Pub Date : 2020-01-15DOI: 10.25040/ecpb2019.04.005
O. Mel’nyk, Z. Struk
Introduction. Earlier in the experiments in rats [1,2] and in clinical observations [3–5] showed that the immune responses to chronic stress are ambiguous and individual. Adaptogens, in particular balneofactors, are antipodes of stressors [6]. The immune responses to balneotherapy are also ambiguous [7–19] which is a separate manifestation of the multivariate effects of balneological agents on the body [20,21]. Therefore, the purpose of this study is to analyze variants of immune responses to balneofactors of Truskavets’ spa as natural adaptogens and to determine the possibility of their prediction. Material and methods. The object of observation were 34 men and 10 women aged 24–70 years old, who came to the Truskavets’ spa for the treatment of chronic pyelonephritis combined with cholecystitis in remission. The survey was conducted twice, before and after balneotherapy (drinking bioactive water Naftussya three times a day, ozokerite applications, mineral baths every other day for 7–10 days). Immune status evaluated on a set of I and II levels recommended by the WHO as described in the manuals [22,23]. For phenotyping subpopulations of lymphocytes used the methods of rosette formation with sheep erythrocytes on which adsorbed monoclonal antibodies against receptors CD3, CD4, CD8, CD22 and CD56 from company «Granum» (Kharkiv) with visualization under light microscope with immersion system. Subpopulation of T cells with receptors high affinity determined by test of “active” rosette formation. The state of humoral immunity judged by the concentration in serum of Circulating Immune Complexes (by polyethylene glycol precipitation method) and Immunoglobulins classes M, G, A as well as in saliva of IgG, IgA, secretory IgA and Lysozyme (ELISA, analyser “Immunochem”, USA). Parameters of phagocytic function of neutrophils estimated as described by SD. Douglas and PG Quie [24] with moderately modification by MM Kovbasnyuk [25]. The objects of phagocytosis served daily cultures of Staphylococcus aureus (ATCC N 25423 F49) as typical specimen for Gram-positive Bacteria and Escherichia coli (O55 K59) as typical representative of Gram-negative Bacteria. Both cultures obtained from Laboratory of Hydro-Geological Regime-Operational Station JSC “Truskavets’kurort”. Take into account the following parameters of Phagocytosis: activity (percentage of neutrophils, in which found microbes – Hamburger’s Phagocytic Index PhI), intensity (number of microbes absorbed one phagocytes – Microbial Count MC or Right’s Index) and completeness
介绍。早期的大鼠实验[1,2]和临床观察[3-5]表明，对慢性应激的免疫反应是模糊的和个体的。适应原，特别是平衡因子，是应激源的对跖物。对浴疗的免疫反应也是不明确的[7-19]，这是浴疗药物对机体多因素影响的一个单独表现[20,21]。因此，本研究的目的是分析Truskavets ' spa balnefactors作为天然适应原的免疫应答变异，并确定其预测的可能性。材料和方法。观察对象为24-70岁来Truskavets水疗中心治疗慢性肾盂肾炎合并胆囊炎缓解期的34男10女。该调查在浴疗前后进行了两次(每天三次饮用Naftussya生物活性水，ozokerite应用，每隔一天泡一次矿泉浴，持续7-10天)。根据手册[22,23]中描述的世界卫生组织推荐的一组I和II水平评估免疫状况。对于淋巴细胞亚群的表型分型，采用羊红细胞花环形成的方法，在其上吸附来自«Granum»(Kharkiv)公司的抗CD3、CD4、CD8、CD22和CD56受体的单克隆抗体，在光镜下用浸泡系统显示。具有高亲和力受体的T细胞亚群通过“活性”莲座形成试验确定。通过血清循环免疫复合物(聚乙二醇沉淀法)和免疫球蛋白M、G、A类以及唾液中IgG、IgA、分泌IgA和溶菌酶(ELISA，美国“Immunochem”分析仪)的浓度判断体液免疫状态。中性粒细胞吞噬功能参数用SD描述。Douglas和PG Quie [24]， MM Kovbasnyuk[25]进行了适度修改。吞噬对象为每日培养的金黄色葡萄球菌(ATCC N 25423 F49)为革兰氏阳性菌的典型标本，大肠杆菌(O55 K59)为革兰氏阴性菌的典型代表。两种培养物均来自水文地质制度实验室-“Truskavets’kurort”JSC运营站。考虑吞噬作用的以下参数:活性(中性粒细胞的百分比，其中发现微生物-汉堡包的吞噬指数PhI)，强度(一个吞噬细胞吸收的微生物数量-微生物计数MC或赖特指数)和完整性
{"title":"Predictors of individual immune responses to adaptogens","authors":"O. Mel’nyk, Z. Struk","doi":"10.25040/ecpb2019.04.005","DOIUrl":"https://doi.org/10.25040/ecpb2019.04.005","url":null,"abstract":"Introduction. Earlier in the experiments in rats [1,2] and in clinical observations [3–5] showed that the immune responses to chronic stress are ambiguous and individual. Adaptogens, in particular balneofactors, are antipodes of stressors [6]. The immune responses to balneotherapy are also ambiguous [7–19] which is a separate manifestation of the multivariate effects of balneological agents on the body [20,21]. Therefore, the purpose of this study is to analyze variants of immune responses to balneofactors of Truskavets’ spa as natural adaptogens and to determine the possibility of their prediction. Material and methods. The object of observation were 34 men and 10 women aged 24–70 years old, who came to the Truskavets’ spa for the treatment of chronic pyelonephritis combined with cholecystitis in remission. The survey was conducted twice, before and after balneotherapy (drinking bioactive water Naftussya three times a day, ozokerite applications, mineral baths every other day for 7–10 days). Immune status evaluated on a set of I and II levels recommended by the WHO as described in the manuals [22,23]. For phenotyping subpopulations of lymphocytes used the methods of rosette formation with sheep erythrocytes on which adsorbed monoclonal antibodies against receptors CD3, CD4, CD8, CD22 and CD56 from company «Granum» (Kharkiv) with visualization under light microscope with immersion system. Subpopulation of T cells with receptors high affinity determined by test of “active” rosette formation. The state of humoral immunity judged by the concentration in serum of Circulating Immune Complexes (by polyethylene glycol precipitation method) and Immunoglobulins classes M, G, A as well as in saliva of IgG, IgA, secretory IgA and Lysozyme (ELISA, analyser “Immunochem”, USA). Parameters of phagocytic function of neutrophils estimated as described by SD. Douglas and PG Quie [24] with moderately modification by MM Kovbasnyuk [25]. The objects of phagocytosis served daily cultures of Staphylococcus aureus (ATCC N 25423 F49) as typical specimen for Gram-positive Bacteria and Escherichia coli (O55 K59) as typical representative of Gram-negative Bacteria. Both cultures obtained from Laboratory of Hydro-Geological Regime-Operational Station JSC “Truskavets’kurort”. Take into account the following parameters of Phagocytosis: activity (percentage of neutrophils, in which found microbes – Hamburger’s Phagocytic Index PhI), intensity (number of microbes absorbed one phagocytes – Microbial Count MC or Right’s Index) and completeness","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74465473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.005
Paramita Bhattacharyya, D. Mathangi, P. Johnson
PARAMITA BHATTACHARYYA1, MATHANGI D.C.2, PRICILLA JOHNSON3 1 Associate Professor of Physiology, Pondicherry Institute of Medical Sciences, Pondicherry, India 2 Professor of Physiology, Chettinad Hospital and Research Centre, Kelambakkam, Chennai, India 3 Professor of Physiology, Sri Ramachandra University,Chennai, India The name of the institution where the work has been done: Department of Physiology, Sri Ramachandra University, Chennai, India
{"title":"Assessment of Maternal Oxidative Stress and Antioxidant Defence during Caesarean section","authors":"Paramita Bhattacharyya, D. Mathangi, P. Johnson","doi":"10.25040/ECPB2018.01.005","DOIUrl":"https://doi.org/10.25040/ECPB2018.01.005","url":null,"abstract":"PARAMITA BHATTACHARYYA1, MATHANGI D.C.2, PRICILLA JOHNSON3 1 Associate Professor of Physiology, Pondicherry Institute of Medical Sciences, Pondicherry, India 2 Professor of Physiology, Chettinad Hospital and Research Centre, Kelambakkam, Chennai, India 3 Professor of Physiology, Sri Ramachandra University,Chennai, India The name of the institution where the work has been done: Department of Physiology, Sri Ramachandra University, Chennai, India","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82845407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.051
N. I. Kupynyak, O. Ikkert, V. V. Man'ko
{"title":"Energy processes in rat liver under ryanodine","authors":"N. I. Kupynyak, O. Ikkert, V. V. Man'ko","doi":"10.25040/ECPB2018.01.051","DOIUrl":"https://doi.org/10.25040/ECPB2018.01.051","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88906369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.074
V. Pyrohova, K. Kozak
{"title":"The prevalence of vitamin D insufficiency/deficiency among women of the perimenopausal period in the western region of Ukraine","authors":"V. Pyrohova, K. Kozak","doi":"10.25040/ECPB2018.01.074","DOIUrl":"https://doi.org/10.25040/ECPB2018.01.074","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80839035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.033
R. Fafula, О. Meskalo, E. Lychkovskyy, Z. Vorobets
Introduction. It is known that mammalian and human spermatozoa are able to generate reactive oxygen species (ROS) which are important regulators of sperm functions (hyperactivation, capacitation and acrosome reaction). However, an excessive ROS generation appears to be related to male infertility. An uncontrolled production of ROS or impaired antioxidant defense enzyme and non-enzyme mechanisms leads to oxidative stress which is harmful to sperm cells. It has been demonstrated recently that oxidative stress is implicated in male infertility. ROS-mediated damage to sperm is a considerable pathology contribution in 30% to 80% of unselected infertile patients [1, 2]. One of the most common ROS having potential implications in the reproductive biology is hydrogen peroxide (H2O2). It was shown that human spermatozoa incubated and exposed to artificial H2O2 has detrimental effects on sperm motility and give rise to a significant increase in the overall ROS [4]. Oxidative stress may have negative impact on the activity of membranebound enzymes such as Са2+, Мg2+-АТPase which is involved in maintaining calcium homeostasis in spermatozoa. Ion-transporting ATPases are very sensitive to oxidation. The inhibition of Са2+, Мg2+-АТPase may occur through a different mechanism [9, 12]. The aim of the present work was to evaluate the H2O2 effect on the main kinetic parameters of ATP hydrolysis by thapsigargin-sensitive Са2+, Мg2+-АТPase of spermatozoa of fertile (normozoospermia) and infertilite men (asthenozoospermia). Material and Methods. Patients. 10 infertile men with asthenozoospermia were involved in this study. The main exclusion criteria: men who were currently on any medication or antioxidant supplementation were not included. In addition, men with infertility over 10 years, azoospermia, genital infection, chronic illness and serious systemic diseases, smokers and alcoholic men were excluded from the study because of their well-known high seminal ROS levels and decreased antioxidant activity which may affect ATPase activities. Infertile men were age-matched to fertile control cases. The control group consisted of 8 healthy men with somatic fertility, normozoospermia and confirmed parenthood (married for 3–10 years and have 1–3 healthy children). Semen samples were obtained by masturbation and collected into sterile containers, following 3–5 days of abstinence from sexual activity. After liquefaction at 37 °C with 5 % CO2 in air, semen samples were examined for volume, sperm concentration, pH, morphology and motility according to World Health Organization guidelines (WHO, 2010) [11]. Before turning to study, all men were aware of patient information leaflets and gave informed consent to participate in the research. Terms of sample selection meet the requirements of
{"title":"Hydrogen Peroxide Effect on the Thapsigargin-sensitive Cа2+, Mg2+- ATPase Activity of Sperm Cells","authors":"R. Fafula, О. Meskalo, E. Lychkovskyy, Z. Vorobets","doi":"10.25040/ECPB2018.01.033","DOIUrl":"https://doi.org/10.25040/ECPB2018.01.033","url":null,"abstract":"Introduction. It is known that mammalian and human spermatozoa are able to generate reactive oxygen species (ROS) which are important regulators of sperm functions (hyperactivation, capacitation and acrosome reaction). However, an excessive ROS generation appears to be related to male infertility. An uncontrolled production of ROS or impaired antioxidant defense enzyme and non-enzyme mechanisms leads to oxidative stress which is harmful to sperm cells. It has been demonstrated recently that oxidative stress is implicated in male infertility. ROS-mediated damage to sperm is a considerable pathology contribution in 30% to 80% of unselected infertile patients [1, 2]. One of the most common ROS having potential implications in the reproductive biology is hydrogen peroxide (H2O2). It was shown that human spermatozoa incubated and exposed to artificial H2O2 has detrimental effects on sperm motility and give rise to a significant increase in the overall ROS [4]. Oxidative stress may have negative impact on the activity of membranebound enzymes such as Са2+, Мg2+-АТPase which is involved in maintaining calcium homeostasis in spermatozoa. Ion-transporting ATPases are very sensitive to oxidation. The inhibition of Са2+, Мg2+-АТPase may occur through a different mechanism [9, 12]. The aim of the present work was to evaluate the H2O2 effect on the main kinetic parameters of ATP hydrolysis by thapsigargin-sensitive Са2+, Мg2+-АТPase of spermatozoa of fertile (normozoospermia) and infertilite men (asthenozoospermia). Material and Methods. Patients. 10 infertile men with asthenozoospermia were involved in this study. The main exclusion criteria: men who were currently on any medication or antioxidant supplementation were not included. In addition, men with infertility over 10 years, azoospermia, genital infection, chronic illness and serious systemic diseases, smokers and alcoholic men were excluded from the study because of their well-known high seminal ROS levels and decreased antioxidant activity which may affect ATPase activities. Infertile men were age-matched to fertile control cases. The control group consisted of 8 healthy men with somatic fertility, normozoospermia and confirmed parenthood (married for 3–10 years and have 1–3 healthy children). Semen samples were obtained by masturbation and collected into sterile containers, following 3–5 days of abstinence from sexual activity. After liquefaction at 37 °C with 5 % CO2 in air, semen samples were examined for volume, sperm concentration, pH, morphology and motility according to World Health Organization guidelines (WHO, 2010) [11]. Before turning to study, all men were aware of patient information leaflets and gave informed consent to participate in the research. Terms of sample selection meet the requirements of","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87928563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.062
S. Cherkasov
{"title":"Interdependence between corticospinal innervation and the development of segmental hyperexcitability, in patients after hemispheric ischemic stroke","authors":"S. Cherkasov","doi":"10.25040/ECPB2018.01.062","DOIUrl":"https://doi.org/10.25040/ECPB2018.01.062","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74412716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.027
K. Tamilselvan, N. Nirmala, R. Latha, U. K. Priyadharshini, S. Niveatha, J. Samuel
K. TAMILSELVAN1, N. NIRMALA2, R. LATHA3, U. KARTHIKA PRIYADHARSHINI4, S. NIVEATHA5, J. GODSON TITUS SAMUEL6 1 Associate Professor, Department of Physiology, SVMCH &RC, Puducherry 2 Professor & HOD, Department of Physiology, SVMCH &RC, Puducherry 3 Professor, Department of Physiology, SVMCH &RC, Puducherry 4 Assistant Professor, Department of Physiology, SVMCH &RC, Puducherry 5 II year post-graduate, Department of Physiology, SVMCH &RC, Puducherry 6 III year M.B.B.S., Sri Venkateshwaraa Medical College Hospital and Research Centre (SVMCH&RC), Ariyur, Puducherry, India
K. TAMILSELVAN1, N. NIRMALA2, R. LATHA3, U. KARTHIKA PRIYADHARSHINI4, S. NIVEATHA5, J. GODSON TITUS SAMUEL6 1瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系副教授，瑞典国立国立大学与研究中心生理学系硕士，瑞典国立国立大学与研究中心生理学系硕士，瑞典国立国立大学生理学系硕士，瑞典国立国立大学生理学系硕士，瑞典国立国立大学生理学系硕士，瑞典国立国立大学生理学系硕士，瑞典国立国立大学生理学系硕士，瑞典国立国立大学生理学系硕士。Sri Venkateshwaraa医学院医院和研究中心(SVMCH&RC)，印度普杜切里阿里尤尔
{"title":"Assessment of Nerve conduction in non-diabetic offspring of type 2 diabetic parents - a pilot study","authors":"K. Tamilselvan, N. Nirmala, R. Latha, U. K. Priyadharshini, S. Niveatha, J. Samuel","doi":"10.25040/ECPB2018.01.027","DOIUrl":"https://doi.org/10.25040/ECPB2018.01.027","url":null,"abstract":"K. TAMILSELVAN1, N. NIRMALA2, R. LATHA3, U. KARTHIKA PRIYADHARSHINI4, S. NIVEATHA5, J. GODSON TITUS SAMUEL6 1 Associate Professor, Department of Physiology, SVMCH &RC, Puducherry 2 Professor & HOD, Department of Physiology, SVMCH &RC, Puducherry 3 Professor, Department of Physiology, SVMCH &RC, Puducherry 4 Assistant Professor, Department of Physiology, SVMCH &RC, Puducherry 5 II year post-graduate, Department of Physiology, SVMCH &RC, Puducherry 6 III year M.B.B.S., Sri Venkateshwaraa Medical College Hospital and Research Centre (SVMCH&RC), Ariyur, Puducherry, India","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91468628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.068
S. Paryzhak, T. Dumych, R. Bilyy
{"title":"Pathways of neutrophil activation by natural hydrophobic nanocrystals","authors":"S. Paryzhak, T. Dumych, R. Bilyy","doi":"10.25040/ECPB2018.01.068","DOIUrl":"https://doi.org/10.25040/ECPB2018.01.068","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90952251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.011
I. Popovych
Introduction. In previous studies of the Truskavets’ Scientific School of Balneology, it has been shown that in response to the use of Bioactive Water Naftussya (BAWN) the activity of the autonomic nervous system changes. However, the nature of the reaction is ambiguous and is conditioned by the constellation of the initial parameters of the body [15,16,24,28]. The immediate reactions of diuresis, gastric and pancreatic secretion, hemodynamics were also interspersed [2, 6, 9, 27, 28, 31]. In the context of the adaptogenic concept of the mechanism of action BAWN [22], it is also shown that the polyversive reactions of the autonomic nervous system are accompanied by changes in some parameters of the electroencephalogram, immunogram of blood and levels of adaptation hormones in it [14-16, 24, 28]. However, the issue of the specificity of immediate effects of BAWN on the nervous, endocrine and immune systems, that function interconnected as a triple complex [15, 21, 22, 28], is still relevant. The answer to this question can be obtained by comparing the reactions of the autonomic nervous system to BAWN with reactions to ordinary (daily) water on the one hand, and other natural adaptogens on the other hand. This is the objective of this study. Material and Methods. The objects of the observation were 15 volunteers-men (aged 26÷60 years, M±SD: 44±12 years) without any clinical diagnose but with a moderate dysfunction of neuroendocrine-immune complex (dysadaptation). At first volunteers filled in a questionnaire with the purpose of estimation of the level of the trait anxiety [29]. Then EEG was recorded for 25 sec by means of the hardware-software complex ‘NeuroCom Standard’ (producer KhAI Medica, Kharkiv, Ukraine) monopolar in 16 loci (Fp1, Fp2, F3, F4, F7, F8, C3, C4, T3, T4, P3, P4, T5, T6, O1, O2) by 10-20 international system, with the reference electrodes A and Ref on tassels of the ears. Among the options the average EEG amplitude (μV) was considered as well as the modal frequency (Hz), frequency deviation (Hz), index (%), coefficient of asymmetry (%), absolute (μV2/Hz) and relative (%) power spectrum density (SPD) of basic rhythms: β (35÷13 Hz), α (13÷8 Hz), θ (8÷4 Hz) and δ (4÷0,5 Hz) in all loci, according to the instructions of the device. In addition, the Laterality Index (LI) for SPD of each rhythm was calculated using the formula [18]: LI, % = Σ [200•(Right – Left)/(Right + Left)]/8. For each locus the Entropy (h) of normalized SPD was also calculated by using classical CE Shannon’s formula [30]: h= [SPDα•log2 SPDα + SPDβ•log2 SPDβ + SPDθ•log2 SPDθ + + SPDδ•log2 SPDδ]/log2 4
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Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.079
A. Ocheretniuk, B. Kondratskyi, O. Palamarchuk, V. Vashchuk
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