A Comparative Study of Phytochemical and Biochemicals of Micropropagated and Wild Rhazya stricta Decne

Abstract

Wild plants are creation of nature which are source of food, fuel,shelter,fibers,gum,andherbal medicine. Present study was carried out to investigate the biochemicals and phytochemicals of wild and micropropagated Rhazya stricta plant. The present investigation was aimed to promote the benefits of wild plants. For this purpose, in-vitro micropropagation of Rhazya stricta (family Apocynaceae) was successfully achieved. 20% extract was prepared in acetone, ethanol and water solvents from stem, root, fruit and leaf of Rhazya stricta(collected from Jamshoro district) and root, stem and leaves from micropropagated plant which were investigated to analyze the phytochemical and biochemicals. Result showed that highest anti-oxidants were observed from acetone extract of leaves (in-vivo) as 13.60mg/ml, flavonoids form ethanol extract of fruit as 1.98mg/ml, total phenolics from ethanol extract of leaves (in-vivo) as 0.84mg/ml, tannin from acetone extract of stem(in-vitro) as 0.61mg/ml, reducing power from ethanol extract of stem (in-vitro) as 1.82mg/ml, protein from acetone extract of leaves (in-vivo) as 9.91mg/ml, total sugar from acetone extract of leaves (in-vitro) as 8.00mg/ml and reducing sugar from ethanol extract of leaves(in-vitro) as 0.99mg/ml were found from under investigation plants.