Aldose reductase and advanced glycation end products formation inhibitory activity of standardized extracts of Picrorhiza kurroa (Royle ex benth) and Hibiscus rosa-sinensis (Linn.)

A. Santhosh, C. Veeresham, A. R. Rao
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引用次数: 2

Abstract

Objective: The objectives were to study the in-vitro and in-vivo aldose reductase and in-vitro advanced glycation end products formation inhibitory activities of the standardized extracts of Picrorhiza kurroa roots and Hibiscus rosa-sinensis flowers. Methods: In-vitro Aldose reductase inhibitory activity was studied by using isolated rat lens and kidney Aldose reductase by UV-Visible spectro photo metric method by using Quercetin as reference compound. In-vivo Aldose reductase inhibitory activity was evaluated by using experimental rat models of galactosemia and the final lens galactitol was evaluated by High performance liquid chromatography and Gas chromatography methods by using Quercetin as reference compound. In-vitro advanced glycation end products formation inhibitory activity was estimated by using laboratory test reaction with protein and sugars by spectro fluorimetric method by sung aminoguanidine as reference compound. Statistical analysis of the results was done by using Analysis of the variance method. Results: The plant extracts were found to possess significant aldose reductase and advanced glycation end products formation inhibitory activity. Conclusions: More study is required for isolation and characterization of the chief chemical constituents responsible for the biological activity of the plant extracts.
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苦参和芙蓉标准化提取物醛糖还原酶和晚期糖基化终产物形成抑制活性的研究
目的:研究芍药根和芙蓉花标准提取物体外和体内醛糖还原酶活性及体外晚期糖基化终产物形成抑制活性。方法:以槲皮素为对照物,采用紫外可见分光光度法,以离体大鼠晶状体和肾脏醛糖还原酶为对照物,研究醛糖还原酶的体外抑制活性。采用实验性半乳糖血症大鼠模型评价其体内醛糖还原酶抑制活性,以槲皮素为参比化合物,采用高效液相色谱法和气相色谱法评价最终晶状体半乳糖醇。以sung氨基胍为参比化合物,采用荧光光谱法与蛋白质和糖进行实验反应,评估了体外晚期糖基化终产物形成的抑制活性。采用方差分析法对结果进行统计分析。结果:该植物提取物具有显著的醛糖还原酶和晚期糖基化终产物形成抑制活性。结论:对该植物提取物生物活性的主要化学成分的分离和鉴定有待进一步研究。
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