Protocol of Femur Extraction from Bats in Fluid-Preserved Collections

N. S. V. Louzada, Carlos Rodrigues de Moraes Neto, M. Nogueira, L. Pessôa
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Abstract

The anatomy of appendicular bones has been demonstrated to be informative for taxonomic, paleontological, and functional studies of bats. Its use in such studies, however, is limited by the small number of skeletons available for this taxonomic group in scientific collections. Here we describe a protocol for the extraction of the femur from fluid-preserved bats. This protocol was successfully tested on a large sample, including 58 species in 43 genera and 9 families (a total of 183 specimens), and is minimally invasive, requiring only two incisions in the leg at disarticulation points (knee and coxofemoral joints). This method provides material for appendicular anatomical research with minimal damage to external morphology.
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液体保存标本中蝙蝠股骨的提取方法
阑尾骨的解剖结构已被证明为蝙蝠的分类学、古生物学和功能研究提供了信息。然而,它在此类研究中的使用受到科学收藏中可用于该分类组的骨骼数量的限制。在这里,我们描述了一种从液体保存的蝙蝠中提取股骨的方案。该方案成功地在大样本上进行了测试,包括9科43属58种(共183个标本),并且是微创的,只需要在腿部脱节点(膝关节和髋股关节)上开两个切口。该方法为阑尾解剖研究提供了材料,对外部形态的损害最小。
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