Differential expression profiling of defense related genes for Leaf Curl Virus (ChiLCV) in resistant and susceptible genotypes of Chiili

IF 1 4区 生物学 Q3 PLANT SCIENCES Indian Journal of Genetics and Plant Breeding Pub Date : 2020-09-22 DOI:10.31742/IJGPB.80.3.10
M. Mangal, A. Srivastava, S. Mirajkar, Khushbu B. Singh, V. Solanki, B. Mandal, P. Kalia
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引用次数: 4

Abstract

The present investigation was undertaken to study the expression of eight defense related genes in leaf curl resistant line DLS-Sel-10 and susceptible line Phule Mukta after different days post inoculation (dpi) with viruliferous white flies to understand their role in resistance to chilli leaf curl virus (ChiLCV). The expression level of Ca PPO, Ca AsPer, Ca ATP/ADP and CaTopoII was observed to be higher in resistant genotype DLS-Sel-10 than the susceptible Phule Mukta at all the time points studied. Expression of CaNBS-LRR increased up to 12 dpi while that of Ca Thionin, and Ca SKP1 increased up to 24 dpi in the resistant line, thereafter it started declining. The CaSPI expression did not show any specific pattern in both the test plants. The heat map clustered all the genes under study into two major clusters based on their expression profiles, one comprising CaAsPer, Ca-Thionin, CaATP/ADP transporter, CaPPO and CaTopoII while other group comprised CaSKPI, Ca NBS and CaSPI. The challenge inoculation of the test genotypes also revealed that viral titre increased at a much slower rate in DLS-Sel-10 than Phule Mukta, suggesting thereby that DLS-Sel-10 is resisting the accumulation of ChiLCV and has a more active defense machinery than Phule Mukta.
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辣椒卷曲叶病毒防御相关基因在抗性和易感基因型中的差异表达谱
研究了辣椒卷曲叶抗性品系DLS-Sel-10和辣椒卷曲叶敏感品系Phule Mukta在不同接种日(dpi)后8个防御相关基因的表达,以了解它们在辣椒卷曲叶病毒(ChiLCV)抗性中的作用。在研究的所有时间点上,抗性基因型DLS-Sel-10的Ca PPO、Ca AsPer、Ca ATP/ADP和CaTopoII的表达水平均高于敏感基因型。在抗性品系中,CaNBS-LRR的表达量最高可达12 dpi, Ca Thionin和Ca SKP1的表达量最高可达24 dpi,之后开始下降。CaSPI的表达在两种植物中均未表现出特定的模式。热图将研究的所有基因根据其表达谱分成两大簇,一组包括CaAsPer、Ca- thionin、CaATP/ADP转运体、CaPPO和CaTopoII,另一组包括CaSKPI、Ca NBS和CaSPI。试验基因型的攻毒接种也显示,与Phule Mukta相比,DLS-Sel-10的病毒滴度上升速度要慢得多,这表明DLS-Sel-10能够抵抗ChiLCV的积累,并且具有比Phule Mukta更积极的防御机制。
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来源期刊
CiteScore
1.80
自引率
10.00%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.
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