Utilizing Protein Purification Techniques to Characterize Protein Structure and Function

N. Varma, A. Arbab
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Abstract

Protein characterization is crucial for understanding the role played by proteins in different cellular processes. In addition, protein characterization is vital in biopharmaceutical industry, wherein proteins are produced in different extraneous hosts such as bacteria, yeast and mammalian cells. Recently, wide range of protein purification techniques are made available that can be utilized to solve protein structure and function. In this short review we like to highlight some of recent developments in utilizing protein purification techniques to characterize structures and functions.
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利用蛋白质纯化技术表征蛋白质结构和功能
蛋白质表征对于理解蛋白质在不同细胞过程中所起的作用至关重要。此外,蛋白质表征在生物制药工业中至关重要,其中蛋白质是在不同的外部宿主(如细菌,酵母和哺乳动物细胞)中产生的。近年来,广泛的蛋白质纯化技术可以用来解决蛋白质的结构和功能。在这篇简短的综述中,我们将重点介绍利用蛋白质纯化技术表征结构和功能的一些最新进展。
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