Pub Date : 2024-03-15DOI: 10.23902/trkjnat.1410800
Irfan Cinar, M. Yayla, Erdem Toktay, Damla Binnetoğlu
Liver injury from paracetamol (acetaminophen) (APAP) is common worldwide. To prevent intoxication with a drug with high poisoning, treatment can be made possible with an easily accessible and harmless substance. This study aimed to investigate the hepatoprotective ef-fects of Gossypin (GOS) in mice exposed to an overdose of APAP -the possible mechanism of action. Specifically, serum [alanine aminotransferase (ALT), aspartate transaminase (AST), and hepatic biochemical parameters (glutathione (GSH), malondialdehyde (MDA) and super-oxide dismutase (SOD)] were evaluated. Protein and mRNA levels of inflammatory, apoptot-ic, and cytochrome factors, including TNF-α, IL-1β, IL-6, NF-kB, and CYP2E1, were ana-lyzed using real-time PCR. Pretreatment with GOS significantly reduced APAP-induced he-patic injury via oxidative stress. Along with potent antioxidant activity, GOS promoted APAP hepatic detoxification by regulating AST, ALT, GSH, MDA, and SOD activities and mRNA levels of the cytochrome CYP2E1 gene. The anti-inflammatory activity of GOS in-creases its production. TNF-α, IL-1β and IL-6, through possible NF-kB blockade, are also responsible for its hepatoprotective effect. Taken together, GOS has the potential to be devel-oped as a preventive agent to be administered to patients suffering from APAP overdose.
{"title":"Effects of gossypin on acetaminophen-induced hepatotoxicity in mice","authors":"Irfan Cinar, M. Yayla, Erdem Toktay, Damla Binnetoğlu","doi":"10.23902/trkjnat.1410800","DOIUrl":"https://doi.org/10.23902/trkjnat.1410800","url":null,"abstract":"Liver injury from paracetamol (acetaminophen) (APAP) is common worldwide. To prevent intoxication with a drug with high poisoning, treatment can be made possible with an easily accessible and harmless substance. This study aimed to investigate the hepatoprotective ef-fects of Gossypin (GOS) in mice exposed to an overdose of APAP -the possible mechanism of action. Specifically, serum [alanine aminotransferase (ALT), aspartate transaminase (AST), and hepatic biochemical parameters (glutathione (GSH), malondialdehyde (MDA) and super-oxide dismutase (SOD)] were evaluated. Protein and mRNA levels of inflammatory, apoptot-ic, and cytochrome factors, including TNF-α, IL-1β, IL-6, NF-kB, and CYP2E1, were ana-lyzed using real-time PCR. Pretreatment with GOS significantly reduced APAP-induced he-patic injury via oxidative stress. Along with potent antioxidant activity, GOS promoted APAP hepatic detoxification by regulating AST, ALT, GSH, MDA, and SOD activities and mRNA levels of the cytochrome CYP2E1 gene. The anti-inflammatory activity of GOS in-creases its production. TNF-α, IL-1β and IL-6, through possible NF-kB blockade, are also responsible for its hepatoprotective effect. Taken together, GOS has the potential to be devel-oped as a preventive agent to be administered to patients suffering from APAP overdose.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140391599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-21DOI: 10.23902/trkjnat.1356270
Ebru Uzunhisarcıklı
Lung cancer is a type of cancer that is mostly diagnosed at an advanced stage and has a short survival time despite standard chemotherapy and targeted therapies. Terbutaline and Orciprenaline are bronchodilator agent that are potent and selective β2 receptor agonists. The purpose of this study was to investigate by continuous monitoring to assay the effects of Terbutaline and Orciprenaline on human lung carcinoma cell line. Cells were treated 1, 10, 100, 200, 400 µM concentrations with Terbutaline/Orciprenaline. MTT assay and xCELLigence real-time cell analyzer were used to determine these compounds effect on cell viability. The cell index was monitored continuously by visualizing the impedance of the E-plate wells. Because of GSK3β roles in a diverse range of cellular processes like metabolism, cell proliferation, differentiation and survival and its key position at several signaling pathways, GSK3β inhibition was investigated of these molecules. It has been determined that Terbutaline and Orciprenaline inhibits GSK-3β. This is the first known study to demonstrate the effects of Terbutaline and Orciprenaline on A549 and the human bronchial epithelial cell line Beas-2b in real time. In this study, it was detected that Terbutaline and especially Orciprenaline may have potential therapeutic effects in lung carcinoma.
{"title":"In vitro EVALUATION of THE EFFECTS of POTENTIAL GSK-3β INHIBITORS TERBUTALINE and ORCIPRENALINE","authors":"Ebru Uzunhisarcıklı","doi":"10.23902/trkjnat.1356270","DOIUrl":"https://doi.org/10.23902/trkjnat.1356270","url":null,"abstract":"Lung cancer is a type of cancer that is mostly diagnosed at an advanced stage and has a short survival time despite standard chemotherapy and targeted therapies. Terbutaline and Orciprenaline are bronchodilator agent that are potent and selective β2 receptor agonists. The purpose of this study was to investigate by continuous monitoring to assay the effects of Terbutaline and Orciprenaline on human lung carcinoma cell line. Cells were treated 1, 10, 100, 200, 400 µM concentrations with Terbutaline/Orciprenaline. MTT assay and xCELLigence real-time cell analyzer were used to determine these compounds effect on cell viability. The cell index was monitored continuously by visualizing the impedance of the E-plate wells. Because of GSK3β roles in a diverse range of cellular processes like metabolism, cell proliferation, differentiation and survival and its key position at several signaling pathways, GSK3β inhibition was investigated of these molecules. It has been determined that Terbutaline and Orciprenaline inhibits GSK-3β. \u0000This is the first known study to demonstrate the effects of Terbutaline and Orciprenaline on A549 and the human bronchial epithelial cell line Beas-2b in real time. In this study, it was detected that Terbutaline and especially Orciprenaline may have potential therapeutic effects in lung carcinoma.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140442898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-29DOI: 10.23902/trkjnat.1398909
Y. Aydın, Banu Orta Yilmaz, M. Erkan
Polychlorinated biphenyls (PCBs) are industrial substances which were widely used in industrial applications starting from the 1930s until the mid-1970s. Aroclor 1242 (A1242) is a commercial PCB mixture with 42% chlorine manufactured by the Monsanto Chemical Company in St. Louis, Missouri, USA. Previous studies suggested that PCBs have inhibitory effect on reproductive function, developmental abnormality, and impaired reproductive ability. PCBs may also affect the endocrine system by reducing the testosterone synthesis and the activity of steroidogenic enzymes in Leydig cells. This study was performed to investigate the specific effects of A1242 on the viability of Leydig cells, oxidative damage, and the profile of steroidogenic enzymes in an in vitro culture. The therapeutic effects of vitamin E (VitE) and genistein (Gen), as two antioxidants, in mitigating the damage produced by A1242 were also evaluated. TM3 Leydig cells were exposed to 10-8 and 10-6 M of A1242 and VitE (50µM) and Gen (10µM) as antioxidant for 24 h. After the exposure period, the Leydig cells were assessed to determine their viability using a cell viability assay. Measurements were performed for lipid peroxidation, reactive oxygen species (ROS), and steroidogenic enzymes. The results showed that cell viability was reduced after A1242 exposure, while lipid peroxidation and ROS increased. Steroidogenesis was interrupted in a concentration-dependent manner. Following A1242 exposure, administrations of VitE or Gen as an antioxidant reduced hazardous effects of A1242 on Leydig cells. Our results showed that exposure to A1242 may impair Leydig cell function and cause toxicity in Leydig cells and that VitE and Gen treatment exhibited therapeutic effects against this toxicity.
{"title":"Vitamin E and genistein generate a cytoprotective effect on polychlorinated biphenyl- induced oxidative stress in testicular Leydig cells","authors":"Y. Aydın, Banu Orta Yilmaz, M. Erkan","doi":"10.23902/trkjnat.1398909","DOIUrl":"https://doi.org/10.23902/trkjnat.1398909","url":null,"abstract":"Polychlorinated biphenyls (PCBs) are industrial substances which were widely used in industrial applications starting from the 1930s until the mid-1970s. Aroclor 1242 (A1242) is a commercial PCB mixture with 42% chlorine manufactured by the Monsanto Chemical Company in St. Louis, Missouri, USA. Previous studies suggested that PCBs have inhibitory effect on reproductive function, developmental abnormality, and impaired reproductive ability. PCBs may also affect the endocrine system by reducing the testosterone synthesis and the activity of steroidogenic enzymes in Leydig cells. This study was performed to investigate the specific effects of A1242 on the viability of Leydig cells, oxidative damage, and the profile of steroidogenic enzymes in an in vitro culture. The therapeutic effects of vitamin E (VitE) and genistein (Gen), as two antioxidants, in mitigating the damage produced by A1242 were also evaluated. TM3 Leydig cells were exposed to 10-8 and 10-6 M of A1242 and VitE (50µM) and Gen (10µM) as antioxidant for 24 h. After the exposure period, the Leydig cells were assessed to determine their viability using a cell viability assay. Measurements were performed for lipid peroxidation, reactive oxygen species (ROS), and steroidogenic enzymes. The results showed that cell viability was reduced after A1242 exposure, while lipid peroxidation and ROS increased. Steroidogenesis was interrupted in a concentration-dependent manner. Following A1242 exposure, administrations of VitE or Gen as an antioxidant reduced hazardous effects of A1242 on Leydig cells. Our results showed that exposure to A1242 may impair Leydig cell function and cause toxicity in Leydig cells and that VitE and Gen treatment exhibited therapeutic effects against this toxicity.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140488369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-17DOI: 10.23902/trkjnat.1373797
Burcu Yüksel, Asuman Deveci Özkan
Earthworm extracts have become an interesting research concept in cancer studies because they have various cancer-stopping and preventive substances. Determining the anticancer effect of the extract on cancer cells may lead to new treatment strategies and alternative medicine against the toxic effects of chemotherapeutic agents. Therefore, this study aims to raise awareness for drug development and future anticancer therapeutics by emphasizing the nature of earthworm extracts and the types of cancer they are used in through a systematic review. A systematic search of CINAHL, PubMed, Medline, Scopus, and Web of Science databases were conducted to identify articles published between January 2018 and December 2022. We utilized various arrangements of the keywords ‘earthworm’, ‘earthworm extract’, and ‘anticancer effect’. We identified original studies written in English depicting in vitro and in vivo cytotoxic impact of worm extracts on cancer cells were included in the review. We found 15 studies matching our search criteria. Among the studies, Eisenia Foetida was determined as the most studied type of earthworm, while Colon cancer was the most frequently studied cancer type. Coleomic Fluid was found to be the commonly used extract to test for anticancer activity with positive results. The study contributes to the literature by providing information about earthworm extracts' antiproliferative and antitumor effects.
{"title":"A systematic review of antiproliferative and antitumour activity of earthworm extracts","authors":"Burcu Yüksel, Asuman Deveci Özkan","doi":"10.23902/trkjnat.1373797","DOIUrl":"https://doi.org/10.23902/trkjnat.1373797","url":null,"abstract":"Earthworm extracts have become an interesting research concept in cancer studies because they have various cancer-stopping and preventive substances. Determining the anticancer effect of the extract on cancer cells may lead to new treatment strategies and alternative medicine against the toxic effects of chemotherapeutic agents. Therefore, this study aims to raise awareness for drug development and future anticancer therapeutics by emphasizing the nature of earthworm extracts and the types of cancer they are used in through a systematic review. A systematic search of CINAHL, PubMed, Medline, Scopus, and Web of Science databases were conducted to identify articles published between January 2018 and December 2022. We utilized various arrangements of the keywords ‘earthworm’, ‘earthworm extract’, and ‘anticancer effect’. We identified original studies written in English depicting in vitro and in vivo cytotoxic impact of worm extracts on cancer cells were included in the review. We found 15 studies matching our search criteria. Among the studies, Eisenia Foetida was determined as the most studied type of earthworm, while Colon cancer was the most frequently studied cancer type. Coleomic Fluid was found to be the commonly used extract to test for anticancer activity with positive results. The study contributes to the literature by providing information about earthworm extracts' antiproliferative and antitumor effects.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140504959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23902/trkjnat.1315772
Liudmyla Dzhus, Tatiana Kovalshuk, інна Діденко, M. Parubok, L. Rozborska
The ecological, edaphic and morphological features and population status of the endemic species Moehringia hypanica Grynj & Klokov were studied within the steppe zone of Ukraine. For the first time, soil analysis, which was taken directly from the places of growth of individuals of the studied species, was carried out. The highest soil content was found in niches under stones (25%), and the lowest in microcracks of granite rocks (5%) of the total volume. The levels of soil supply with mobile nutrients and humus were analysed. The analysis of the cationic and anionic composition of the water extract of the soil showed that M. hypanica belongs to the non-saline soils (the amount of salts does not exceed 0.1%). The content of all salts does not exceed the toxicity thresholds. The results of the soil analysis showed that in order to preserve the populations of these endemic plants, it is necessary to maintain a stable chemical composition of the substrate and prevent human interference. For the first time, it was found that during flowering and fruiting, M. hypanica lacks moisture in the air and substrate. The ability of the plants to grow and develop fruit formation under drought and heat conditions indicates their drought tolerance and thermophilicity. During the expeditionary research, we noted that the populations are dominated by young generative and medium generative plants, with a small number of old generative and virgin individuals. The presence of these aged individuals indicates sufficient seed reproduction. The morphological characteristics of seeds, fruits and age states of M. hypanica plants were determined for the first time.
{"title":"MORPHOSTRUCTURE OF MOEHRINGIA HYPANICA GRYNJ & KLOKOV IN THE NATIONAL NATURE PARK «BUZKY GARD»","authors":"Liudmyla Dzhus, Tatiana Kovalshuk, інна Діденко, M. Parubok, L. Rozborska","doi":"10.23902/trkjnat.1315772","DOIUrl":"https://doi.org/10.23902/trkjnat.1315772","url":null,"abstract":"The ecological, edaphic and morphological features and population status of the endemic species Moehringia hypanica Grynj & Klokov were studied within the steppe zone of Ukraine. For the first time, soil analysis, which was taken directly from the places of growth of individuals of the studied species, was carried out. The highest soil content was found in niches under stones (25%), and the lowest in microcracks of granite rocks (5%) of the total volume. The levels of soil supply with mobile nutrients and humus were analysed. The analysis of the cationic and anionic composition of the water extract of the soil showed that M. hypanica belongs to the non-saline soils (the amount of salts does not exceed 0.1%). The content of all salts does not exceed the toxicity thresholds. The results of the soil analysis showed that in order to preserve the populations of these endemic plants, it is necessary to maintain a stable chemical composition of the substrate and prevent human interference. For the first time, it was found that during flowering and fruiting, M. hypanica lacks moisture in the air and substrate. The ability of the plants to grow and develop fruit formation under drought and heat conditions indicates their drought tolerance and thermophilicity. During the expeditionary research, we noted that the populations are dominated by young generative and medium generative plants, with a small number of old generative and virgin individuals. The presence of these aged individuals indicates sufficient seed reproduction. The morphological characteristics of seeds, fruits and age states of M. hypanica plants were determined for the first time.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140514897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-02DOI: 10.23902/trkjnat.1317293
Muinat Olanike Kazeem, Emmanuel Aduragbemi Adegbemi̇, Abubakar Aisami, I. Onajobi
The use of oily waste as a cost-effective substrate for lipase production is gaining more importance. However, shea-nut cake (SNC) remains under-explored in this regard. Lipases of thermophilic origin such as hot compost bacteria, are of significant biotechnological and industrial importance due to favorable robust properties. This paper describes the isolation of potential lipase producing thermophilic bacteria from hot compost and improvement of its lipase through optimization of nutritional parameters. The bacteria were isolated on tributyrin agar plate and applied for the production of lipase on Olive oil, Shea-nut cake (SNC) and the mixture of olive oil and SNC. Using Plackett-Burman Design (PBD) for screening and Central Composite Design (CCD) of Response Surface Methodology (RSM) for optimization studies, factors influencing lipase production on SNC substrate were identified. One (EAC 9) out of the 4 most potent isolates was identified as Bacillus velezensis EAC9 using 16S rRNA, and produced the highest lipase activity on mixture of olive oil and SNC (103.66 U/ml) than olive oil (65.22) and SNC (41.72 U/ml) alone. Optimization model validity was verified and revealed an optimum medium containing olive oil and Tween 80 at 1.0 % v/v, sucrose of 1.0 % w/v and (NH4)2SO4 of 0.1 % w/v led to maximum lipase production at 200 U/ml which was 4.79-fold increase from the unoptimized. The results indicate that SNC is a better prospective substrate for improving lipase production by thermophilic Bacillus valezensis EAC9, and that statistical methods may be used to deduce optimal parameters for effective lipase production.
{"title":"Utilization of shea-nut cake for lipase production by thermophilic Bacillus valezensis EAC 9 isolated from hot compost and optimization of nutritional parameters","authors":"Muinat Olanike Kazeem, Emmanuel Aduragbemi Adegbemi̇, Abubakar Aisami, I. Onajobi","doi":"10.23902/trkjnat.1317293","DOIUrl":"https://doi.org/10.23902/trkjnat.1317293","url":null,"abstract":"The use of oily waste as a cost-effective substrate for lipase production is gaining more importance. However, shea-nut cake (SNC) remains under-explored in this regard. Lipases of thermophilic origin such as hot compost bacteria, are of significant biotechnological and industrial importance due to favorable robust properties. This paper describes the isolation of potential lipase producing thermophilic bacteria from hot compost and improvement of its lipase through optimization of nutritional parameters. The bacteria were isolated on tributyrin agar plate and applied for the production of lipase on Olive oil, Shea-nut cake (SNC) and the mixture of olive oil and SNC. Using Plackett-Burman Design (PBD) for screening and Central Composite Design (CCD) of Response Surface Methodology (RSM) for optimization studies, factors influencing lipase production on SNC substrate were identified. One (EAC 9) out of the 4 most potent isolates was identified as Bacillus velezensis EAC9 using 16S rRNA, and produced the highest lipase activity on mixture of olive oil and SNC (103.66 U/ml) than olive oil (65.22) and SNC (41.72 U/ml) alone. Optimization model validity was verified and revealed an optimum medium containing olive oil and Tween 80 at 1.0 % v/v, sucrose of 1.0 % w/v and (NH4)2SO4 of 0.1 % w/v led to maximum lipase production at 200 U/ml which was 4.79-fold increase from the unoptimized. The results indicate that SNC is a better prospective substrate for improving lipase production by thermophilic Bacillus valezensis EAC9, and that statistical methods may be used to deduce optimal parameters for effective lipase production.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140515341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-27DOI: 10.23902/trkjnat.1339397
Merve Yılmazer, Semian Karaer Uzuner
Glycogen synthase kinase 3β is a serine/threonine kinase that functions in numerous cellular processes such as cell proliferation, DNA repair, cell cycle, signaling, and metabolic pathways. GSK3β plays a role in several diseases, including inflammation, neurodegenerative disease, diabetes, and cancer. Yeasts are suitable models for the investigation of various cellular processes because they include homologous genes to human genes. In this study, we transferred the human GSK3β gene to Schizosaccharomyces pombe cells (gsk3Δ), including a deletion for this gene. Cells with gsk3 gene deletion and transformant cells with the human GSK3β gene that was reversed by genetic complementation were comparatively examined at the level of gene expression for changes in cellular respiration under varying glucose concentration conditions. For this purpose, the expression of fbp1, pka1 and gsk3 genes were analyzed in cells grown under conditions containing different glucose concentrations. We concluded that the GSK3β gene was expressed more in glucose starvation than in other conditions. We also observed a decrease in the level of gene expression in mitochondrial respiration when the human GSK3β gene was transferred in cells that preferred mitochondrial respiration in the absence of the gsk3 gene, regardless of ambient conditions.
{"title":"Effects of Glucose on the Cellular Respiration in Fission Yeast Expressing Human GSK3β Gene","authors":"Merve Yılmazer, Semian Karaer Uzuner","doi":"10.23902/trkjnat.1339397","DOIUrl":"https://doi.org/10.23902/trkjnat.1339397","url":null,"abstract":"Glycogen synthase kinase 3β is a serine/threonine kinase that functions in numerous cellular processes such as cell proliferation, DNA repair, cell cycle, signaling, and metabolic pathways. GSK3β plays a role in several diseases, including inflammation, neurodegenerative disease, diabetes, and cancer. Yeasts are suitable models for the investigation of various cellular processes because they include homologous genes to human genes. In this study, we transferred the human GSK3β gene to Schizosaccharomyces pombe cells (gsk3Δ), including a deletion for this gene. Cells with gsk3 gene deletion and transformant cells with the human GSK3β gene that was reversed by genetic complementation were comparatively examined at the level of gene expression for changes in cellular respiration under varying glucose concentration conditions. For this purpose, the expression of fbp1, pka1 and gsk3 genes were analyzed in cells grown under conditions containing different glucose concentrations. We concluded that the GSK3β gene was expressed more in glucose starvation than in other conditions. We also observed a decrease in the level of gene expression in mitochondrial respiration when the human GSK3β gene was transferred in cells that preferred mitochondrial respiration in the absence of the gsk3 gene, regardless of ambient conditions.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1288588
Esin POYRAZOĞLU, Mediha ONUR
In this study, the isolation and identification of bacteria that form biofilms on various food products sold on open-air market stalls in Aydın Province were aimed. Bacteria were isolated from food surfaces in aseptic conditions and isolated after incubation in the Tryptic Soy Agar medium at 37°C for 24-48 hours. DNA isolations of the isolated bacteria were performed and the PCR products obtained were used for sequencing. The Congo Red Agar method was used to qualitatively analyze biofilm formation. According to this method, bacteria forming black colonies were evaluated as biofilm-positive and were subjected to quantitative analysis using the Microplate method. A total of 67 bacteria were isolated from the sampled food items, of which 7 were strong and 2 were moderate biofilm producers, showing that more importance should be given to food hygiene.
{"title":"ISOLATION AND IDENTIFICATION OF BIOFILM-FORMING BACTERIA IN THE FOODS SUPPLIED FOR CONSUMPTION AT THE SALES STALLS IN THE PROVINCE OF AYDIN","authors":"Esin POYRAZOĞLU, Mediha ONUR","doi":"10.23902/trkjnat.1288588","DOIUrl":"https://doi.org/10.23902/trkjnat.1288588","url":null,"abstract":"In this study, the isolation and identification of bacteria that form biofilms on various food products sold on open-air market stalls in Aydın Province were aimed. Bacteria were isolated from food surfaces in aseptic conditions and isolated after incubation in the Tryptic Soy Agar medium at 37°C for 24-48 hours. DNA isolations of the isolated bacteria were performed and the PCR products obtained were used for sequencing. The Congo Red Agar method was used to qualitatively analyze biofilm formation. According to this method, bacteria forming black colonies were evaluated as biofilm-positive and were subjected to quantitative analysis using the Microplate method. A total of 67 bacteria were isolated from the sampled food items, of which 7 were strong and 2 were moderate biofilm producers, showing that more importance should be given to food hygiene.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1331537
Ilgaz AKATA, İsmail ŞEN, Ergin SAHİN, Bekir ÇÖL, Emre KESKİN
In the present study, fungal samples collected from Enez and Süloğlu districts of Edirne Province, in Türkiye with the help of truffle-detecting dogs were analyzed. The macroscopic features and environmental details of the collection sites were documented in their natural habitats. The samples were investigated with light- and scanning electron microscopy (SEM) and were also used in ITS rDNA-based molecular phylogenetic analysis, which revealed that they belong to Delastria rosea Tul. & C. Tul. The identification was further supported by morphological data. This is the first record of D. rosea in Türkiye at the genus and species level. A brief description of the newly reported species is provided. Macro- and microphotographs of the spores taken by both ligth and electron microscop (SEM). With this study, the number of genera and species of Turkish truffles and truffle-like fungi has increased to 36 and 105, respectively.
{"title":"Delastria TUL. & C. TUL., A NEW TRUFFLE GENUS RECORD FOR TURKISH MYCOBIOTA","authors":"Ilgaz AKATA, İsmail ŞEN, Ergin SAHİN, Bekir ÇÖL, Emre KESKİN","doi":"10.23902/trkjnat.1331537","DOIUrl":"https://doi.org/10.23902/trkjnat.1331537","url":null,"abstract":"In the present study, fungal samples collected from Enez and Süloğlu districts of Edirne Province, in Türkiye with the help of truffle-detecting dogs were analyzed. The macroscopic features and environmental details of the collection sites were documented in their natural habitats. The samples were investigated with light- and scanning electron microscopy (SEM) and were also used in ITS rDNA-based molecular phylogenetic analysis, which revealed that they belong to Delastria rosea Tul. & C. Tul. The identification was further supported by morphological data. This is the first record of D. rosea in Türkiye at the genus and species level. A brief description of the newly reported species is provided. Macro- and microphotographs of the spores taken by both ligth and electron microscop (SEM). With this study, the number of genera and species of Turkish truffles and truffle-like fungi has increased to 36 and 105, respectively.","PeriodicalId":23163,"journal":{"name":"Trakya University Journal of Natural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.23902/trkjnat.1324202
Metin Burak TATLISES, Semra HASANCEBİ
Substitution of plant cultivars of high commercial value with a cheaper, lower quality one is a common fraud committed against consumers and producers. Since it is one of the most widely grown legumes, lentil (Lens culinaris Medik.) is suitable for such frauds. This study aimed to identify lentil cultivars which are registered and authorized in the market in Türkiye by using current molecular methods. For this purpose, 26 lentil cultivars were analyzed for 15 SSR markers and two DNA barcode regions (trnH-psbA and matK). A high allele diversity was observed by 12 scorable SSR markers, and the average number of alleles was determined to be 16. One of the important findings was the presence of “cultivar-specific alleles” that can be used to identify each cultivar in the lentil market in Türkiye. At least one “cultivar-specific allele” was obtained for each cultivar. The lentil cultivars were also analyzed by two DNA barcode regions as trnH-psbA and matK. While it was observed that the rate of the intra-species variation for the trnH-psbA region was low and 26 varieties were divided into 7 groups, higher rate was found for matK and samples were distributed into 14 groups. Nevertheless, it was observed that intra-species discrimination can be made more effective when both loci are used together and 26 species were distributed into 18 different groups. We expect that the results of this study, especially the cultivar-specific SSR alleles and DNA barcoding sequence data may be used routinely to identify production and packaged products that are commercially available in markets.
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