Antiproliferative potential of Sargassum binderi Sonder ex J. Agardh mediated biogenic silver/silver chloride nanoparticles on EAC, MCF-7 and HCT116 cells
M. Ahamed, M. M. Rahman, I. Hasan, S. Kabir, A. Asaduzzaman
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引用次数: 0
Abstract
Biosynthesis of Ag/AgCl-NPs from Sargassum binderi extract was confirmed by colour changes and a sharp peak at 416 nm detected by UV-visible spectrophotometer. It was characterized by Transmission Electron Microscopy (TEM), Energy Dispersive X-ray (EDX) spectrophotometer, X-ray powder Diffraction (XRD) and Fourier-Transform Infrared Spectroscopy (FTIR). Mild toxicity of the synthesized Ag/AgCl-NPs was determined by the brine shrimp nauplii cytotoxicity assay. IC 50 values of 19.73, 25.56, and 14.00 𝜇 g/ml showed the growth inhibition of Ehrlich Ascites Carcinoma (EAC), human breast cancer (MCF-7) and human colon cancer (HCT116) cells by Ag/AgCl-NPs, respectively. By analyzing cell morphological study using annexin V and caspase-3 inhibitor, initiation of apoptosis in EAC cells was confirmed. The results were further supported through the expression of several genes, where, p53 and Bax genes became activated and Bcl-X and NF κ B genes were suppressed.
期刊介绍：
Started in 1972, this multi-disciplinary journal publishes full papers and short communications. The Indian Journal of Geo-Marine Sciences, issued monthly, is devoted to the publication of communications relating to various facets of research in (i) Marine sciences including marine engineering and marine pollution; (ii) Climate change & (iii) Geosciences i.e. geology, geography and geophysics. IJMS is a multidisciplinary journal in marine sciences and geosciences. Therefore, research and review papers and book reviews of general significance to marine sciences and geosciences which are written clearly and well organized will be given preference.
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