EKSTRAKSI DAUN PELAWAN (Tristaniopsis merguensis)SEBAGAI ANTIOKSIDAN MENGGUNAKAN MICROWAVE ASSISTED EXTRACTION (MAE)

Sito Enggiwanto, Fusfita Istiqomah, Kasro Daniati, Occa Roanisca, Robby Gus Mahardika
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引用次数: 8

Abstract

Indonesia is a country that produces a wealth of abundant biodiversity, one of which is a plant that is often cultivated the tree of Pelawan (Tristaniopsis merguensis). Pelawan trees are widely spread in Indonesia one of them found in the forests of the Bangka Belitung island. Therefore, this study aims to identify the secondary metabolites and testing antioxidants in leaf of pelawan extract by comparing methods of Microwave Assisted Extraction (MAE) with maceration method. The resistant leaf used in this study was obtained from Sempan, Bangka Belitung. The observation result shows that the use of MAE method can extract with faster time compared with maceration method. So the MAE method is more effective than the maceration method. The using of MAE method for 30 minutes obtained the highest yield percentage of 41.3% in ethanol solvent which is more effective than acetone solvent. Extraction by using 48 hour maceration yields is 28.985%. While the extraction with 30 minute maceration is 13.385%. Testing of antioxidant activity using Microwave Assisted Extraction (MAE) method on acetone solvent has the highest antioxidant activity than ethanol extract that is 9,501 μg/mL. The use of the MAE method is more efficient in extracting the leaves of Pelawan than the maceration method when viewed from the time and antioxidant activity.
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印度尼西亚是一个拥有丰富生物多样性的国家,其中一种植物是经常种植的Pelawan树(tristiopsis merguensis)。白望海树在印度尼西亚广泛分布,其中一种在邦加勿里洞岛的森林中发现。因此,本研究旨在通过微波辅助提取(MAE)和浸渍法的比较,对刺参叶提取物的次生代谢产物进行鉴定和抗氧化剂检测。本研究使用的抗性叶片来自邦卡别里洞的Sempan。观察结果表明,与浸渍法相比,MAE法提取时间更快。因此MAE法比浸渍法更有效。在乙醇溶剂中反应30 min,得率最高,达41.3%,比丙酮溶剂更有效。浸渍48 h提取得率为28.985%。浸渍30 min浸出率为13.385%。丙酮溶剂微波辅助提取法测定其抗氧化活性,其抗氧化活性最高,为9501 μg/mL。从提取时间和抗氧化活性两方面来看,采用MAE法提取海参叶的效率高于浸渍法。
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