Optimization of Cu, Hg and Cd removal by Enterobacter cloacae by ferric ammonium citrate precipitation

Advances in Environmental Research Pub Date : 2014-12-25 DOI:10.12989/AER.2014.3.4.283

Rashmi Singh, D. Tipre, S. Dave

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Abstract

Abstract. Iron precipitating organisms play a significant role in the formation of ferric hydroxide precipitate, which acts as strong adsorbent for toxic metal. In this respect four different iron precipitating cultures were isolated from Hutti gold mine surface winze water sample on citrate agar medium. The best isolate was screened out for metal removal study on the basis of fast visual iron precipitation. The selected isolate was identified as Enterobacter sp. based on routine biochemical tests and Biolog GN microplate results and as Enterobacter cloacae subsp. dissolvens by 16S rRNA gene sequence analysis (GenBank accession number EU429448). Influence of medium composition, medium initial pH, the influence of inoculum size, effect of various media and ferric ammonium citrate concentration were studied on metal removal in shake flask experiments. Under the optimized c onditions studied, E . cloacae showed 94 ± 2, 95 ± 2 and 70 ± 2% of cadmium, copper and mercury removal from a simulated waste in shake flask studies. In lab scale column reactor more than 85% of copper and mercury removal was achieved.

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柠檬酸铁铵沉淀法对阴沟肠杆菌去除Cu、Hg和Cd的优化

摘要铁沉淀生物在氢氧化铁沉淀的形成中起着重要作用，氢氧化铁是有毒金属的强吸附剂。在柠檬酸琼脂培养基上，从虎提金矿表面水样中分离出4种不同的铁沉淀培养物。在快速视觉铁沉淀的基础上筛选出最佳的金属去除菌株。经常规生化试验和biologgn微孔板检测，所选分离物鉴定为肠杆菌属和阴沟肠杆菌亚种。通过16S rRNA基因序列分析溶解(GenBank登录号EU429448)。在摇瓶试验中，研究了培养基组成、培养基初始pH、接种量、不同培养基的作用以及柠檬酸铁铵浓度对金属脱除的影响。在优化条件下，E。在摇瓶研究中，泄殖腔对模拟废物中的镉、铜和汞的去除率分别为94±2%、95±2%和70±2%。在实验室规模的塔式反应器中，铜和汞的去除率达到85%以上。

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Advances in Environmental Research

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