In-vitro screening of amylase producing halophilic bacteria isolated from seawater

IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Indian Journal of Geo-Marine Sciences Pub Date : 2022-05-01 DOI:10.56042/ijms.v51i05.65568
S. Abirami, M. Lakshmi, A. Samrot, P. J. J. Cypriyana, S. Saigeetha, G. Narendrakumar
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Abstract

In this study, eight halobacterial strains were isolated from seawater. Among the eight isolates, four strains grew well in nutrient medium with 40 g/100 mL of sodium chloride concentration. Amongst all, HA01 was found to show highest amylase activity than others. Highest amylase producing bacteria, i.e . HA01 was subjected for 16S rRNA sequence based identification and it was found to be Aquabacterium sp. strain AS02. The organism showed optimal amylase production at pH 7 and temperature of 42 °C and NaCl concentration of 3 M. The two substrates (banana peel and potato peel) were used for the amylase production among which banana peel showed that highest amylase activity of 6.12 U/mL than potato peel (4.27 U/mL).
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海水中产淀粉酶嗜盐菌的体外筛选
本研究从海水中分离出8株盐杆菌。8株菌株中,4株在氯化钠浓度为40 g/100 mL的营养培养基中生长良好。其中,HA01的淀粉酶活性最高。产生最高淀粉酶的细菌,即经16S rRNA序列鉴定,HA01为水族细菌(aquabobacterium sp.)菌株AS02。在pH为7、温度为42°C、NaCl浓度为3 m的条件下,该菌产生淀粉酶的最佳条件为香蕉皮和马铃薯皮,其中香蕉皮的淀粉酶活性最高,为6.12 U/mL,马铃薯皮为4.27 U/mL。
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来源期刊
CiteScore
1.50
自引率
0.00%
发文量
0
审稿时长
1.7 months
期刊介绍: Started in 1972, this multi-disciplinary journal publishes full papers and short communications. The Indian Journal of Geo-Marine Sciences, issued monthly, is devoted to the publication of communications relating to various facets of research in (i) Marine sciences including marine engineering and marine pollution; (ii) Climate change & (iii) Geosciences i.e. geology, geography and geophysics. IJMS is a multidisciplinary journal in marine sciences and geosciences. Therefore, research and review papers and book reviews of general significance to marine sciences and geosciences which are written clearly and well organized will be given preference.
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