GC-MS Analysis of Spondias mombin (Linn) Methanol Leaf Extract

S. Okereke, O. Ikpeazu, C. J. Nwaogwugwu, Ahamefula Sunday Ezekwe, C. Nosiri, P. O. Ukogu, Duru Michael Osita, Akara Emmanuel
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Abstract

The bioactive components of Spondias mombin leaf have been evaluated using gas chromatography and mass spectroscopy (GC/MS). The plant were collected washed, shade dried and milled and the methanol extract of the leaf plant were prepared by soxhlet extract method. The methanol extract were analyzed for the identification of phytochemical compounds presenting the Spondias mombin plant leaf using GC/MS methods. Result of the GC-MS analysis of Spondias mombin leaf extract leads to the identification of 8 compounds. The identification of phytochemicals was based on the Molecular Weight, molecular formula, RT (Retention Time) and peak area % (concentration). These peaks indicated the presence of eight bioactive compounds in the extract. The names, molecular formular, percentage composition and molecular masses of these Short Research Article Okereke et al.; IJBCRR, 30(1): 1-7, 2021; Article no.IJBCRR.61245 2 compounds are shown in Table 1. The 8 bioactive compounds are as follows; 9oxononanoic acid,3-isopropoxy, 1,1,15,5,5 hexamethyl-3trimethylsioxytrosiloxane (C12H34O4Si4) ,1,4methanocycloocta(d)pyridazine, 1α4α4aα10α)(C13H2ON2),Hexasiloxane 1,1,3,3,5,5,7,7,9,9,11,11dodeca methyl,1,4 methanocycloocta (d) pyridazine 1,4,4a,5,6,9,10,10aoctalydro 11,11-dimethyl(1α,4α4aα10α), Hexasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11 dodecamethyl, 3isopropoxy-1,1,1,5,5,5 hexamethyl-3-(trimethylsiloxy) trisiloxane C12H34O4Si4 and 1,1,1,3,5,5,5Heptamethyltrisiloxane(C7H22O2Si3). It is concluded that these bioactive component are known to exhibit medicinal value as well as pharmacological activities.
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海参甲醇叶提取物的GC-MS分析
采用气相色谱-质谱联用技术(GC/MS)对海棘叶的生物活性成分进行了鉴定。收集植物,洗涤,遮荫干燥,研磨,用索氏提取法制备叶片植物的甲醇提取物。采用气相色谱/质谱联用技术对甲醇提取物进行鉴定。通过GC-MS分析,鉴定出8种化合物。根据分子量、分子式、RT(保留时间)和峰面积%(浓度)对植物化学物质进行鉴定。这些峰表明提取物中存在八种生物活性化合物。这些短研究文章的名称、分子式、百分比组成和分子质量;生物工程学报,30(1):1-7,2021;文章no.IJBCRR。61245 2个化合物如表1所示。8种生物活性化合物如下:9 oxononanoic酸,3-isopropoxy, 1, 1, 15日,5、5 hexamethyl-3trimethylsioxytrosiloxane (C12H34O4Si4), 1、4 methanocycloocta (d)哒嗪,4 1α4αα10α)(C13H2ON2) Hexasiloxane 1, 1, 3, 3, 5, 5, 7, 7日,9日,9日,11日,11 dodeca甲基,1、4 methanocycloocta (d)哒嗪1,4,4,5,6,9、10、10 aoctalydro 11日11-dimethyl 4(4 1α,αα10α),Hexasiloxane, 1, 1, 3, 3, 5, 5, 7, 7日,9日,9日,11日,11 dodecamethyl 3 isopropoxy-1, 1, 1、5、5、5 hexamethyl-3 - (trimethylsiloxy) trisiloxane C12H34O4Si4和1,1,1,3,5,5、5 heptamethyltrisiloxane (C7H22O2Si3)。这些生物活性成分不仅具有药理活性,而且具有一定的药用价值。
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