Role of Healthy Human Gut Microbiota in the Emergence and Dissemination of Extended-Spectrum \(\beta\)-lactamase-Producing Enterobacteriaceae and Genes Associated with \(\beta\)-lactam Resistance in Community Settings in Abidjan, Côte d'Ivoire

Abstract

Overuse of β-lactam antibiotics in communities in developing countries has transformed healthy human intestinal flora into a reservoir of antibiotic-resistant organisms. The prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in community settings remains undetermined. In order to obtain data on ESBL enterobacteria, 265 stool samples were collected from August 2019 to February 2020 from individuals residing in the urban districts of Abidjan and attending medical consultations at the Institut Pasteur de Côte d'Ivoire. Isolates belonging to family Enterobacteriaceae were isolated on MacConkey and identified using the API 20E galerie and antibiotic susceptibility was determined using Clinical Laboratory Standard Institute disc diffusion method. Detection of extended spectrum β-lactamases (TEM, SHV, GES, PER, VEB, CTXM 1, CTXM 2, CTXM 8 and CTXM 9) was done by simplex and multiplex PCR. The human stools strains consisted of 513 species of Enterobacteria multidrug resistants. Among the 513 strains, 75 (14.6%) of the enterobacterial strains produced ESBLs, while 438 (85.4%) produced high-level cephalosporinases. Enterobacteria producing extended-spectrum β-lactamase we dominated by the species Escherichia coli (46.7%), Klebsiella pneumoniae (17.3%), Enterobacter cloacae (13.3%), Enterobacter aerogenes (6.7%), Proteus mirabilis (6.7%), Klebsiella oxytoca (4%), Proteus vulgaris (2.7%), Citrobacter koseri (1.3%), and Citrobacter freundii (1.3%). Strains were resistant (100%) to antibiotics from beta-lactam family (penicillin with inhibitor, monobactam, cephalosporin) but low level resistant (1,3%) was observed to carbapenem (imipénème, méropénème, Ertapenem). The rate of resistance to quinolones and aminoglycosides were respectively between 22.9% - 43.3% and 7.9-35.1%. The resistance genes TEM, SHV, CTXM 1, CTXM 2, CTXM 8 and CTXM 9 were detected. No GES and PER genes were not detected. The high fecal carriage rate of ESBL-PE associated with genes in community settings of Ivory Coast highlights the risk for transmission and dissemination because healthy people are potential patients on borrowed time.