Integrated Quantitative Estimation of Neuro- Endocrine Manifestations of Chronic Stress in Female Rats

Abstract

Previously in line with the concept of a triune neuro-endocrine-immune complex [3, 5, 7, 9] we have been carried out integrated quantitative estimation of neuroendocrine and immune responses to chronic restraint stress at male rats. The method of discriminant analysis has found that distinctive endocrine signs of chronic stress are increasing thickness of Fasciculary Zone whereas decreasing thickness of Glomerulary Zone of Adrenal Cortex as well as plasma (Ca/P) 0,5 ratio as Parathyrine Activity. Other signs of chronic stress such as increasing plasma levels Corticosterone, Testosterone and Triiodo-thyronine, Sympathetic tone, Heart Rate and thickness of Reticular Zone of Adrenals as well as decreasing Vagal Tone and plasma (Na/K) 0.5 ratio as Mineralocorticoide Activity currently not in the discriminant model. Canonical Neuroendocrine Roots for Intact and Stressed Males Rats averages +0.99 ± 0.40 and -0.25 ± 0.15 respectively (Squared Mahalanobis Distance D 2M = 1.61; F = 3.76; p = 0.017). Among the parameters of Immunity characteristic of chronic stress appeared to increase Thymus Massa Index, level in Thymocytogram of Macrophages and Reticulocytes, in Splenocytogram of Macrophages and Eosinophils, Monocytes in Leukocytogram of Blood have increased as well as Entropy of Leukocytogram and Splenocytogram whereas both Intensivity and Activity of Phagocytose by Neutrophils, levels of Endotheliocytes in Thymocytogram, Neutrophils in Splenocytogram, Background . Previously in line with the concept of a triune neuro-endocrine-immune complex we have been carried out integrated quantitative estimation of neuroendocrine and immune responses to chronic restraint stress at male rats. At the next stage, research has been conducted in this line on female rats. In this article we give the results of quantitative evaluation of neuroendocrine responses to chronic restraint stress. Material and methods. The experiment has been conducted on 60 white female rats Wistar line weighing 230-300 g. 10 of these animals have not subjected to any influences and 50 of them subjected to moderated stress by daily 30-minute immobilization during 7 days. The day after the completion of stressing in rats of both groups the parameters of HRV, blood levels of hormones as well as blood and urine levels Ca, P, Na and K have been registered. The thickness of glomerular, fascicular and reticular zones in sections of the adrenal glands thas been measured under a microscope. Results. It has been found that the mineralocorticoid activity, which is estimated by the Na/K ratio of plasma, increases to the maximum. Further, in the descending order, follow: testos-teroneemia, thickness of the fascicular zone of the adrenal cortex (ZAC), heart rate, sympathetic tone, thickness of the reticular ZAC and plasma level of triiodothyronine. Hormonal constellation consisting of calcitonin activity (calculated by the formula: (Cau•Pu/Pp•Cap) 0.25 ), adrenals mass, thickness of the glomerular ZAC, corticosteroneemia and vagal tone is not significantly different from that of the control. In contrast, thyroxinemia and paratyrin activity (calculated by the formula: (Caр•Pu/Pp•Cau) 0.25 ) have been significantly decreased. The integral quantitative measure of the neuroendocrine response to the factors of chronic stress as the mean value of the significant modules of Z-units is 0.61 ± 0.10, that is, it is not significantly different from revealed earlier by males: 0.47 ± 0.04. However, there are sexual differences between the post stressed neuro-endocrine profiles not only in the severity of reactions, but also in their sign.