Synergistic Effects of Fructooligosaccharide and Bacillus subtilis Dietary Supplementation on Growth, Innate Immune Responses, and Protection against Streptococcus agalactiae in Juvenile Nile Tilapia (Oreochromis niloticus)

IF 0.6 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Chiang Mai Journal of Science Pub Date : 2023-09-29 DOI:10.12982/cmjs.2023.050
Arporn Panase, M. Thirabunyanon, J. Promya, Dušan Palić, C. Chitmanat
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Abstract

T he primary objective of this study was to examine the impact of combining fructooligosaccharide (FOS) and Bacillus subtilis as the synbiotic effect on the growth, innate immunity functions, and protection against Streptococcus agalactiae in tilapia (Oreochromis niloticus). During the 56-day feeding trial, tilapia with an average weight of 24.5 ± 1.6 g were fed four different types of diets, including a control diet (G1) and three other diets (G2, G3, G4) supplemented with varying levels of fructooligosaccharides (FOS) and Bacillus subtilis at doses of 1 g FOS /kg feed + 1x109 CFU B. subtilis /g feed (G2), 3 g FOS /kg feed + 3x109 CFU B. subtilis /g feed (G3), and 5 g FOS /kg feed + 5x109 CFU B. subtilis /g feed (G4), respectively. Quantitative Reverse Transcription PCR (qRT-PCR) was utilized to quantify the expression levels of immune-related genes in the liver tissue of tilapia at the end of the feeding trial. The immune-related genes evaluated comprised complement C3, IL-1β, IL-8, TNF-α, IFN-γ, and hsp70. Following the experimental feeding, tilapia were intraperitoneally injected with S. agalactiae. The results showed that the synbiotic feeding did not significantly impact the growth performances or survival of the fish (p > 0.05). However, tilapia fed with G3 and G4 diets exhibited significantly higher lysozyme and respiratory burst activities (p < 0.05). Tilapia fed with synbiotic additives showed significant modulation of immune-related genes and slightly higher survival rates after the challenge with S. agalactiae compared to the control group. These findings suggest that synbiotic supplementation may enhance the innate immune responses of tilapia against S. agalactiae infection.
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低聚果糖和枯草芽孢杆菌对尼罗罗非鱼幼鱼生长、先天免疫反应及抗无乳链球菌的协同作用
本研究的主要目的是研究低聚果糖(FOS)与枯草芽孢杆菌(Bacillus subtilis)联合使用对罗非鱼(Oreochromis niloticus)生长、先天免疫功能和抗无乳链球菌感染的影响。56-day喂养试验期间,罗非鱼,平均24.5±1.6克的重量被喂以四种不同的饮食,包括控制饮食(G1)和三个其他饮食(G2、G3、G4)与不同程度的补充fructooligosaccharides (FOS)提交和枯草芽孢杆菌在剂量的1 g”丛书/公斤饲料+ 1 x109 CFU枯草芽孢杆菌/ g饲料(G2), 3 g安全系数/公斤饲料+ 3 x109 CFU枯草芽孢杆菌/ g饲料(G3)和5 g”丛书/公斤饲料+ 5 x109 CFU枯草芽孢杆菌/ g饲料(G4),分别。采用定量反转录PCR (qRT-PCR)技术,定量测定罗非鱼饲喂结束后肝脏组织中免疫相关基因的表达水平。评估的免疫相关基因包括补体C3、IL-1β、IL-8、TNF-α、IFN-γ和hsp70。试验饲养后,向罗非鱼腹腔注射无乳链球菌。结果表明,合成饲料对鱼的生长性能和成活率无显著影响(p < 0.05)。而饲喂G3和G4的罗非鱼溶菌酶和呼吸爆发活性显著高于对照组(p < 0.05)。与对照组相比,添加了合成添加剂的罗非鱼在无乳链球菌攻毒后表现出免疫相关基因的显著调节和略高的存活率。这些发现表明,添加合成菌可以增强罗非鱼对无乳链球菌感染的先天免疫反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chiang Mai Journal of Science
Chiang Mai Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.00
自引率
25.00%
发文量
103
审稿时长
3 months
期刊介绍: The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.
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