Development and detection efficiency of sequence characterized amplified region markers for authentication of medicinal plant Ruta graveolens and its adulterant Euphorbia dracunculoides

Drug Development and Therapeutics Pub Date : 2016-07-01 DOI:10.4103/2394-6555.191172

Irum Gul, Athar Ali, K. J. Mirza, M. Abdin

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Abstract

Background: With the increase in demand of herbal medicines, adulteration in these drugs is also gaining momentum and remains an indispensable problem in domestic and export markets. Correct identification is the first step toward assuring quality, safety, and efficacy of indigenous herbal medicines. Materials and Methods: In this study, sequence characterized amplified region (SCAR) markers were developed to discriminate Ruta graveolens from its adulterant Euphorbia dracunculoides. Random amplified polymorphic DNA (RAPD) was performed and subsequently converted into SCAR markers. Results: After performing RAPD, SCAR primers were designed from the selected unique RAPD amplicons of the genuine drug as well as its adulterant. These primers produced 670 bp and 750 bp SCAR markers with genomic DNA sample of R. graveolens and E. dracunculoides, respectively. Conclusion: Development of these markers will help in the quality control of herbal drugs and monitoring widespread adulteration of these drugs by pharmaceutical industries and government agencies.

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药用植物石竹及其掺假大戟序列扩增区标记的开发及检测效率

背景:随着草药需求的增加，这些药物的掺假也愈演愈烈，在国内和出口市场上仍然是一个不可缺少的问题。正确鉴别是保证本土草药质量、安全性和有效性的第一步。材料与方法:建立了序列特征扩增区(SCAR)标记，对芦笋及其伪品大戟进行鉴别。随机扩增多态性DNA (RAPD)随后转化为SCAR标记。结果:进行RAPD后，根据所选择的真药和假药的RAPD特异扩增子设计SCAR引物。这些引物分别产生了670 bp和750 bp的SCAR标记。结论:这些标志物的开发将有助于中药质量控制和监测制药行业和政府机构普遍存在的掺假现象。

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Drug Development and Therapeutics

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