SSR marker based profiling and diversity analysis of mungbean [Vigna radiata (L.) Wilczek] genotypes

IF 0.8 4区 生物学 Q3 AGRONOMY Legume Research Pub Date : 2019-01-01 DOI:10.18805/LR-3918
S. Suman, B. Rani, V. Sharma, H. Kumar, V. Shahi
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引用次数: 5

Abstract

The evaluation and characterization of germplasm of mungbean are considered to be essential prerequisites for a rational use of its genetic resources. Accordingly in the present study, genetic diversity in 18 mungbean genotypes was assessed using a panel of 40 microsatellite based primer pairs. Out of 40 primers initially tested, only 24 showed distinct polymorphism and consequently only these primers were utilized for the purpose of genome profiling of the entries. Altogether, 183 allelic variants were detected with an average of 4.95 polymorphic fragment per primer. The number of amplified loci varied from 4 (CEDG 15) to a maximum of 12 (CEDG 92 and CEDG 172) with an average of 4.95 polymorphic fragment per primer. The polymorphic information content (PIC) ranged from 0.440 to 0.928 with an average of 0.822 per SSR primer pair. The lowest polymorphism percent was observed for primer CEDGAG001 (16.67) while the primer CEDG 154 gave the maximum polymorphism percent (63.64). The primer pairs CEDG 008, CEDG 068 and CEDG 154 among the total primer pairs were found to be highly informative. Dice's similarity coefficient ranged from 0.03 to 0.58. Dendrogram based on SSR data grouped the mungbean genotypes into seven clusters. The pattern of clustering was also reiterated by the results of principal component analysis (PCA). The SSR primer based analysis allowed unique and unambiguous genotyping of the entries. The genetic diversity observed in the present study could be useful to know the phylogenetic links among the cultivars and for the selection of suitable parents to be further used in breeding programmes and genetic mapping studies.
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基于SSR标记的绿豆(Vigna radiata, L.)遗传分析及多样性分析Wilczek)基因型
绿豆种质资源的评价和鉴定是合理利用绿豆遗传资源的重要前提。因此,本研究利用40对基于微卫星的引物对对18个绿豆基因型进行了遗传多样性评估。在最初测试的40个引物中,只有24个表现出明显的多态性,因此只有这些引物被用于条目的基因组分析。共检测到183个等位变异,平均每个引物有4.95个多态性片段。扩增的位点数量从4个(CEDG 15)到最多12个(CEDG 92和CEDG 172)不等,平均每个引物有4.95个多态性片段。多态性信息含量(PIC)为0.440 ~ 0.928,平均为0.822。引物CEDGAG001多态性率最低(16.67),引物CEDG 154多态性率最高(63.64)。其中引物对CEDG 008、CEDG 068和CEDG 154信息量较大。Dice的相似系数从0.03到0.58不等。基于SSR数据的树图将绿豆基因型划分为7个聚类。主成分分析(PCA)的结果也重申了聚类模式。基于SSR引物的分析允许对条目进行独特和明确的基因分型。本研究所观察到的遗传多样性有助于了解品种之间的系统发育联系,并为选择合适的亲本进一步用于育种计划和遗传作图研究。
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来源期刊
Legume Research
Legume Research AGRONOMY-
CiteScore
1.30
自引率
37.50%
发文量
256
审稿时长
6-12 weeks
期刊介绍: Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.
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