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Operation Bone Rescue—A Case Study of Remediating Flood Damage to Mammal Specimens 骨抢救手术——以修复洪水对哺乳动物标本的损害为例
Pub Date : 2021-01-01 DOI: 10.14351/0831-4985-35.1.21
Erin K. Kuprewicz, F. M. Muzio, Greyson Nackid
Water damage to natural history collections can result from both natural and human-caused environmental disasters. Floods can result in irreparable damage to scientific specimens, depending on the scale of the disaster, types of specimens affected, and availability of remediation resources. In April 2021, the mammal skeletal collection in the Biodiversity Research Collections (BRC) of the University of Connecticut (UConn) experienced a ceiling flood that affected 612 specimens. In this paper we detail all steps of our specimen rescue process and all materials and equipment we used to complete this remediation in an endeavor we termed “Operation Bone Rescue.” Because we were able to immediately respond to this emergency and implement a complete remediation plan, facilitated by funding from our university, we not only rescued all water-affected specimens, but also improved specimen storage and metadata. We highlight the holistic nature of this successful operation and the key roles played by personnel in the BRC, UConn Facilities Operations, Fire Department, and College of Liberal Arts and Sciences Dean's Office. A deep appreciation of the value of natural history collections is shared widely on our campus and resulted in the favorable outcomes of this complex, coordinated specimen rescue effort.
自然灾害和人为环境灾害都可能对自然历史藏品造成水害。洪水可能对科学标本造成无法弥补的损害,具体取决于灾害的规模、受影响标本的类型以及补救资源的可用性。2021年4月,康涅狄格大学(UConn)生物多样性研究馆藏(BRC)的哺乳动物骨骼藏品经历了一次天花板洪水,影响了612个标本。在本文中,我们详细介绍了标本修复过程的所有步骤,以及我们用于完成修复的所有材料和设备,我们称之为“骨骼修复操作”。由于我们能够立即对这一紧急情况作出反应,并在学校的资助下实施了完整的修复计划,我们不仅救出了所有受水影响的标本,而且改善了标本的储存和元数据。我们强调了这次成功行动的整体性,以及BRC、康涅狄格大学设施运营、消防部门和文理学院院长办公室人员发挥的关键作用。在我们的校园里,人们对自然历史藏品的价值有着深刻的认识,这使得这项复杂而协调的标本救援工作取得了良好的成果。
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引用次数: 0
Fungus and Feathers: Combatting a Mold Outbreak in an Ornithological Collection 真菌和羽毛:在鸟类收藏中对抗霉菌爆发
Pub Date : 2021-01-01 DOI: 10.14351/0831-4985-35.1.32
P. Trail, Ariel M. Woodward, J. French
The ornithological collection of the National Fish and Wildlife Forensic Laboratory in Ashland, Oregon includes over 6,800 bird skin and loose feather specimens. These are essential reference material for the morphological identification of avian evidence in wildlife crime investigations by the U.S. Fish and Wildlife Service. In the summer of 2020, these specimens were moved from several locations and installed in a new building dedicated to the laboratory's bird, mammal, and herpetological collections. Following installation in the new building, a severe outbreak of mold was discovered in many of the cabinets containing bird specimens. This paper reports on the likely cause of the mold outbreak and the actions taken to control it, preserve the specimens, and prevent future outbreaks.
俄勒冈州阿什兰的国家鱼类和野生动物法医实验室的鸟类学收藏品包括6800多件鸟类皮肤和松散的羽毛标本。这些是美国鱼类和野生动物管理局在野生动物犯罪调查中对鸟类证据进行形态学鉴定的重要参考材料。在2020年夏天,这些标本从几个地方转移到一个专门用于实验室鸟类、哺乳动物和爬行动物收藏的新建筑中。在新楼安装后,在许多装有鸟类标本的橱柜中发现了严重的霉菌爆发。本文报告了霉菌爆发的可能原因以及采取的控制措施,保存标本,防止未来爆发。
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引用次数: 0
The Need for Permit Management within Biodiversity Collection Management Systems to Digitally Track Legal Compliance Documentation and Increase Transparency About Origins and Uses 在生物多样性收集管理系统中进行许可管理的必要性,以数字方式跟踪法律合规文件,提高来源和使用的透明度
Pub Date : 2021-01-01 DOI: 10.14351/0831-4985-35.1.1
Breda M Zimkus, L. Ford, Paul J. Morris
A growing number of domestic and international legal issues are confronting biodiversity collections, which require immediate access to information documenting the legal aspects of specimen ownership and restrictions regarding use. The Nagoya Protocol, which entered into force in 2014, established a legal framework for access and benefit-sharing of genetic resources and has notable implications for collecting, researchers working with specimens, and biodiversity collections. Herein, we discuss how this international protocol mandates operating changes within US biodiversity collections. Given the new legal landscape, it is clear that digital solutions for tracking records at all stages of a specimen's life cycle are needed. We outline how the Harvard Museum of Comparative Zoology (MCZ) has made changes to its procedures and museum-wide database, MCZbase (an independent instance of the Arctos collections management system), linking legal compliance documentation to specimens and transactions (i.e., accessions, loans). We used permits, certificates, and agreements associated with MCZ specimens accessioned in 2018 as a means to assess a new module created to track compliance documentation, a controlled vocabulary categorizing these documents, and the automatic linkages established among documentation, specimens, and transactions. While the emphasis of this work was a single year test case, its successful implementation may be informative to policies and collection management systems at other institutions.
生物多样性收集面临越来越多的国内和国际法律问题,这需要立即获得记录标本所有权和使用限制的法律方面的信息。《名古屋议定书》于2014年生效,为遗传资源的获取和惠益分享建立了法律框架,并对收集、研究标本的研究人员和生物多样性收集产生了显著影响。在此,我们讨论了该国际议定书如何要求美国生物多样性收集的操作变化。考虑到新的法律环境,很明显,需要在标本生命周期的各个阶段跟踪记录的数字解决方案。我们概述了哈佛比较动物学博物馆(MCZ)如何改变其程序和博物馆范围内的数据库MCZbase (Arctos藏品管理系统的独立实例),将法律合规文件与标本和交易(即,加入,借阅)联系起来。我们使用与2018年加入的MCZ标本相关的许可证、证书和协议作为评估新模块的手段,该模块用于跟踪合规性文件、对这些文件进行分类的受控词汇表,以及在文件、标本和交易之间建立的自动联系。虽然这项工作的重点是一年的测试案例,但其成功实施可能对其他机构的政策和收集管理系统提供信息。
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引用次数: 3
An Inexpensive Sample Cataloging and Retrieval Tool for Frozen Tissue Collections Using Powerpoint 用于冷冻组织收集的廉价样本编目和检索工具
Pub Date : 2021-01-01 DOI: 10.14351/0831-4985-35.1.48
T. E. Lee, Madeline L. Walicek
We present a method for the cataloging and retrieval of frozen tissue samples stored in a −80°C freezer via image databasing of the samples using server software (PowerPoint) and digital photography. This method is an inexpensive, computer-guided, rapid retrieval technique for visually locating frozen tissue samples in collections. This approach increases retrieval efficiency and reduces sample integrity risk.
我们提出了一种通过使用服务器软件(PowerPoint)和数码摄影对样本进行图像数据库的方法,对储存在−80°C冷冻库中的冷冻组织样本进行编目和检索。这种方法是一种廉价的、计算机引导的、快速检索技术,用于视觉定位收集的冷冻组织样本。该方法提高了检索效率,降低了样本完整性风险。
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引用次数: 0
Testing of Inexpensive Nitrogen Chambers for Maintenance of Herbarium Specimens 维持植物标本馆标本的廉价氮气室的测试
Pub Date : 2021-01-01 DOI: 10.14351/0831-4985-35.01.06
M. K. Van Zant, D. Lightfoot
Inexpensive vacuum bags, designed to store fabrics and made from oxygen-barrier plastic, were repurposed as ambient temperature nitrogen chambers to neutralize pests on herbarium specimens. Combination t-test and ANCOVA results on data from oxygen-monitoring tablets indicated 0.1% anaerobia was maintained inside these bags for 39 days. Anaerobia below 0.3% oxygen maintained for up to 3 weeks deactivates insects, bacteria, and most molds. One or two small oxygen-absorber packets placed inside bags affected how quickly and reliably 0.1% anaerobia could be achieved and maintained. Three vs. six flushes with nitrogen had no statistically significant effect for achieving anaerobia.
设计用于储存织物的廉价真空袋由氧气屏障塑料制成,被改造成常温氮气室,以中和植物标本馆标本上的害虫。氧监测片数据联合t检验和ANCOVA结果显示,这些袋内保持0.1%的厌氧状态39天。氧含量低于0.3%的厌氧菌维持3周,使昆虫、细菌和大多数霉菌失活。一个或两个小的吸氧包放在袋子里影响如何快速和可靠地达到和维持0.1%的厌氧。3次与6次氮冲洗对实现厌氧无统计学显著影响。
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引用次数: 0
Evaluating the Ethanol Levels and Storage Containers for Efficient Preservation of Historical Collections at the Museo De La Salle, Bogotá, Colombia 哥伦比亚波哥大<e:1> La Salle博物馆对有效保存历史藏品的乙醇含量和储存容器的评估
Pub Date : 2021-01-01 DOI: 10.14351/0831-4985-35.1.52
C. Cruz-Rodríguez, Julieth Stella Cárdenas, H. Ramírez-Chaves
The Museo de La Salle–Bogotá (MLS) houses biological specimens of which 30% (17,001 specimens) are stored in fluid (ethanol 70%). The collections have historical significance for documenting Colombian biodiversity and contain the oldest zoological specimens in the country. For these reasons, the curation and management of the specimens require special attention and endless vigilance. In this study we assessed the decrease in ethanol level in jars of the MLS fluid collections over a 3-year period from 2013 to 2016 and evaluated the relation between container and lid type and ethanol loss. We did not observe a relevant decrease in the percentage of ethanol levels during 2013–2016. However, the type of lid used influenced the amount of ethanol that evaporated; we found that the pressure and twist lids were more effective in maintaining the ethanol levels in the containers, since they have a pressurized rotation system that allows a more secure closure in contrast to the pressure cap, which does not prevent the evaporation of ethanol. Based on our evaluation, we suggest the use of pressure and twist closure caps or caps with a continuous thread closure type along with a tamper-evident seal between the jar mouth and the container cap to ensure a tight and secure seal for minimizing the risk of fluid loss and ensuring the preservation of fluid-fixed specimens of this historical collection.
拉萨尔-波哥大博物馆 (MLS)收藏了生物标本,其中30%(17,001个标本)储存在液体中(乙醇70%)。这些藏品对记录哥伦比亚的生物多样性具有历史意义,并包含该国最古老的动物标本。由于这些原因,标本的保管和管理需要特别注意和保持警惕。在这项研究中,我们评估了2013年至2016年3年间MLS液体收集罐中乙醇含量的下降,并评估了容器和盖子类型与乙醇损失之间的关系。在2013-2016年期间,我们没有观察到乙醇含量百分比的相关下降。然而,使用的盖子类型会影响蒸发乙醇的量;我们发现,压力盖和扭转盖在保持容器中的乙醇水平方面更有效,因为它们有一个加压旋转系统,与压力盖相比,它允许更安全的关闭,而压力盖不能阻止乙醇的蒸发。根据我们的评估,我们建议使用压力和扭转瓶盖或连续螺纹瓶盖,并在瓶口和瓶盖之间进行防破坏密封,以确保密封紧密和安全,以最大限度地减少液体流失的风险,并确保保存这一历史收藏品的液体固定标本。
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引用次数: 0
Seventeen Years With Jars: a Conclusion and a Small Overview About Plans 17年的罐子:一个结论和一个关于计划的小概述
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.135
A. Friederichs
There were more than 348 natural history collections and 179 medical collections in Germany compared with 68 natural history collections in France (Charon 2014). More than half of these have parts of the collections stored in fluids, and thus they will all use jars and will all face similar problems (e.g., regularly needing new jars and effectivemethods for sealing them). After 17 years of working with jars, moving wet collections, realizing the KUR-Projekt (Neuhaus et al. 2012), and conducting many workshops, I would like to give a short overview about future ideas and plans and a brief conclusion of my point of view. In most of the wet collections I have visited or worked with, such as the ones of the Museum für Naturkunde (MfN), the majority of the jars used are glass stopper jars (e.g., the Spider wet collection at theMfN shown in Fig. 1). However, such collections also contain a wide variety of other types of glass and plastic containers with a range of closures, including glass plates, plastic snap or screw-on lids, and jars sealed with Picein, an asphaltum– rubber compound (Simmons 2014). Some closures and containers are efficient and durable for the long-term preservation of specimens, and in the past 10 years, both soda glass with twist-off lids and borosilicate glass jars have been used. Yet the question always arises related to the management of these collections: which are the best? Is it preferable to actually use borosilicate glass jars, reputed to be effective but expensive, or is it enough to consider soda–lime twist-off glass jars, which are less expensive? To answer the question, it does not seem sufficient to consider only the price and availability of these containers. Other factors are important, including the need to ensure a proper seal, consideration of the jar contents, and the need for institutions to share resources. For more than 100 years, collection assistants, curators, and collectionmanagers have been dealing with jars and have developed a large pool of knowledge and experience. While there are existing networks to share this knowledge, such as the Society for the Preservation of Natural History Collections (SPNHC) wiki (https://spnhc.biowikifarm.net/wiki) or the Natural Sciences Collections Association (NatSCA) and Global Conservation Forum lists, they are not exclusively dedicated to issues related to fluid collections. The day-to-day management of a fluid collection requires special expertise and attention to detail. The people in charge of the conservation of these collections have their own knowledge and experience to draw on, but it is difficult to find this information in books or articles. Most of the time, this knowledge is exchanged with colleagues in the museum or sometimes with well-known colleagues in the field working in other museums around the world. Beyond this small group, it is difficult at the moment to share one’s own knowledge or to write articles detailing these facts. It is only in recent ye
德国有超过348个博物馆藏和179个医学馆藏,而法国有68个博物馆藏(Charon 2014)。其中一半以上将部分收集物储存在液体中,因此它们都将使用罐子,并且都将面临类似的问题(例如,定期需要新的罐子和有效的密封方法)。经过17年的罐子工作,移动湿收藏品,实现KUR-Projekt (Neuhaus et al. 2012),并举办了许多研讨会,我想简要概述一下未来的想法和计划,并简要总结一下我的观点。在大多数湿收集我去过或共事,比如博物馆的皮毛Naturkunde (MfN),大多数使用的罐子是玻璃塞罐(如蜘蛛湿收集theMfN图1所示)。然而,这样的集合也包含各种各样的其他类型的玻璃和塑料容器的闭包,包括玻璃板、塑料吸附或用螺钉固定的盖子,并与Picein罐子密封,沥青-橡胶复合西蒙斯(2014)。一些瓶盖和容器对于标本的长期保存是有效和耐用的,在过去的10年里,有拧开盖子的苏打玻璃和硼硅玻璃罐都被使用了。然而,与这些集合的管理相关的问题总是出现:哪些是最好的?是使用硼硅玻璃罐更可取,它被认为是有效的,但昂贵的,还是考虑钠石灰扭转玻璃罐,它更便宜?要回答这个问题,仅仅考虑这些容器的价格和可用性似乎是不够的。其他因素也很重要,包括需要确保适当的密封,考虑罐子的内容,以及机构共享资源的需要。一百多年来,收藏助理、馆长和收藏经理一直在处理罐子,并积累了大量的知识和经验。虽然有现有的网络来分享这些知识,如自然历史藏品保护协会(SPNHC) wiki (https://spnhc.biowikifarm.net/wiki)或自然科学藏品协会(NatSCA)和全球保护论坛列表,但它们并不专门致力于与流体收藏相关的问题。液体收集的日常管理需要特殊的专业知识和对细节的关注。负责保护这些藏品的人有自己的知识和经验可以借鉴,但很难在书籍或文章中找到这些信息。大多数时候,这些知识是与博物馆的同事交流的,有时是与在世界其他博物馆工作的该领域的知名同事交流。除了这一小群人之外,目前很难分享自己的知识或撰写详细描述这些事实的文章。直到最近几年,我们才开始理解这些技术知识代表了什么,以及如果不维护这些知识库,我们可能会失去什么。管理流体收集的挑战凸显了以下需求
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引用次数: 0
Benefits for the Management and Preservation of Fluid Collections Using QR Codes 使用QR码管理和保存液体收集的好处
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.170
P. Pruvost
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引用次数: 0
Noninvasive Fluid Identification: Potential of Micro-Raman Spectroscopy 无创流体鉴定:微拉曼光谱的潜力
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.53
S. Cersoy, V. Rouchon, O. Belhadj, J. Cuisin, M. Herbin
Conservation of the preserving medium is an essential element for the proper preservation of specimens in fluid collections. However, the preservatives can become chemically altered over time or be lost by processes such as evaporation. To combat such changes and properly care for and maintain immersed specimens, it is therefore necessary to know what preservative fluid was initially chosen and how its chemistry may have evolved with time. The present work explores the possibilities offered by Raman spectrometry for a rapid, nondestructive, noninvasive alternative to commonly employed chemical identification tests, which are often limited to the identification of simple fluids. In a first step, fluids were reconstituted and analyzed in small standard glass containers to evaluate the potential of the technique. Then we successfully applied the procedure to real cases and considered its possible use to estimate the concentration of ethanol and to detect small quantities of formaldehyde (down to 1%). The results demonstrate the power of this technique, which opens up new possibilities for the management of fluid collections.
保存介质的保存是液体标本妥善保存的基本要素。然而,防腐剂会随着时间的推移而发生化学变化或因蒸发等过程而丢失。因此,为了对抗这种变化,并正确地照顾和维护浸泡在水中的标本,有必要知道最初选择的防腐剂是什么,以及它的化学成分如何随着时间的推移而演变。本研究探讨了拉曼光谱法作为一种快速、非破坏性、非侵入性的化学鉴定测试替代方法的可能性,这种方法通常仅限于简单流体的鉴定。第一步,在小型标准玻璃容器中对流体进行重构和分析,以评估该技术的潜力。然后,我们成功地将该程序应用于实际情况,并考虑其可能用于估计乙醇浓度和检测少量甲醛(低至1%)。结果证明了该技术的强大功能,为流体收集管理开辟了新的可能性。
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引用次数: 2
The Museum of Anatomy and Embryology Louis Deroubaix from the Université Libre de Bruxelles (ULB): Wet and Other Collections 来自布鲁塞尔自由大学(ULB)的解剖学和胚胎学博物馆:湿和其他藏品
Pub Date : 2020-01-01 DOI: 10.14351/0831-4985-34.1.114
S. Louryan, N. Vanmuylder, M. Daumas
The Museum of Anatomy and Embryology of the Université Libre de Bruxelles (ULB), part of the Laboratory of Anatomy, Biomechanics, and Organogenesis (LABO), was founded around 1838 by the anatomist and surgeon Louis Deroubaix (1813–1897) shortly after the ULB was established (Kinnaert and Louryan 2009, Louryan et al. 2010). Initially, the Museum of Anatomy and Embryology contained human specimens from the main old hospitals of Brussels. Further anatomical parts were gradually collected and dissected by successive teachers of human anatomy and their team thanks to a legal system of body donation. Since the museum’s inception, many bone and soft tissue dissections, preserved in liquid medium, have contributed to the enrichment of the collections for pedagogical purposes. Following the faculty of medicine, the museum’s geographical location has changed four times across Brussels, from the Charles de Lorraine’s palace in the city center to the present location on the periphery of the city in the university’s medical campus. An extensive veterinary bone collection is available for students in a separate building. Resulting from faculty turnover and successive curators’ areas of interest, this museum diversified over time. Figure 1 displays a general overview of the museum, including fluid collections. Various plaster orwaxmodels (e.g.,His-Stegermodels, Tramond’s wax castings, and castings from the famous collection created by Seifert and Nicolas) were progressively added, as was teratological material. As an example, Figure 2 shows a three-dimensional CT scan reformation of a newborn (preserved in fluid) with severe aprosopia (complex and rare facial malformation) as well as pathological, anthropological, odontological, and comparative anatomy specimens. New dissections, many of which are plastinated, are regularly added to the collections, while most past acquisitions were made by incorporating other collections or private donations. Plastinated material enhances material conservation and makes transport in the classrooms easier. The museum currently includes about 1,500 specimens, including fluid-preserved and dry material plus the osteological teaching collection. The Museum of Anatomy and Embryology was founded for pedagogical purposes. Therefore, most of the specimens (fluid-preserved or other) are displayed using a camera during anatomy and embryology lessons. Anatomy and embryology courses take part in a room adjacent to the museum. Moreover, specimens are displayed during anatomical demonstrations and practical sessions, alternatively using medical imaging documents and a virtual anatomy dissection table. During the learning process, switching from a booksize paper illustration to a three-dimensional image is challenging. Using real anatomical specimens helps students and allows them, among other things, to observe the scale and texture of structures to develop deeper and more extensive knowledge. Because usage of specimens can lead to damage,
布鲁塞尔自由大学(ULB)解剖学和胚胎学博物馆是解剖学,生物力学和器官发生实验室(LABO)的一部分,在ULB成立后不久,解剖学家和外科医生Louis Deroubaix(1813-1897)于1838年左右成立(Kinnaert and Louryan 2009, Louryan et al. 2010)。最初,解剖学和胚胎学博物馆收藏了来自布鲁塞尔主要老医院的人体标本。由于遗体捐赠的法律制度,后来的人体解剖学老师和他们的团队逐渐收集和解剖了更多的解剖部位。自博物馆成立以来,许多骨骼和软组织解剖,保存在液体介质中,有助于丰富教学目的的收藏。继医学院之后,博物馆的地理位置在布鲁塞尔发生了四次变化,从市中心的查尔斯·德·洛林宫殿到现在位于城市外围的大学医学院校园。在一个单独的建筑里,有一个广泛的兽医骨骼收藏供学生使用。由于教师更替和连续策展人感兴趣的领域,这个博物馆随着时间的推移而多样化。图1显示了博物馆的总体概况,包括流体收藏。各种石膏或蜡模型(例如,他的斯蒂格模型,特拉蒙的蜡铸件,以及由塞弗特和尼古拉斯创作的著名系列的铸件)逐渐增加,畸形材料也逐渐增加。例如,图2显示了一个新生儿(保存在液体中)严重斜视(复杂而罕见的面部畸形)的三维CT扫描改造,以及病理、人类学、牙科学和比较解剖学标本。新的解剖,其中许多是塑化的,定期添加到收藏中,而过去的大多数收购是通过合并其他收藏或私人捐赠获得的。塑化材料提高了材料的节约,使教室内的交通更加方便。该博物馆目前收藏了大约1500件标本,包括液体保存的和干燥的材料,以及骨学教学收藏。解剖学和胚胎学博物馆是为了教学目的而建立的。因此,在解剖学和胚胎学课程中,大多数标本(液体保存或其他)都是用相机展示的。解剖学和胚胎学课程在博物馆旁边的一个房间里进行。此外,标本在解剖演示和实践会议中展示,或者使用医学成像文件和虚拟解剖解剖台。在学习过程中,从书本大小的纸质插图切换到三维图像是具有挑战性的。使用真实的解剖标本可以帮助学生,并允许他们,除其他事项外,观察结构的规模和纹理,以发展更深更广泛的知识。由于标本的使用可能导致损坏,每次从瓶中取出标本后,必须仔细监测标本的状况。维护标本需要定期更换
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引用次数: 0
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Collection Forum
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