Effects of Gamma Irradiation on Wheat Gluten

M. Doguchi
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引用次数: 4

Abstract

The effects of γ-irradiation on wheat gluten were studied by means of gel filtration on Sephadex G-100, starch gel electrophoresis and analysis of amino acid composition. Analyses of gluten at a moisture content of 2% revealed no significant change in amino acid composition except for cystine which was decreased by about 8% with irradiation at 10 Mrad. Changes in the chromatogram from gel filtration were interpreted in terms of random depolymerization resulting from irradiation. The results of starch gel electrophoresis suggested that irradiation levels greater than 3 Mrad resulted in characteristic changes in the molecular configuration of gliadin components.
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γ辐照对小麦面筋的影响
采用Sephadex G-100凝胶过滤、淀粉凝胶电泳和氨基酸组成分析等方法研究了γ辐照对小麦面筋的影响。在水分含量为2%时对面筋进行分析,结果显示除了胱氨酸外,其氨基酸组成没有显著变化,在10 Mrad的辐照下胱氨酸减少了约8%。凝胶过滤后的色谱变化被解释为辐照引起的随机解聚。淀粉凝胶电泳结果表明,大于3 Mrad的辐照水平导致麦胶蛋白组分的分子构型发生特征性变化。
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