Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02388.X
S. Rosendal, Thomsen Ac
The concentration and persistence of M. arthritidis in obstructed and unobstructed kidneys of rats after intracardial inoculation has been investigated. Inoculation of 108 c.f.u. M. arthritidis, giving a deposit of 104 c.f.u./g kidney tissue, depressed the resistance against E. coli so that secondary intravenous inoculation of 106 or 107 of E. coli would be followed by severe acute pyelonephritis. These doses of E. coli could not provoke inflammation if given without primary mycoplasma inoculation.
{"title":"Mycoplasmosis: experimental pyelonephritis in rats; Demonstration of antibody in urine and serum.","authors":"S. Rosendal, Thomsen Ac","doi":"10.1111/J.1699-0463.1974.TB02388.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02388.X","url":null,"abstract":"The concentration and persistence of M. arthritidis in obstructed and unobstructed kidneys of rats after intracardial inoculation has been investigated. Inoculation of 108 c.f.u. M. arthritidis, giving a deposit of 104 c.f.u./g kidney tissue, depressed the resistance against E. coli so that secondary intravenous inoculation of 106 or 107 of E. coli would be followed by severe acute pyelonephritis. These doses of E. coli could not provoke inflammation if given without primary mycoplasma inoculation.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"5 1","pages":"895-898"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75306969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04308.X
A. Tärnvik
Lymphocytes were isolated from human blood by a simple procedure combining nylon fibre filtration and centrifugation in colloidal silica-polyvinylpyrrolidone. In the preparation obtained 99.5 ± 0.6 per cent of the leukocytes were lymphocytes; it contained 0–202, mostly less than 20, platelets, and 0–1.5, mostly 0, RBC per 100 leukocytes. The lymphocyte recovery was 13.3 ± 3.3 per cent. In the trypan blue test 2.2 ± 3.0 per cent of the cells were stained.
{"title":"Isolation of lymphocytes from blood. A procedure combining nylon fibre filtration and differential centrifugation.","authors":"A. Tärnvik","doi":"10.1111/J.1699-0463.1970.TB04308.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04308.X","url":null,"abstract":"Lymphocytes were isolated from human blood by a simple procedure combining nylon fibre filtration and centrifugation in colloidal silica-polyvinylpyrrolidone. In the preparation obtained 99.5 ± 0.6 per cent of the leukocytes were lymphocytes; it contained 0–202, mostly less than 20, platelets, and 0–1.5, mostly 0, RBC per 100 leukocytes. The lymphocyte recovery was 13.3 ± 3.3 per cent. In the trypan blue test 2.2 ± 3.0 per cent of the cells were stained.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"115 1","pages":"311-6"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75481090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02342.X
Harald ørjasæter
Perchloric acid extracts (PCA) of cancer tissues, normal and inflammatory tissues, normal sera, urines and salivas were studied to detect carcinoembryonic antigens (CEA) and cross-reacting antigens. The extracts were tested in high concentration by immunodiffusion methods in agarose gel. Substances which showed complete identity with CEA were found in apparently normal tissue, saliva and urine and in inflammatory tissue as well as in primary lung cancer. In addition, material which cross-reacted with CEA was found in all the samples tested in adequate concentrations, including serum, saliva and urine. Both CEA and the non-specific cross-reacting antigens (NCA) were found in samples of purified blood group substance A and B and in saliva from secretor and non-secretor. An α-protein was found in PCA extracts of normal and cancer tissue, normal serum and some urines. Some preliminary observations are reported on the α-protein which seems to share antigenic determinants with the βE-protein possessing NCA determinants.
{"title":"Demonstration of carcinoembryonic antigens (CEA), nonspecific cross-reacting antigens (NCA) and an associated alpha protein in normal human tissues and fluids by immunodiffusion techniques.","authors":"Harald ørjasæter","doi":"10.1111/J.1699-0463.1974.TB02342.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02342.X","url":null,"abstract":"Perchloric acid extracts (PCA) of cancer tissues, normal and inflammatory tissues, normal sera, urines and salivas were studied to detect carcinoembryonic antigens (CEA) and cross-reacting antigens. The extracts were tested in high concentration by immunodiffusion methods in agarose gel. Substances which showed complete identity with CEA were found in apparently normal tissue, saliva and urine and in inflammatory tissue as well as in primary lung cancer. In addition, material which cross-reacted with CEA was found in all the samples tested in adequate concentrations, including serum, saliva and urine. Both CEA and the non-specific cross-reacting antigens (NCA) were found in samples of purified blood group substance A and B and in saliva from secretor and non-secretor. An α-protein was found in PCA extracts of normal and cancer tissue, normal serum and some urines. Some preliminary observations are reported on the α-protein which seems to share antigenic determinants with the βE-protein possessing NCA determinants.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"2 1","pages":"387-395"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72615739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/j.1699-0463.1974.tb02373.x
E. Jantzen, K. Bryn, K. Bøvre
{"title":"Gas chromatography of bacterial whole cell methanolysates; IV. A procedure for fractionation and identification of fatty acids and monosaccharides of cellular structures.","authors":"E. Jantzen, K. Bryn, K. Bøvre","doi":"10.1111/j.1699-0463.1974.tb02373.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1974.tb02373.x","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"65 1","pages":"753-766"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85051519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02344.X
F. Hardt, M. H. Claësson
Amyloidosis was induced by repeated casein injections in a strain of mice with congenital aplasia of the thymus (nu/nu-C3H). The development of amyloidosis in this strain was delayed compared with the development of amyloidosis in the strain of control mice C3H. Spleen grafts from casein sensitized non-amyloidotic “nude” and C3H mice were transferred to mice from both strains. Only grafts from casein-sensitized C3H mice could accelerate amyloid formation in recipients, whereas grafts from the casein-sensitized “nude” mice had no such effect. Antibodies to casein could be detected neither in the donor mice nor in the recipients. The observed acceleration could be due to a transfer of casein stimulated T-lymphocytes. Spleen cells from amyloidotic “nude” and C3H mice were transferred to both strains. Amyloid formation occurred only in recipients belonging to the C3H strain. As amyloid formation in the recipients—in this transfer model—is dependent on heavy cytotoxic treatment, it seems unlikely that amyloid formation in the recipients is due to immune reactions elicited by donor or recipient lymphoid cells. The reason why amyloidosis cannot be transferred by spleen cells to nude mice could be due to a poorer trapping of donor spleen cells in the nude spleens than in the normal C3H spleens.
{"title":"Casein-induced amyloidosis in the nude mouse. I. Acceleration of amyloidosis in recipients of spleen grafts from casein-sensitized donor mice. II. Transfer of amyloidosis by spleen cells.","authors":"F. Hardt, M. H. Claësson","doi":"10.1111/J.1699-0463.1974.TB02344.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02344.X","url":null,"abstract":"Amyloidosis was induced by repeated casein injections in a strain of mice with congenital aplasia of the thymus (nu/nu-C3H). The development of amyloidosis in this strain was delayed compared with the development of amyloidosis in the strain of control mice C3H. Spleen grafts from casein sensitized non-amyloidotic “nude” and C3H mice were transferred to mice from both strains. Only grafts from casein-sensitized C3H mice could accelerate amyloid formation in recipients, whereas grafts from the casein-sensitized “nude” mice had no such effect. Antibodies to casein could be detected neither in the donor mice nor in the recipients. The observed acceleration could be due to a transfer of casein stimulated T-lymphocytes. Spleen cells from amyloidotic “nude” and C3H mice were transferred to both strains. Amyloid formation occurred only in recipients belonging to the C3H strain. As amyloid formation in the recipients—in this transfer model—is dependent on heavy cytotoxic treatment, it seems unlikely that amyloid formation in the recipients is due to immune reactions elicited by donor or recipient lymphoid cells. The reason why amyloidosis cannot be transferred by spleen cells to nude mice could be due to a poorer trapping of donor spleen cells in the nude spleens than in the normal C3H spleens.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"14 1","pages":"403-8"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76809102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04349.X
G. Alm
{"title":"In vitro studies of chicken lymphoid cells. 1. Phytohaemagglutinin induced DNA, RNA and protein synthesis in spleen cells from control-irradiated and bursectomized-irradiated chickens.","authors":"G. Alm","doi":"10.1111/J.1699-0463.1970.TB04349.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04349.X","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"70 1","pages":"632-40"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76190263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04369.X
K. Bøvre
By diagnostic application of genetic transformation, the occurrence of oxidase positive bacteria in the human nose cavity was examined. Neisseria catarrhalis and Moraxella nonliqucfaciens were the predominating organisms, being equally frequent and representing 87.3 per cent of 111 oxidase positive isolates. In part of the study, comprising 130 patients, N.catarrhalis was isolated from 16.2 per cent and M. nonliquefaciens from 17.7 per cent of the nose specimens. M. liquefaciens and M. osloensis were isolated only occasionally, whereas the two species M. phenylpyrouvica and M. kingii were not encountered. Ten per cent of the isolates belonged to genus Neisseria in the strict sense. An ecological distinction is thereby indicated between rodshaped moraxellae (e.g., M. nonliquefaciens) and “false neisseriae” (e.g., N. catarrhalis) on the one hand and the “true neisseriae” on the other, with only the former groups having an important habitat in the nose. N. elongata, a rodshaped member of genus Neisseria, was not observed in this location. About 80 per cent of the N. catarrhalis and M. nonliquefaciens strains were competent in genetic transformation.
{"title":"Oxidase positive bacteria in the human nose incidence and species distribution, as diagnosed by genetic transformation.","authors":"K. Bøvre","doi":"10.1111/J.1699-0463.1970.TB04369.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04369.X","url":null,"abstract":"By diagnostic application of genetic transformation, the occurrence of oxidase positive bacteria in the human nose cavity was examined. Neisseria catarrhalis and Moraxella nonliqucfaciens were the predominating organisms, being equally frequent and representing 87.3 per cent of 111 oxidase positive isolates. In part of the study, comprising 130 patients, N.catarrhalis was isolated from 16.2 per cent and M. nonliquefaciens from 17.7 per cent of the nose specimens. M. liquefaciens and M. osloensis were isolated only occasionally, whereas the two species M. phenylpyrouvica and M. kingii were not encountered. Ten per cent of the isolates belonged to genus Neisseria in the strict sense. An ecological distinction is thereby indicated between rodshaped moraxellae (e.g., M. nonliquefaciens) and “false neisseriae” (e.g., N. catarrhalis) on the one hand and the “true neisseriae” on the other, with only the former groups having an important habitat in the nose. N. elongata, a rodshaped member of genus Neisseria, was not observed in this location. About 80 per cent of the N. catarrhalis and M. nonliquefaciens strains were competent in genetic transformation.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"5 1","pages":"780-4"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77003753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02305.X
S. T. Jørgensen
{"title":"Elimination of antibiotic resistance factors from Escherichia coli exposed to anthelmintics.","authors":"S. T. Jørgensen","doi":"10.1111/J.1699-0463.1974.TB02305.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02305.X","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"85 1","pages":"143-144"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78186749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02284.X
E. Korpinen, J. Uoti
Three types of biological methods were compared as detectors of stachybotrys toxin. The seven preparations tested represented “crude” or partially purified toxins produced by different strains of Stachybotrys alternans and toxic fractions obtained by partial purification of the product of one strain. The order of sensitivity of the methods was almost invariably: the mouse fibroblast test most sensitive, the rabbit skin test next, and the brine shrimp (Artemia salina) test last. A conspicuous variation by preparation in relative sensitivity of the mouse fibroblast test and the rabbit skin test was revealed, however. The variation ranged from a sensitivity of the rabbit skin test approximately 2.5 times higher than that in the mouse fibroblast tests to the other limit when the mouse fibroblast test was 80 times more sensitive than the rabbit skin test. The variation is interpreted to provide the first experimental proof of heterogeneity in biological effect based on chemical differences among the compounds constituting “stachybotrys toxin”. The toxic fractions obtained by purification of two crude preparations and identified as toxic by the mouse fibroblast test showed different patterns of distribution of toxicity. The results demonstrate that qualitative differences may exist in the chemical structure of the population of toxic compounds of “stachybotrys toxin” produced by different strains of S. alternans.
{"title":"Studies on Stachybotrys alternans. II. Occurrence, morphology and toxigenicity.","authors":"E. Korpinen, J. Uoti","doi":"10.1111/J.1699-0463.1974.TB02284.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02284.X","url":null,"abstract":"Three types of biological methods were compared as detectors of stachybotrys toxin. The seven preparations tested represented “crude” or partially purified toxins produced by different strains of Stachybotrys alternans and toxic fractions obtained by partial purification of the product of one strain. The order of sensitivity of the methods was almost invariably: the mouse fibroblast test most sensitive, the rabbit skin test next, and the brine shrimp (Artemia salina) test last. A conspicuous variation by preparation in relative sensitivity of the mouse fibroblast test and the rabbit skin test was revealed, however. The variation ranged from a sensitivity of the rabbit skin test approximately 2.5 times higher than that in the mouse fibroblast tests to the other limit when the mouse fibroblast test was 80 times more sensitive than the rabbit skin test. The variation is interpreted to provide the first experimental proof of heterogeneity in biological effect based on chemical differences among the compounds constituting “stachybotrys toxin”. The toxic fractions obtained by purification of two crude preparations and identified as toxic by the mouse fibroblast test showed different patterns of distribution of toxicity. The results demonstrate that qualitative differences may exist in the chemical structure of the population of toxic compounds of “stachybotrys toxin” produced by different strains of S. alternans.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"47 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73192257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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