Production of specific antiserum against aflatoxin g1 and determination of its efficiency to detect the toxin in wheat and barley grains

Abstract

The present study was carried out to produce antiserum for aflatoxin G1 (AFG1) and to use indirect ELISA to detect Aflatoxin in grain samples. Antiserum of AFG1 was produced in New Zealand white rabbits. The results of indirect ELISA showed that the second collection (after 7 days) was the best that contained antibodies and gave the highest values of absorbance. Positive ELISA values were obtained up to dilution of 1:204800 with antiserum of the second collection. On the other hand, the indirect ELISA used to detect aflatoxin G1 in grain showed that 2 wheat samples and 7 barley samples gave a positive reaction with produced antiserum which possessed absorbency values were double that of the normal serum.