IMPACT OF ATRAZINE AND BROMOXYNIL ON THE COLONY FORMING UNITS (CFU) OF SOIL BACTERIA

Syed Majid Rasheed
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Abstract

According to overwhelmingly positive effects throughout time, herbicide treatment has become a crucial component of thriving agricultural output worldwide. However, its detrimental effects on non-target soil microorganisms involved in the nitrogen cycle, nutrient degradation, and organic matter breakdown must be considered. In the current study, the consequences of the two (2) herbicides that are most often used in Pakistan, Atrazine and Bromoxynil, were evaluated on soil bacteria over the course of fifteen consecutive days (exposure period). Recommended field rate of herbicide application was followed (i.e. active ingredient of 6.17 mg for Atrazine and 2.4 mg for Bromoxynil per gm of soil). During the investigations half and double recommended doses of these herbicides were used. Time interval of 5, 10 and 15 days were used to determine the bacterial populations. Statistical analysis of the investigation revealed that the bacterial population did not exhibit any appreciable variations in relation to the exposure duration (p 0.05). The Atrazine application along with the herbicide treatments, reduced the bacterial population during all the tested interval durations with just half of the suggested field rate. The current study reflected that apart from the benefit of herbicides in controlling weeds these chemicals also effect bacterial population that is a serious issue for present and future agriculture. Further, the same study needs to be perform under field conditions to confirm the finding of laboratory results before issuing solid recommendations to the farming community.
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阿特拉津和溴腈对土壤细菌菌落形成单位(cfu)的影响
根据长期以来压倒性的积极影响,除草剂处理已成为全球农业产量蓬勃发展的关键组成部分。然而,它对参与氮循环、养分降解和有机质分解的非目标土壤微生物的不利影响必须加以考虑。在目前的研究中,在连续15天(暴露期)的过程中,评估了巴基斯坦最常用的两种除草剂阿特拉津(Atrazine)和溴霉尼(Bromoxynil)对土壤细菌的影响。遵循推荐的田间除草剂施用量(即每克土壤有效成分为阿特拉津6.17毫克和溴硝腈2.4毫克)。在调查期间,这些除草剂的使用剂量是推荐剂量的一半和两倍。时间间隔为5、10和15 d,测定细菌数量。调查的统计分析显示,细菌种群没有表现出与暴露时间相关的任何明显变化(p 0.05)。在所有测试间隔时间内,阿特拉津与除草剂一起施用,减少了细菌数量,仅为建议田间率的一半。目前的研究表明,除了除草剂在控制杂草方面的好处外,这些化学物质还会影响细菌数量,这对当前和未来的农业来说是一个严重的问题。此外,同样的研究需要在实地条件下进行,以确认实验室结果的发现,然后再向农业社区提出可靠的建议。
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