ANTICANCER ACTIVITIES OF CHITOSAN AND CHITOSAN NANOPARTICLES AGAINST SOME CANCEROUS CELL LINES

Abstract

Cancer is considering a major leading cause of mortality. Nanotechnology holds a gold promise to develop cancer drug delivery and treatment. The present study aimed to compare in-vitro anticancer activities of chitosan (CS) and chitosan nanoparticles (CSN) against MCF7, PC3, HCT116 and A549 cell lines. The nanoparticles physic-chemical properties were estimated by Transmission Electron Microscopy (TEM) and Fourier Transform Infrared analysis (FTIR) through size and zeta potential analysis. In the current study, the results revealed that, the average diameter and zeta potential of CSN were 116.5 nm and 6.43 mV, respectively. Subsequently, CSN showed the anticancer activity with the LC50 values of 101.28 I¼g/mL in MCF7, 367.65 I¼g/mL in PC3, 666.67 I¼g/mL in HCT116 and 681.82 I¼g/mL in A549, whereas CS gave the LC50 values of 102.67 I¼g/mL in MCF7, 694.44 I¼g/mL in PC3, 1470.59 I¼g/mL in HCT116 and 769.23 I¼g/mL in A549. This study concluded that CSN were better than those obtained from CS as anticancer agent