Green synthesis of silver nanoparticles using Manihot esculenta leaves against Aedes aegypti and Culex quinquefasciatus

Abstract

To investigate the silver nanoparticles synthesis using leaves aqueous extract of Manihot esculenta against two important mosquito species, Aedes aegypti and Culex quinquefasciatus. The synthesized silver nanoparticles were monitored by UV–vis spectrophotometer and further characterized by XRD, FESEM and HRTEM. Aqueous extract of M. esculenta appeared to be effective against A. aegypti (LC₅₀ = 66.14 mg/mL; LC₉₀ = 172.41 mg/mL) and C. quinquefasciatus (LC₅₀ = 61.60 mg/mL; LC₉₀ = 184.80 mg/mL). The aqueous silver nitrate 1 Mm solution tested was third instar of A. aegypti and C. quinquefasciatus (LC₅₀ = 76.96 mg/mL; LC₉₀ = 230.88 mg/mL and LC₅₀ = 84.06 mg/L; LC₉₀ = 252.78 mg/mL). The 0.5 mM synthesized Ag PNs against A. aegypti (LC₅₀ = 4.53 mg/mL; LC₉₀ = 13.59 mg/mL); C. quinquefasciatus (LC₅₀ = 3.46 mg/mL; LC₉₀ = 10.38 mg/mL). The 1 mM silver solution synthesized Ag NPs tested were A. aegypti (LC₅₀ = 3.08 mg/mL; LC₉₀ = 9.84 mg/mL) C. quinquefasciatus (LC₅₀ = 3.21 mg/mL; LC₉₀ = 11.24 mg/mL). The control showed nil mortality in the concurrent assay. This is a perfect ecological and inexpensive approach for the control of mosquito larvae.