Pub Date : 2020-06-26DOI: 10.22059/PBS.2020.302151.1343
M. Larypoor, Ensieh Kargarfaragheh, M. Movahedi
{"title":"Evaluation of the enzymatic activity of halotolerant fungi isolated from Tehran's forest parks","authors":"M. Larypoor, Ensieh Kargarfaragheh, M. Movahedi","doi":"10.22059/PBS.2020.302151.1343","DOIUrl":"https://doi.org/10.22059/PBS.2020.302151.1343","url":null,"abstract":"","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"461 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83071140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-11DOI: 10.22059/PBS.2020.301096.1341
Fariba Mansourizadeh, F. Attari, A. Asadi, A. Nematollahzadeh, Farhad Mansourizadeh
The main goal of tissue engineering is to design scaffolds with the suitable microenvironment, prominent mechanical properties, adequate biocompatibility, and biodegradability. In the present study, Poly-L-lactic acid (PLLA)/10% hydroxyapatite (HA) nano-composite (PLLA/HA) scaffold and pure PLLA scaffold were fabricated via electrospinning technique. They were then assessed for their potential as substrates for the cell culture. The scaffolds were characterized by scanning electron microscope, thermogravimetric analysis, differential thermal analysis, and fourier transform infrared spectroscopy. The results showed the dispersion and integration of HA nanoparticles in the composite scaffold. Pore size distribution measurements indicated that the scaffold pore size in terms of Ferret diameter varies between 0.1 and 22.0 µm. In vitro degradation behavior of the scaffold in phosphate buffered saline (PBS) was obtained by measuring water uptake, weight loss, and pH change of the PBS during the degradation period. We observed that the degradation of the scaffold could be markedly accelerated by HA nanoparticle (10%). It was also shown that acidification of the PBS due to the PLLA degradation is suppressed by the HA nanoparticles. Finally, human umbilical-cord-derived mesenchymal stem cells (hUC-MSCs) were used to evaluate the cellular support of the composite scaffolds. The presence of HA nanoparticles in the PLLA fibrous scaffolds resulted in promoting the attachment and the proliferation of the hUC-MSCs. This study suggests that the PLLA/HA scaffold could be the material of choice for functional bone tissue engineering.
{"title":"Physico-chemical and biological properties of electrospun poly-L-lactic acid/hydroxyapatite nano-composite scaffold for bone regeneration","authors":"Fariba Mansourizadeh, F. Attari, A. Asadi, A. Nematollahzadeh, Farhad Mansourizadeh","doi":"10.22059/PBS.2020.301096.1341","DOIUrl":"https://doi.org/10.22059/PBS.2020.301096.1341","url":null,"abstract":"The main goal of tissue engineering is to design scaffolds with the suitable microenvironment, prominent mechanical properties, adequate biocompatibility, and biodegradability. In the present study, Poly-L-lactic acid (PLLA)/10% hydroxyapatite (HA) nano-composite (PLLA/HA) scaffold and pure PLLA scaffold were fabricated via electrospinning technique. They were then assessed for their potential as substrates for the cell culture. The scaffolds were characterized by scanning electron microscope, thermogravimetric analysis, differential thermal analysis, and fourier transform infrared spectroscopy. The results showed the dispersion and integration of HA nanoparticles in the composite scaffold. Pore size distribution measurements indicated that the scaffold pore size in terms of Ferret diameter varies between 0.1 and 22.0 µm. In vitro degradation behavior of the scaffold in phosphate buffered saline (PBS) was obtained by measuring water uptake, weight loss, and pH change of the PBS during the degradation period. We observed that the degradation of the scaffold could be markedly accelerated by HA nanoparticle (10%). It was also shown that acidification of the PBS due to the PLLA degradation is suppressed by the HA nanoparticles. Finally, human umbilical-cord-derived mesenchymal stem cells (hUC-MSCs) were used to evaluate the cellular support of the composite scaffolds. The presence of HA nanoparticles in the PLLA fibrous scaffolds resulted in promoting the attachment and the proliferation of the hUC-MSCs. This study suggests that the PLLA/HA scaffold could be the material of choice for functional bone tissue engineering.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"129 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74511699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-11DOI: 10.22059/PBS.2020.211595.1223
Jamshid Arum, A. Mohammadi, S. H. Sadraie, S. Sadr, G. Kaka, A. Emamgholi
{"title":"Transected sciatic nerve repair using poly L-lactic-co-glycolic acid nanofiber scaffolds with nerve growth factor in rat","authors":"Jamshid Arum, A. Mohammadi, S. H. Sadraie, S. Sadr, G. Kaka, A. Emamgholi","doi":"10.22059/PBS.2020.211595.1223","DOIUrl":"https://doi.org/10.22059/PBS.2020.211595.1223","url":null,"abstract":"","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73781625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-04DOI: 10.22059/PBS.2019.259788.1305
J. Adesina, K. Ileke, Y. Rajashaker
{"title":"Agricultural wastes: Prospective Entomocides in Management of Cowpea Bruchids Callosobruchus maculatus (Fab.) [Coleoptera: Chrysomelidae]","authors":"J. Adesina, K. Ileke, Y. Rajashaker","doi":"10.22059/PBS.2019.259788.1305","DOIUrl":"https://doi.org/10.22059/PBS.2019.259788.1305","url":null,"abstract":"","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85812107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-04DOI: 10.22059/PBS.2020.258364.1303
E. Salehghamari, F. Taheri, M. Hosseini, M. Sardabi, Alireza Etesami, Gholamheydar Hasani
{"title":"Interspecies interactions of halophilic and halotlerant actinomycetes","authors":"E. Salehghamari, F. Taheri, M. Hosseini, M. Sardabi, Alireza Etesami, Gholamheydar Hasani","doi":"10.22059/PBS.2020.258364.1303","DOIUrl":"https://doi.org/10.22059/PBS.2020.258364.1303","url":null,"abstract":"","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86732096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-18DOI: 10.22059/PBS.2020.275930.1330
R. Bahramabadi, P. Yazdian, H. Hakimi
Recently microbial resistance to antibiotics is worryingly increasing due to the overuse and misuse of antibiotics. Therefore, it seems to be crucial to identify novel antimicrobial agents. Ozone, an unstable and energetic form of oxygen, can be considered as an effective biocide. The goal of the present study was to evaluate the antimicrobial effects of ozone resulting from corona plasma on some pathogenic bacteria. In the present study, three opportunistic pathogenic ubiquitous bacteria; Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus were obtained from the Collection Center of Industrial Fungi and Bacteria of Iran. Different concentrations of the bacteria were cultured on the Muller Hinton agar medium, and left at room temperature for 5 minutes. Then, they received ozone produced by corona plasma-generating device for 0, 5, 10, and 15 minutes. Finally, they were incubated at 37°C for 24 hours to compare the colony counts. Our study revealed that ozone was able to inhibit the growth of all examined bacteria as the number of colony forming units (CFUs) were significantly decreased. In conclusion, according to the findings of the present study, ozone can be considered a beneficial and comprehensive antimicrobial agent against pathogenic bacteria.
{"title":"Antibacterial effects of ozone generated in corona air plasma","authors":"R. Bahramabadi, P. Yazdian, H. Hakimi","doi":"10.22059/PBS.2020.275930.1330","DOIUrl":"https://doi.org/10.22059/PBS.2020.275930.1330","url":null,"abstract":"Recently microbial resistance to antibiotics is worryingly increasing due to the overuse and misuse of antibiotics. Therefore, it seems to be crucial to identify novel antimicrobial agents. Ozone, an unstable and energetic form of oxygen, can be considered as an effective biocide. The goal of the present study was to evaluate the antimicrobial effects of ozone resulting from corona plasma on some pathogenic bacteria. In the present study, three opportunistic pathogenic ubiquitous bacteria; Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus were obtained from the Collection Center of Industrial Fungi and Bacteria of Iran. Different concentrations of the bacteria were cultured on the Muller Hinton agar medium, and left at room temperature for 5 minutes. Then, they received ozone produced by corona plasma-generating device for 0, 5, 10, and 15 minutes. Finally, they were incubated at 37°C for 24 hours to compare the colony counts. Our study revealed that ozone was able to inhibit the growth of all examined bacteria as the number of colony forming units (CFUs) were significantly decreased. In conclusion, according to the findings of the present study, ozone can be considered a beneficial and comprehensive antimicrobial agent against pathogenic bacteria.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83542466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-18DOI: 10.22059/PBS.2020.226587.1252
R. Haidari
{"title":"A new sampling method for forest characteristics estimation: A Case study Kermanshah Province, Iran","authors":"R. Haidari","doi":"10.22059/PBS.2020.226587.1252","DOIUrl":"https://doi.org/10.22059/PBS.2020.226587.1252","url":null,"abstract":"","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"38 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91472405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.22059/PBS.2018.247627.1291
M. Moezipour, Marjan Seiedy, J. Nozari, R. Malheiro
Olive fly is the most dangerous pest in olive groves worldwide. Therefore the study of the most susceptible and resistant cultivars to olive fly can bring new information to diminish the olive flies harmful impacts. The main goal of the present study is to verify the olfactory response of olive fly to olive volatiles from five native Iranian cultivars (Fishomi, Mari, Rowghani, Shengeh, and Zard) and four exotic cultivars (Arbequina, Coratina, Koroneiki, and Manzanilla). Olfactometer bioassays were carried out in order to verify the attraction level of the volatiles of different cultivars to olive flies. A second experiment was performed with native cultivars in order to verify the preference between healthy olives and olives already infested by olive fly. The obtained results demonstrated that among native cultivars, Fishomi and Zard were those attracting higher number of olive flies, while cv. Rowghani showed to be the less preferred one. The exotic olive cultivars, Arbequina and Manzanilla attracted higher number of olive flies, while the volatiles of cv. Koroneiki showed a low attraction effect. According to the results of this study we suggest setting new strategies in cultivation of olives by spreading those cultivars less attractive to olive fly
{"title":"Reduction of olive fly, Bactrocera oleae (Rossi, 1790), damage by selecting native and exotic olive cultivars","authors":"M. Moezipour, Marjan Seiedy, J. Nozari, R. Malheiro","doi":"10.22059/PBS.2018.247627.1291","DOIUrl":"https://doi.org/10.22059/PBS.2018.247627.1291","url":null,"abstract":"Olive fly is the most dangerous pest in olive groves worldwide. Therefore the study of the most susceptible and resistant cultivars to olive fly can bring new information to diminish the olive flies harmful impacts. The main goal of the present study is to verify the olfactory response of olive fly to olive volatiles from five native Iranian cultivars (Fishomi, Mari, Rowghani, Shengeh, and Zard) and four exotic cultivars (Arbequina, Coratina, Koroneiki, and Manzanilla). Olfactometer bioassays were carried out in order to verify the attraction level of the volatiles of different cultivars to olive flies. A second experiment was performed with native cultivars in order to verify the preference between healthy olives and olives already infested by olive fly. The obtained results demonstrated that among native cultivars, Fishomi and Zard were those attracting higher number of olive flies, while cv. Rowghani showed to be the less preferred one. The exotic olive cultivars, Arbequina and Manzanilla attracted higher number of olive flies, while the volatiles of cv. Koroneiki showed a low attraction effect. According to the results of this study we suggest setting new strategies in cultivation of olives by spreading those cultivars less attractive to olive fly","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"146 1","pages":"87-95"},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77650513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-19DOI: 10.22059/PBS.2016.590020
Javad Karimi, S. Mohsenzadeh
The extensive use of heavy metals and nanoparticles (NPs) has led to their release into the environment that might have negative impacts on both organisms and the environment. In this study, the molecular responses of wheat seedlings to silver nitrate and silver nanoparticles (AgNPs) were assessed by transcript accumulation analysis of genes coding for products potentially involved in heavy metal tolerance. A quantitative Real-time PCR experiment was performed with MAPK (Mitogen-activated protein kinase) and thioredoxin genes using RNA isolated from wheat shoots treated for 0, 2, 6, 12 and 24 h with AgNO3 and AgNPs at 100 mg-1L concentration. Results indicated that stressful conditions led to the antioxidant responses of wheat seedlings that could be reflected as changes in MAPK and thioredoxin gene transcripts. The genes expression patterns were slightly different. The expression of these genes in response to both treatments was high at the beginning of the stress and was decreased with time. Our results showed the effects of AgNO3 treatment were faster than AgNPs. We found that wheat seedlings might develop different strategies to cope with AgNO3 and AgNPs toxicity with change in the expression of MAPK and thioredoxin heavy metal-related genes.
{"title":"Comparison of MAPK and thioredoxin gene expression in wheat seedlings exposed to silver nitrate and silver nanoparticle","authors":"Javad Karimi, S. Mohsenzadeh","doi":"10.22059/PBS.2016.590020","DOIUrl":"https://doi.org/10.22059/PBS.2016.590020","url":null,"abstract":"The extensive use of heavy metals and nanoparticles (NPs) has led to their release into the environment that might have negative impacts on both organisms and the environment. In this study, the molecular responses of wheat seedlings to silver nitrate and silver nanoparticles (AgNPs) were assessed by transcript accumulation analysis of genes coding for products potentially involved in heavy metal tolerance. A quantitative Real-time PCR experiment was performed with MAPK (Mitogen-activated protein kinase) and thioredoxin genes using RNA isolated from wheat shoots treated for 0, 2, 6, 12 and 24 h with AgNO3 and AgNPs at 100 mg-1L concentration. Results indicated that stressful conditions led to the antioxidant responses of wheat seedlings that could be reflected as changes in MAPK and thioredoxin gene transcripts. The genes expression patterns were slightly different. The expression of these genes in response to both treatments was high at the beginning of the stress and was decreased with time. Our results showed the effects of AgNO3 treatment were faster than AgNPs. We found that wheat seedlings might develop different strategies to cope with AgNO3 and AgNPs toxicity with change in the expression of MAPK and thioredoxin heavy metal-related genes.","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"47 1","pages":"189-196"},"PeriodicalIF":0.0,"publicationDate":"2017-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76851344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-12-19DOI: 10.22059/PBS.2016.590022
H. Amiri, A. Ghiasvand
The composition of hydro-distilled essential oils of Phlomis anisodonta Boiss. subsp. occidentalis Jamzad in vegetative, flowering and post flowering stages were investigated using GC and GC-MS, leading to identification of 41, 26 and 23 compounds, respectively. In all three samples, sesquiterpenes were the main components. In vegetative stage the main components of the oil were germacrene-D (14.3%), bicyclogermacrene (12.4%) and α-pinene (6.8%); in flowering stage, germacrene-D (52.6%) and β-caryophyllene (15.9%); and in fruiting stage, germacrene-D (27.9%), bicyclogermacrene (17.6%), caryophyllene oxide (14.7%) and β-caryophyllene (11.3%). The samples were also subjected to screening for their possible antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid assays. In the first experiment, the free radical scavenging activity of polar subfraction of methanol extract in fruiting stage was superior to all other extracts (IC50= 41±0.4 μg/ml). When using linoleic acid system, oxidation of the linoleic acid was effectively inhibited by the non-polar subfraction of methanol extract in different stages. The results of this study show that the composition of essential oils varies considerably both in different parts of the plants and at different stages of development. When using the plants for medicinal purposes, it is important to be aware of the effective developmental phase, i.e. when the most effective components reach their peak volume, as well as the most useful parts. Phenology, Antioxidant activity, Volatile oils, Germacrene-D, Bicyclogermcrene
{"title":"Changes in composition and antioxidant activities of essential oils in Phlomis anisodonta (Lamiaceae) at different stages of maturity","authors":"H. Amiri, A. Ghiasvand","doi":"10.22059/PBS.2016.590022","DOIUrl":"https://doi.org/10.22059/PBS.2016.590022","url":null,"abstract":"The composition of hydro-distilled essential oils of Phlomis anisodonta Boiss. subsp. occidentalis Jamzad in vegetative, flowering and post flowering stages were investigated using GC and GC-MS, leading to identification of 41, 26 and 23 compounds, respectively. In all three samples, sesquiterpenes were the main components. In vegetative stage the main components of the oil were germacrene-D (14.3%), bicyclogermacrene (12.4%) and α-pinene (6.8%); in flowering stage, germacrene-D (52.6%) and β-caryophyllene (15.9%); and in fruiting stage, germacrene-D (27.9%), bicyclogermacrene (17.6%), caryophyllene oxide (14.7%) and β-caryophyllene (11.3%). The samples were also subjected to screening for their possible antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid assays. In the first experiment, the free radical scavenging activity of polar subfraction of methanol extract in fruiting stage was superior to all other extracts (IC50= 41±0.4 μg/ml). When using linoleic acid system, oxidation of the linoleic acid was effectively inhibited by the non-polar subfraction of methanol extract in different stages. The results of this study show that the composition of essential oils varies considerably both in different parts of the plants and at different stages of development. When using the plants for medicinal purposes, it is important to be aware of the effective developmental phase, i.e. when the most effective components reach their peak volume, as well as the most useful parts. Phenology, Antioxidant activity, Volatile oils, Germacrene-D, Bicyclogermcrene","PeriodicalId":20726,"journal":{"name":"Progress in Biological Sciences","volume":"17 1","pages":"205-212"},"PeriodicalIF":0.0,"publicationDate":"2017-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81153772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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