that more importance should be given to food hygiene.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"20 10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1324202
Metin Burak TATLISES, Semra HASANCEBİ
Substitution of plant cultivars of high commercial value with a cheaper, lower quality one is a common fraud committed against consumers and producers. Since it is one of the most widely grown legumes, lentil (Lens culinaris Medik.) is suitable for such frauds. This study aimed to identify lentil cultivars which are registered and authorized in the market in Türkiye by using current molecular methods. For this purpose, 26 lentil cultivars were analyzed for 15 SSR markers and two DNA barcode regions (trnH-psbA and matK). A high allele diversity was observed by 12 scorable SSR markers, and the average number of alleles was determined to be 16. One of the important findings was the presence of “cultivar-specific alleles” that can be used to identify each cultivar in the lentil market in Türkiye. At least one “cultivar-specific allele” was obtained for each cultivar. The lentil cultivars were also analyzed by two DNA barcode regions as trnH-psbA and matK. While it was observed that the rate of the intra-species variation for the trnH-psbA region was low and 26 varieties were divided into 7 groups, higher rate was found for matK and samples were distributed into 14 groups. Nevertheless, it was observed that intra-species discrimination can be made more effective when both loci are used together and 26 species were distributed into 18 different groups. We expect that the results of this study, especially the cultivar-specific SSR alleles and DNA barcoding sequence data may be used routinely to identify production and packaged products that are commercially available in markets.
{"title":"IDENTIFICATION OF LENS CULTIVARS IN MARKET BY MOLECULAR TOOLS: DNA BARCODING AND SSRs","authors":"Metin Burak TATLISES, Semra HASANCEBİ","doi":"10.23902/trkjnat.1324202","DOIUrl":"https://doi.org/10.23902/trkjnat.1324202","url":null,"abstract":"Substitution of plant cultivars of high commercial value with a cheaper, lower quality one is a common fraud committed against consumers and producers. Since it is one of the most widely grown legumes, lentil (Lens culinaris Medik.) is suitable for such frauds. This study aimed to identify lentil cultivars which are registered and authorized in the market in Türkiye by using current molecular methods. For this purpose, 26 lentil cultivars were analyzed for 15 SSR markers and two DNA barcode regions (trnH-psbA and matK). A high allele diversity was observed by 12 scorable SSR markers, and the average number of alleles was determined to be 16. One of the important findings was the presence of “cultivar-specific alleles” that can be used to identify each cultivar in the lentil market in Türkiye. At least one “cultivar-specific allele” was obtained for each cultivar. The lentil cultivars were also analyzed by two DNA barcode regions as trnH-psbA and matK. While it was observed that the rate of the intra-species variation for the trnH-psbA region was low and 26 varieties were divided into 7 groups, higher rate was found for matK and samples were distributed into 14 groups. Nevertheless, it was observed that intra-species discrimination can be made more effective when both loci are used together and 26 species were distributed into 18 different groups. We expect that the results of this study, especially the cultivar-specific SSR alleles and DNA barcoding sequence data may be used routinely to identify production and packaged products that are commercially available in markets.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136185137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1331537
Ilgaz AKATA, İsmail ŞEN, Ergin SAHİN, Bekir ÇÖL, Emre KESKİN
In the present study, fungal samples collected from Enez and Süloğlu districts of Edirne Province, in Türkiye with the help of truffle-detecting dogs were analyzed. The macroscopic features and environmental details of the collection sites were documented in their natural habitats. The samples were investigated with light- and scanning electron microscopy (SEM) and were also used in ITS rDNA-based molecular phylogenetic analysis, which revealed that they belong to Delastria rosea Tul. & C. Tul. The identification was further supported by morphological data. This is the first record of D. rosea in Türkiye at the genus and species level. A brief description of the newly reported species is provided. Macro- and microphotographs of the spores taken by both ligth and electron microscop (SEM). With this study, the number of genera and species of Turkish truffles and truffle-like fungi has increased to 36 and 105, respectively.
{"title":"Delastria TUL. & C. TUL., A NEW TRUFFLE GENUS RECORD FOR TURKISH MYCOBIOTA","authors":"Ilgaz AKATA, İsmail ŞEN, Ergin SAHİN, Bekir ÇÖL, Emre KESKİN","doi":"10.23902/trkjnat.1331537","DOIUrl":"https://doi.org/10.23902/trkjnat.1331537","url":null,"abstract":"In the present study, fungal samples collected from Enez and Süloğlu districts of Edirne Province, in Türkiye with the help of truffle-detecting dogs were analyzed. The macroscopic features and environmental details of the collection sites were documented in their natural habitats. The samples were investigated with light- and scanning electron microscopy (SEM) and were also used in ITS rDNA-based molecular phylogenetic analysis, which revealed that they belong to Delastria rosea Tul. & C. Tul. The identification was further supported by morphological data. This is the first record of D. rosea in Türkiye at the genus and species level. A brief description of the newly reported species is provided. Macro- and microphotographs of the spores taken by both ligth and electron microscop (SEM). With this study, the number of genera and species of Turkish truffles and truffle-like fungi has increased to 36 and 105, respectively.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1309119
Gizem DEYNEZ, Ece MİSER SALİHOĞLU, İpek SÜNTAR
Peritoneal adhesion occurs as a result of surgery, peritoneal injury, peritonitis, hypoxia, and ischemia. Surgical trauma causes many pathophysiological processes which include inflammation, oxidation, coagulation, fibrinolysis, cell proliferation, and apoptosis. After intra-abdominal operations, the adhesion tissue may occur on the peritoneal surface due to low fibrinolytic activity. This may result in permanent excessive adhesion tissue bands instead of properly formed fibrin structures. Therefore, anticoagulant, thrombolytic, and fibrinolytic activities have a key role in preventing peritoneal adhesion. Indeed, several studies have been conducted to find out new and effective agents against intra-abdominal adhesion. Thus, revealing the causes, development processes, and investigation techniques are highly important for designing and conducting such scientific studies. In this context, this study aims to summarize the pathophysiological processes of above-mentioned activities and to emphasize their importance in the peritoneal adhesion model as well as to explain the evaluation methods, particularly in terms of the investigation of natural products.
{"title":"THE ROLE OF ANTICOAGULANT, THROMBOLYTIC, AND FIBRINOLYTIC ACTIVITIES IN THE PREVENTION OF PERITONEAL ADHESION","authors":"Gizem DEYNEZ, Ece MİSER SALİHOĞLU, İpek SÜNTAR","doi":"10.23902/trkjnat.1309119","DOIUrl":"https://doi.org/10.23902/trkjnat.1309119","url":null,"abstract":"Peritoneal adhesion occurs as a result of surgery, peritoneal injury, peritonitis, hypoxia, and ischemia. Surgical trauma causes many pathophysiological processes which include inflammation, oxidation, coagulation, fibrinolysis, cell proliferation, and apoptosis. After intra-abdominal operations, the adhesion tissue may occur on the peritoneal surface due to low fibrinolytic activity. This may result in permanent excessive adhesion tissue bands instead of properly formed fibrin structures. Therefore, anticoagulant, thrombolytic, and fibrinolytic activities have a key role in preventing peritoneal adhesion. Indeed, several studies have been conducted to find out new and effective agents against intra-abdominal adhesion. Thus, revealing the causes, development processes, and investigation techniques are highly important for designing and conducting such scientific studies. In this context, this study aims to summarize the pathophysiological processes of above-mentioned activities and to emphasize their importance in the peritoneal adhesion model as well as to explain the evaluation methods, particularly in terms of the investigation of natural products.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1302555
Çağla ERSÖZ, Deniz ALTUN ÇOLAK
In human body, which is exposed to number of chemicals and physical agents in daily life, malfunctions may occur from time to time in detoxification mechanisms that will prevent the harmful effects of genotoxic agents, whose negative effects cannot be eliminated. In this study, the genoprotective effects of bitter melon (Momordica charantia L.) seed and fruit extracts against the genotoxic potential of the antineoplastic agent cisplatin and the antiepileptic drug valproic acid in 72±4 hours old transheterozygous larvae of the fruit fly Drosophila melanogaster Meigen were determined by the Somatic Mutation and Recombination Test (SMART). The results showed that the mutation frequencies approached the negative control values by inhibiting the formation of chemical agent-induced mutant clones in all doses in the experimental groups in which plant extracts were applied at doses of 1.25, 2.5 ve 5 mg/mL, while the mutation frequency increased only in the groups that applied cisplatin and valproic acid. These results show that co-administration of cisplatin and valproic acid reduces the genotoxic effect of M. charantia.
{"title":"EFFECT OF BITTER MELON AGAINST CISPLATIN AND VALPROIC ACID-INDUCED GENOTOXICITY IN Drosophila melanogaster Meigen","authors":"Çağla ERSÖZ, Deniz ALTUN ÇOLAK","doi":"10.23902/trkjnat.1302555","DOIUrl":"https://doi.org/10.23902/trkjnat.1302555","url":null,"abstract":"In human body, which is exposed to number of chemicals and physical agents in daily life, malfunctions may occur from time to time in detoxification mechanisms that will prevent the harmful effects of genotoxic agents, whose negative effects cannot be eliminated. In this study, the genoprotective effects of bitter melon (Momordica charantia L.) seed and fruit extracts against the genotoxic potential of the antineoplastic agent cisplatin and the antiepileptic drug valproic acid in 72±4 hours old transheterozygous larvae of the fruit fly Drosophila melanogaster Meigen were determined by the Somatic Mutation and Recombination Test (SMART). The results showed that the mutation frequencies approached the negative control values by inhibiting the formation of chemical agent-induced mutant clones in all doses in the experimental groups in which plant extracts were applied at doses of 1.25, 2.5 ve 5 mg/mL, while the mutation frequency increased only in the groups that applied cisplatin and valproic acid. These results show that co-administration of cisplatin and valproic acid reduces the genotoxic effect of M. charantia.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1368563
Zafer KOÇAK
Research and scientific papers are measured, compared, and frequently ranked using journal metrics. They may also be referred to as journal rankings, journal relevance, or journal impact. Journal metrics allow academics and researchers to compare scholarly publications. The most prestigious ones are the Journal Impact Factor (JIF) based on Web of Science data, and CiteScore and SCImago Journal Rank (SJR) based on Scopus data. As we all know, the availability of these metrics for a journal is associated with its indexing in these bibliographic databases, such as the Web of Science Core Collection or Scopus, and is a proxy to determining the quality of the journal.
The number of open access journals is growing rapidly. According to a report by the University of Regensburg Library, there were more than 60,000 open access journals worldwide in 2018 (EBZ 2018). While open access has brought many benefits to academic publishing, it has also introduced us to many low-quality (Non-indexed) journals, as well as predatory journals and publishers. Thousands of journals therefore compete for manuscripts from researchers/authors. It is clear how difficult it is for new and low-quality journals to receive manuscripts in this competitive environment. Naturally, authors do not want to submit their research to journals that are not indexed or have no metrics. Recently, in some countries, publishing in predatory journals has even become a barrier to academic promotion (Koçak 2012).
研究和科学论文被衡量、比较，并经常使用期刊指标进行排名。它们也可以被称为期刊排名、期刊相关性或期刊影响力。期刊指标允许学者和研究人员比较学术出版物。最负盛名的是基于Web of Science数据的期刊影响因子(JIF)，以及基于Scopus数据的CiteScore和SCImago期刊排名(SJR)。我们都知道，期刊的这些指标的可用性与其在这些书目数据库中的索引有关，例如Web of Science Core Collection或Scopus，并且是确定期刊质量的代理。& # x0D;开放获取期刊的数量正在迅速增长。根据雷根斯堡大学图书馆的一份报告，2018年全球有超过6万种开放获取期刊(EBZ 2018)。虽然开放获取给学术出版带来了很多好处，但它也向我们介绍了许多低质量(无索引)期刊，以及掠夺性期刊和出版商。因此，成千上万的期刊竞相获得研究人员/作者的手稿。很明显，在这种竞争激烈的环境下，低质量的新期刊获得稿件是多么困难。自然，作者不希望将他们的研究提交给没有索引或没有指标的期刊。最近，在一些国家，在掠夺性期刊上发表文章甚至成为学术推广的障碍(koak 2012)。
{"title":"Misleading metrics: predatory trade expands","authors":"Zafer KOÇAK","doi":"10.23902/trkjnat.1368563","DOIUrl":"https://doi.org/10.23902/trkjnat.1368563","url":null,"abstract":"Research and scientific papers are measured, compared, and frequently ranked using journal metrics. They may also be referred to as journal rankings, journal relevance, or journal impact. Journal metrics allow academics and researchers to compare scholarly publications. The most prestigious ones are the Journal Impact Factor (JIF) based on Web of Science data, and CiteScore and SCImago Journal Rank (SJR) based on Scopus data. As we all know, the availability of these metrics for a journal is associated with its indexing in these bibliographic databases, such as the Web of Science Core Collection or Scopus, and is a proxy to determining the quality of the journal. 
 The number of open access journals is growing rapidly. According to a report by the University of Regensburg Library, there were more than 60,000 open access journals worldwide in 2018 (EBZ 2018). While open access has brought many benefits to academic publishing, it has also introduced us to many low-quality (Non-indexed) journals, as well as predatory journals and publishers. Thousands of journals therefore compete for manuscripts from researchers/authors. It is clear how difficult it is for new and low-quality journals to receive manuscripts in this competitive environment. Naturally, authors do not want to submit their research to journals that are not indexed or have no metrics. Recently, in some countries, publishing in predatory journals has even become a barrier to academic promotion (Koçak 2012).","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"3 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1324756
Nurgül KARLIOĞLU KILIÇ, Ergün BACAK, Rüya YILMAZ DAĞDEVİREN, Arif Cemal ÖZSEMİR, Elif Ayşe YILDIRIM, Umut GÜNGÖR, Vedat BEŞKARDEŞ
During pollination, pollen grains can be transported to distant distances by various factors such as wind, insects, birds, and reptiles. While there have been numerous studies on pollen transportation by birds in Europe, there is a lack of research concerning the pollen grains transported by bird species in Turkey. In this context, we explored the role of Sylviidae species in pollination and identified the plant species whose pollen is transported by these birds. The temporary ringing station located at İstanbul University-Cerrahpaşa Avcılar Campus was selected as the study area and a total of 35 feather samples were collected from individuals of Sylvia atricapilla (Linnaeus), S. borin (Boddaert), Curruca communis (Latham), C. cantillans (Pallas), C. curruca (Linnaeus) and C. melanocephala (Gmelin) captured at station. Based on the analysis of pollen found on the feather samples, it was determined that the pollen found in 18 feather samples of Curruca curruca and Sylvia atricapilla provided pollen belonging to Pinus (Pine), Aesculus (Horse chestnut), Prunus (Plum), Spiraea (Spiraea), Quercus (Oak), Fraxinus (Ash) and Melia (Bead tree). The most commonly determined pollen on C. curruca were of Prunus (63.5%), while the least common were of Aesculus (0.9%). The most commonly determined pollen on S. atricapilla were of Melia (48.1%), while the least common were of Crocus (0.2%). The presence of pollen among the food sources of Sylviidae species during spring, the synchronization between the arrival time of birds in the study area during migration and the pollination period of the determined plant taxa, and the prevalence of these taxa in the study area suggest that S. atricapilla and C. curruca may be contributing to pollination.
{"title":"AN EVALUATION OF SYLVIIDAE SPECIES IN THE TRANSPORT OF POLLEN: A CASE STUDY OF İSTANBUL","authors":"Nurgül KARLIOĞLU KILIÇ, Ergün BACAK, Rüya YILMAZ DAĞDEVİREN, Arif Cemal ÖZSEMİR, Elif Ayşe YILDIRIM, Umut GÜNGÖR, Vedat BEŞKARDEŞ","doi":"10.23902/trkjnat.1324756","DOIUrl":"https://doi.org/10.23902/trkjnat.1324756","url":null,"abstract":"During pollination, pollen grains can be transported to distant distances by various factors such as wind, insects, birds, and reptiles. While there have been numerous studies on pollen transportation by birds in Europe, there is a lack of research concerning the pollen grains transported by bird species in Turkey. In this context, we explored the role of Sylviidae species in pollination and identified the plant species whose pollen is transported by these birds. The temporary ringing station located at İstanbul University-Cerrahpaşa Avcılar Campus was selected as the study area and a total of 35 feather samples were collected from individuals of Sylvia atricapilla (Linnaeus), S. borin (Boddaert), Curruca communis (Latham), C. cantillans (Pallas), C. curruca (Linnaeus) and C. melanocephala (Gmelin) captured at station. Based on the analysis of pollen found on the feather samples, it was determined that the pollen found in 18 feather samples of Curruca curruca and Sylvia atricapilla provided pollen belonging to Pinus (Pine), Aesculus (Horse chestnut), Prunus (Plum), Spiraea (Spiraea), Quercus (Oak), Fraxinus (Ash) and Melia (Bead tree). The most commonly determined pollen on C. curruca were of Prunus (63.5%), while the least common were of Aesculus (0.9%). The most commonly determined pollen on S. atricapilla were of Melia (48.1%), while the least common were of Crocus (0.2%). The presence of pollen among the food sources of Sylviidae species during spring, the synchronization between the arrival time of birds in the study area during migration and the pollination period of the determined plant taxa, and the prevalence of these taxa in the study area suggest that S. atricapilla and C. curruca may be contributing to pollination.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1247186
Ayşe ÜSTÜN, Ayşenur YAZICI, Serkan ÖRTUCU
In this study the extracellular proteins from the isolate LC3 belonging to Aspergillus were purified for new antimicrobial polypeptide (AMP) discovery and then tested for antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Methicillin-resistant S. aureus (MRSA). Antimicrobial activity was determined by the trypsin/proteinase K assay, which was polypeptide-based, and it was observed that this protein was a protein of about 11 kDa by gel overlay assay. The minimum inhibitory concentration of purified AMP molecule against S. aureus ATCC 25923 and MRSA was 8 µg/ml and 32 µg/ml, respectively and the AMP molecule was confirmed. ITS sequence analysis showed that isolate LC3 was identified as Aspergillus niger, using the Bioedit sequence assembly program. The sequence was deposited with the GenBank database with accession number MK332597. The results indicate that the purified AMP molecule has the potential to be used in infections caused by S. aureus.
{"title":"ISOLATION OF A NOVEL ANTIMICROBIAL POLYPEPTIDE FROM AN Aspergillus niger ISOLATE","authors":"Ayşe ÜSTÜN, Ayşenur YAZICI, Serkan ÖRTUCU","doi":"10.23902/trkjnat.1247186","DOIUrl":"https://doi.org/10.23902/trkjnat.1247186","url":null,"abstract":"In this study the extracellular proteins from the isolate LC3 belonging to Aspergillus were purified for new antimicrobial polypeptide (AMP) discovery and then tested for antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Methicillin-resistant S. aureus (MRSA). Antimicrobial activity was determined by the trypsin/proteinase K assay, which was polypeptide-based, and it was observed that this protein was a protein of about 11 kDa by gel overlay assay. The minimum inhibitory concentration of purified AMP molecule against S. aureus ATCC 25923 and MRSA was 8 µg/ml and 32 µg/ml, respectively and the AMP molecule was confirmed. ITS sequence analysis showed that isolate LC3 was identified as Aspergillus niger, using the Bioedit sequence assembly program. The sequence was deposited with the GenBank database with accession number MK332597. The results indicate that the purified AMP molecule has the potential to be used in infections caused by S. aureus.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"13 7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The main aim of this study is antimicrobial screening of the n-hexane, ethyl acetate and aqueous extracts obtained from aerial parts of Achillea setacea Waldst. & Kit. and Achillea coarctata Poir. against the bacterial strains Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 14028 and the yeast Candida albicans ATCC 6633 by the microdilution method. In addition, the effect of the aqueous extract of A. coarctata combined with fluconazole againts C. albicans ATCC 6633, C. parapsilosis ATCC 22019, C. tropicalis ATCC 750 and C. glabrata ATCC 90030 was tested with the checkerboard method. The chemical components of the volatiles and fatty acid methyl esters were identified using the Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS) techniques. The main components of the volatile oil were found to be 1,8-cineole (16.1%) and β-pinene (14.5%) for A. setacea and camphor (35.4%) and borneol (12.9%) for A. coarctata. The ethyl acetate extracts of A. setacea (MIC=156.25 µg/mL) and A. coarctata (MIC=312.5 µg/mL) were found more active against the tested Candida Berkh. species. The combination of aqueous extract of A. coarctata with fluconazole showed additive effect for the tested Candida species with FIC values within the range of 0.53-0.625 µg/mL.
{"title":"Screening of two Achillea (Asteraceae) species from Turkey, Achillea coarctata Poir. and Achillea setacea Waldst. & Kit. for volatiles and fatty acids compositions, and antimicrobial activity","authors":"Gamze GÖGER, Esma ÇEVİK, Alişan VARNALI, Ömer Koray YAYLACI, Medine Münevver UMA, Gülmira ÖZEK","doi":"10.23902/trkjnat.1322140","DOIUrl":"https://doi.org/10.23902/trkjnat.1322140","url":null,"abstract":"The main aim of this study is antimicrobial screening of the n-hexane, ethyl acetate and aqueous extracts obtained from aerial parts of Achillea setacea Waldst. & Kit. and Achillea coarctata Poir. against the bacterial strains Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 14028 and the yeast Candida albicans ATCC 6633 by the microdilution method. In addition, the effect of the aqueous extract of A. coarctata combined with fluconazole againts C. albicans ATCC 6633, C. parapsilosis ATCC 22019, C. tropicalis ATCC 750 and C. glabrata ATCC 90030 was tested with the checkerboard method. The chemical components of the volatiles and fatty acid methyl esters were identified using the Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS) techniques. The main components of the volatile oil were found to be 1,8-cineole (16.1%) and β-pinene (14.5%) for A. setacea and camphor (35.4%) and borneol (12.9%) for A. coarctata. The ethyl acetate extracts of A. setacea (MIC=156.25 µg/mL) and A. coarctata (MIC=312.5 µg/mL) were found more active against the tested Candida Berkh. species. The combination of aqueous extract of A. coarctata with fluconazole showed additive effect for the tested Candida species with FIC values within the range of 0.53-0.625 µg/mL.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":"279 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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