Anamnestic response in rabbit blood leucocyte cultures. Autoradiographic analysis of proteins synthesized in the cells and liberated to the culture medium.

Abstract

The reported experiments show that while α- and β-globulins are formed in the cultured leucocytes in sheep red cell stimulated as well as non-stimulated cultures containing primed as well as non-primed cells, immunoglobulins mainly of γG type are formed and liberated into the culture medium following antigen stimulation of primed cells. The amount of newly synthesized immunoglobulin of γG type was found to be correlated to the agglutinin titres. De novo synthesis of γM was also found, but only in minute amounts and without any close relation to culture time or agglutinin titres.