Using correlative microscopy for studying and treatment of Mycoplasma infections of the ophtalmic mucosa

Abstract

The study consists of 21 patients, divided into 3 groups, allergic, not-allergic, and alleged allergic, chosen on clinical history and manifestations shown at the irst visit; the treatment of 21 patients was carried out over a period of 18 months. After the compilation of the ritual consent in matter of privacy to the execution of the investigations, follows the examination with topcon biomicroscope. The Biomicroscope vision allows a primary diagnosis, where we have observed 2 allergic conjunctivitis on the allergic group, 3 dermatoblefaro-conjunctivitis, 1 keratoconjunctivitis and a suppurative calazosis in according to the history; in the group of alleged allergic, we found 3 blepharitis/ conjunctivitis, 2 dermatoblefaro-conjiunctivitis of which 1 with punctate keratitis, 1 blepharitis and 1 conjunctivitis; in the last group (third one non-allergic patients) we described, in according with clinical history and signs, 1 dermatoblefaroconjiunctivitis (on type II diabetic abitus), 3 ulcers of which a cogan-like, 2 conjunctivitis and 1 scleritis. Patients are given a tear drop at the level of the tarsal conjunctiva thanks to a special spatula, this secretion is transferred onto a 13 mm round slide for reading in SEM and on a polarized and colored slide according to the Pappernheim panoptic (MaygrunwaldGiemsa) for reading in optical microscopy; in addition, photos are taken of the biomicroscope with and without luorescein to document the status quo of patients before therapy.