Human leukocyte antigen-genotyping according to sex in extrapulmonary tuberculosis patients in Iraq

Abstract

Methods: One-hundred whole blood samples were collected from patients with EPTB (n1⁄450) and healthy control patients (n1⁄450). The sequence-specific oligonucleotide was used for HLA-DRB1 genotyping. Results: The HLA-DRB1 7 (relative risk1⁄44.35) and HLA-DRB1 3 (relative risk1⁄43.20) genotypes were significantly more frequent among patients with EPTB compared with healthy subjects (38 vs. 16%, P1⁄40.002 and 27 vs. 8%, P1⁄40.002, respectively). The total alleles of the HLA-DRB1 were more common among female patients (60%) vs. male patients (40%). From the result of HLA-DRB1 genotypes, most of the females had two alleles, whereas males mostly had one allele. The frequency of the HLA-DRB1 3 and HLA-DRB1 7 was significantly more common in female patients (72 and 62%, respectively), than male patients (28 and 38%, respectively). Moreover, this result was observed for the genotype HLA-DRB1 15.