POTENSI STREPTOMYCES ENDOFIT RIMPANG KENCUR DAN DAUN SIRIH MERAH SEBAGAI ANTITUBERKULOSIS PADA MYCOBACTERIUM TUBERCULOSIS GALUR SENSITIF

JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER Pub Date : 2021-01-28 DOI:10.53864/jifakfar.v3i1.38

Siti Nur Azizah, Mikhania Cristiningtyas Eryani

{"title":"POTENSI STREPTOMYCES ENDOFIT RIMPANG KENCUR DAN DAUN SIRIH MERAH SEBAGAI ANTITUBERKULOSIS PADA MYCOBACTERIUM TUBERCULOSIS GALUR SENSITIF","authors":"Siti Nur Azizah, Mikhania Cristiningtyas Eryani","doi":"10.53864/jifakfar.v3i1.38","DOIUrl":null,"url":null,"abstract":"The objective of this research was to obtain Endophitic Streptomyces from some medical plants which have antituberculosis effect and also to test its antitubercullosis activity againts sensitive stain of Mycobacterium tuberculosis. Medicinal plants used in this study were Kaemferia galanga, Hibiscus sabdariffa and Piper cronatum. Streptomyces was isolated using two different media namely Humic Acid Vitamin (HV) agar and HV with plant extract, and purified on Yeast Malt Agar (YMA) medium. Morphological characterization of Streptomyces obtained was observed including aerial mycelium and substrate mycelium, pigmentation, and colony surface. A total of 5 endophitic Streptomyces genus were obtained from medicinal plant. Isolates K.J3A, K.J3B, K.J4 and K.J11 from Kaemferia galanga radix, S.MJ1 from Piper cronatum folium. Streptomyces was not found from Hibiscus sabdariffa calyx. Each isolate had variety of characteristics which could be seen in aerial mass color, substrate mass color, and pigmentation. Antitubercullosis activity showed that total of 5 endophitic Streptomyces were not capable to inhibit the growth sensitive stain of M. tubercullosis.Keywods: Antituberculosis, endophitic actinomycetes, Kaemferia galanga, Hibiscus sabdariffa","PeriodicalId":17737,"journal":{"name":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","volume":"84 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.53864/jifakfar.v3i1.38","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 1

Abstract

The objective of this research was to obtain Endophitic Streptomyces from some medical plants which have antituberculosis effect and also to test its antitubercullosis activity againts sensitive stain of Mycobacterium tuberculosis. Medicinal plants used in this study were Kaemferia galanga, Hibiscus sabdariffa and Piper cronatum. Streptomyces was isolated using two different media namely Humic Acid Vitamin (HV) agar and HV with plant extract, and purified on Yeast Malt Agar (YMA) medium. Morphological characterization of Streptomyces obtained was observed including aerial mycelium and substrate mycelium, pigmentation, and colony surface. A total of 5 endophitic Streptomyces genus were obtained from medicinal plant. Isolates K.J3A, K.J3B, K.J4 and K.J11 from Kaemferia galanga radix, S.MJ1 from Piper cronatum folium. Streptomyces was not found from Hibiscus sabdariffa calyx. Each isolate had variety of characteristics which could be seen in aerial mass color, substrate mass color, and pigmentation. Antitubercullosis activity showed that total of 5 endophitic Streptomyces were not capable to inhibit the growth sensitive stain of M. tubercullosis.Keywods: Antituberculosis, endophitic actinomycetes, Kaemferia galanga, Hibiscus sabdariffa

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

本研究的目的是从一些药用植物中获得具有抗结核作用的内生链霉菌，并检测其对结核分枝杆菌敏感菌的抗结核活性。本研究使用的药用植物有山柰、芙蓉和龙脑草。采用腐植酸维生素(HV)琼脂和含植物提取物的HV两种培养基分离链霉菌，在酵母麦芽琼脂(YMA)培养基上纯化链霉菌。观察所得链霉菌的形态特征，包括气生菌丝和底生菌丝、色素沉着和菌落表面。从药用植物中分离到5个内生链霉菌属。高良姜中分离到K.J3A、K.J3B、K.J4和K.J11，牛蒡叶中分离到S.MJ1。在芙蓉花萼中未发现链霉菌。每个分离物都具有多种特征，可以在空中团块颜色，底物团块颜色和色素沉着中看到。抗结核活性表明，共有5种内生链霉菌不能抑制结核分枝杆菌的生长敏感染色。关键词:抗结核，内生放线菌，高良姜山柰，芙蓉

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER

自引率

0.00%

发文量