The effect of Myrtus communis L. extract on nephrolithiasis model in rats.

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Northern Clinics of Istanbul Pub Date : 2024-04-24 eCollection Date: 2024-01-01 DOI:10.14744/nci.2023.09068
Busra Ertas, Dogancan Dorucu, Oznur Gulerturk, Ali Sen, Ozge Cevik, Sule Cetinel, Pinar Eker, Asuman Akgun, Emre Tarik Sener, Goksel Sener
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Abstract

Objective: Nephrolithiasis is a common urological disease that can lead to renal failure. Oxidative stress has been shown to be a contributing factor for nephrolithiasis and many agents have been studied to prevent and treat oxidative stress-related nephrolithiasis and renal damage. Myrtus communis (MC) extract has been shown to be an important antioxidant in different animal models. In this study, MC extract was administered preventively or therapeutically to rats with kidney stones, and its effectiveness was investigated.

Methods: Wistar albino rats were divided into four groups (n=8); control (C), ethylene glycol (EG), EG+preventive MC, and EG+curative MC groups. The nephrolithiasis model was created by adding 0.75% EG to drinking water for 8 weeks. Ultimately, 24-hour urine was collected to measure calcium, citrate, and creatinine levels. After decapitation, kidney tissues were harvested for histological analyses, measurement of osteopontin and 8-hydroxydeoxyguanosine (8-OHdG) levels, and N-acetyl-β-glucosaminidase (NAG), myeloperoxidase (MPO) and caspase-3 activities.

Results: In 24-hour urine samples, calcium, citrate and creatinine levels were decreased in the EG group, while oxalate levels were increased and in treatment groups these parameters returned to control levels. MPO, 8-OHdG, caspase-3 and NAG activity were significantly increased in tissue and these changes were reversed in both MC groups. Histological findings also supported the biochemical parameters.

Conclusion: MC can reduce oxidative stress and histopathological changes in kidney tissues in rat nephrolithiasis model when used as either a preventive or therapeutic agent. If supported with further clinical trials, MC might have clinical implications in preventing oxidative renal cell injury and ultimately kidney stone formation.

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香桃木提取物对大鼠肾结石模型的影响
目的:肾结石是一种常见的泌尿系统疾病,可导致肾功能衰竭。氧化应激已被证明是导致肾结石的一个因素,人们研究了许多药物来预防和治疗与氧化应激相关的肾结石和肾损伤。在不同的动物模型中,桃金娘(MC)提取物已被证明是一种重要的抗氧化剂。本研究对肾结石大鼠进行了MC提取物的预防或治疗,并对其有效性进行了调查:Wistar白化大鼠分为四组(n=8):对照组(C)、乙二醇组(EG)、EG+预防性MC组和EG+治疗性MC组。通过在饮用水中添加 0.75% 的乙二醇,建立肾炎模型,为期 8 周。最终,收集 24 小时尿液以测量钙、柠檬酸盐和肌酐水平。断头后,采集肾组织进行组织学分析,测量骨化素和 8-羟基脱氧鸟苷(8-OHdG)水平,以及 N-乙酰基-β-氨基葡萄糖苷酶(NAG)、髓过氧化物酶(MPO)和 caspase-3 活性:在 24 小时尿样中,EG 组的钙、柠檬酸盐和肌酐水平降低,而草酸盐水平升高,治疗组的这些参数恢复到控制水平。组织中的 MPO、8-OHdG、caspase-3 和 NAG 活性显著增加,这些变化在 MC 组和 EG 组均得到逆转。组织学研究结果也支持生化指标:结论:在大鼠肾结石模型中,MC 作为一种预防或治疗药物可减少氧化应激和肾组织病理变化。如果得到进一步临床试验的支持,MC 在预防氧化性肾细胞损伤和最终肾结石形成方面可能具有临床意义。
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来源期刊
Northern Clinics of Istanbul
Northern Clinics of Istanbul MEDICINE, GENERAL & INTERNAL-
CiteScore
0.40
自引率
0.00%
发文量
48
审稿时长
10 weeks
期刊最新文献
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