Regulatory role of peroxinitrite (ONOO-) for oxidative damage to human Red Blood Cells (hRBC)

Abstract

Monitoring of oxidative stress to human red blood cells (hRBC) is scientifically important for any kind of disease etiology or even during of any drug therapies however the comparative toxicity of reactive species in hRBC in term of hemolysis was not yet established. In this context the present study highlighted the different oxidative assay induced either by superoxide (O 2 - )or by hydrogen peroxide (H 2 O 2 )or by nitric oxide (NO) or by peroxinitrite (ONOO - )using different biochemical reactions in isolated hRBC cells. The oxidative hemolysis was significantly ( p 0.05). The induction of oxidative stress by H 2 O 2 was less noticeable may be due to catalase over expression. Therefore, monitoring of ONOO - and reducing oxidative stress should be considered during evaluation of diseases etiology or any kind of drug therapies.