The effect of diazinon exposure on hepatic tissue and enzymes in male frog Rana sridibunda

L. Ghasemzadeh, Hamidreza Mohajereani, S. Nasri, A. Rostami
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引用次数: 2

Abstract

The purpose of this study was to examine the acute and sub-acute effects of diazinon onhistopathological parameters of liver tissue and blood level of hepatic enzymes of male commonfrog (Rana ridibunda). Frogs were assigned to 9 groups (n=8, meaning 8 frogs in each group):control and treatment groups with 30, 60, 90 and 120 μg/L diazinon in water for one day or oneweek. Hepatic enzymes containing alanine transaminase (ALT), aspartate aminotransferase(AST), and alkaline phosphatase (ALP) were measured using biochemical kit. All ALP valueswere significantly different between the control group and one-day and one-week diazinonexposed groups. Hepatocyte changes were observed only for diazinon concentrations greaterthan 60 μl after one week. The most important characteristics of the affected cells werehyperplasia and chromatin formation. Results show that sub-lethal concentrations of diazinonhave an increasing effect on the blood level of hepatic enzymes, which probably indicated thedetoxification response of liver on the basis of diazinon sub-lethal effect model.
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二嗪农暴露对雄性蛙肝组织及酶的影响
本研究旨在观察二嗪农对雄性斑蛙(Rana ridibunda)肝组织病理参数及血酶水平的急性和亚急性影响。将青蛙分为9组(n=8,每组8只):对照组和治疗组,分别在水中添加30、60、90和120 μg/L的二嗪农酯1天或1周。采用生化试剂盒检测肝脏中谷丙转氨酶(ALT)、天冬氨酸转氨酶(AST)和碱性磷酸酶(ALP)的含量。所有ALP值在对照组与暴露1天和1周的二嗪嗪组之间均有显著差异。1周后,只有浓度大于60 μl的二嗪嗪才观察到肝细胞的变化。受影响的细胞最重要的特征是增生和染色质形成。结果表明,亚致死浓度的重氮嗪酮对血中肝脏酶水平的影响增加,这可能是基于重氮嗪酮亚致死效应模型的肝脏解毒反应。
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