Effect of the Combination of Native Herb with Motherwort on Anti-Inflammatory, Anti-Fatigue, and Hypertensive Hypoxia Tolerance

Abstract

Objective: To investigate the in vivo anti-inflammatory, anti-fatigue, and hypertensive hypoxia tolerance effects of earth ginseng with motherwort and its combination on mice. Methods: Male mice were divided into 6 groups according to their body weight: blank control group, native ginseng group, motherwort group, and three experimental groups with a 1:1 ratio of native ginseng and motherwort. 1.5, 4.5, and 7.5 g-kg-1 -d-1 were given to the three experimental groups. The blank control group was instilled with saline, and the earth ginseng group and motherwort group were given 1.5 g-kg -d-1-1 of the corresponding extracts. After 21 d of continuous gavage, the ear swelling test by xylene, the weight swimming test, and the high-pressure hypoxia tolerance test were performed. Also, the experimental indexes were measured, the liver weight was weighed, and the liver weight coefficient was calculated. Results: In the anti-inflammatory experiment, there was a significant difference in the swelling inhibition effect between the two single-flavored groups compared with the control group, the low-dose group compared with the two single-flavored groups, and the medium- and high-dose groups compared with the low-dose group (P < 0.05). In the weight swimming experiment and high-pressure hypoxia tolerance experiment, compared with the control group, the mice in the earth ginseng group and the motherwort group showed a significant improvement in weight swimming time and high-pressure hypoxia tolerance time (P＜0.05); compared with the two single herb groups, the mice in the low dose group showed a statistically significant improvement in weight swimming time and high-pressure hypoxia tolerance time (P＜0.05); compared with the low dose group, the mice in the high dose group showed a statistically significant improvement in weight swimming time and high-pressure hypoxia tolerance time (P＜0.05). Compared with the low-dose group, the high-dose group had a statistically significant (P < 0.05) improvement in weight-bearing swimming time and hyperbaric hypoxia tolerance time. Compared with the control group, the liver weight coefficient of the earth ginseng group was statistically significant (P＜0.05); compared with the two single herb groups, the liver weight coefficient of the low-dose group was statistically significant (P＜0.05); compared with the low-dose group, the liver weight coefficient of the middle and high-dose groups were statistically significant (P＜0.05). Conclusion: Native herbal medicine and its combination have significant anti-inflammatory, anti-fatigue, and high-pressure hypoxia tolerance effects, and the anti-inflammatory effect was positively correlated with the increase of the extract concentration; the anti-fatigue and high-pressure hypoxia tolerance effects were the best in the high-dose group.