{"title":"HPLC Detectors, Their Types and Use: A Review","authors":"A. Sunil","doi":"10.19080/OMCIJ.2018.06.555700","DOIUrl":null,"url":null,"abstract":"The most powerful technique to determine quantitatively and separate the mixture of composition in today’s modern chemistry is Chromatography especially High Performance Liquid Chromatography or High Pressure Liquid Chromatography [1]. HPLC works on the principle of Affinity chromatography. The solution of the sample is injected into a column of a porous material (stationary phase) and a liquid (mobile phase) is pumped at high pressure through the column. The mixture on travelling through the stationary phase splits into its constituents and the component with high affinity for stationary phase travels late whereas one with less affinity elutes fast. This is also based partition coefficient of the material [2]. Abstract","PeriodicalId":19547,"journal":{"name":"Organic & Medicinal Chemistry International Journal","volume":"52 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Organic & Medicinal Chemistry International Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.19080/OMCIJ.2018.06.555700","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
The most powerful technique to determine quantitatively and separate the mixture of composition in today’s modern chemistry is Chromatography especially High Performance Liquid Chromatography or High Pressure Liquid Chromatography [1]. HPLC works on the principle of Affinity chromatography. The solution of the sample is injected into a column of a porous material (stationary phase) and a liquid (mobile phase) is pumped at high pressure through the column. The mixture on travelling through the stationary phase splits into its constituents and the component with high affinity for stationary phase travels late whereas one with less affinity elutes fast. This is also based partition coefficient of the material [2]. Abstract
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