Antioxidant mediated antiulcer effect of Eupatorium triplinerve Vahl against acetic acid induced ulcerative colitis in mice

Abstract

Purpose

Ulcerative colitis (UC) is associated with tainted neutrophil infiltration, deregulated pro-inflammatory mediators, characterized by severe oxidative stress of the intestine. In the present study, an effort was made to evaluate the effect of methanolic fractions of Eupatorium triplinerve (E. triplinerve) (ET) on acetic acid induced ulcerative colitis in male adult mice.

Methods

Colitis in mice was induced with 3.0% acetic acid (v/v) in saline via rectal route. Pre-intervention with E. triplinerve extract (100 mg and 200 mg kg⁻¹ body weight, oral) and reference drug ranitidine (50 mg kg⁻¹ body weight used as reference, oral) 4 days before induction of colitis and was extended up to 8 days.

Results

The phase of inflammation before E. triplinerve extract pre-treatment significantly showed attenuated macroscopic damage, argyrophilic nuclear organization regions (AgNORs) count and histological changes. Similarly, extract also effectively detracts the activity of both Myeloperoxidase (MPO) and Malondialdehyde (MDA) levels by enhancing the cellular antioxidant enzyme levels (Glutathione-s-transferase [GST], Glutathione peroxidise [GPx] and Catalase [CAT]) at the site of ulceration.

Conclusions

The E. triplinerve based therapy resolved that some constituents in extract have an antiulcer effect against UC at colon specific area through its inviolable radical scavenging activity.