Reverse Phase HPLC Profiling of Antioxidant and Antimicrobial Activities of Sarcochlamys pulcherrima (Roxb.) Gaud.

Pradip Doley, N. M. Devi, C. Singh, Abhishekh Singh
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Abstract

The total phenolics content, total flavonoids content, antioxidant activity and antimicrobial activity of methanol extract and its derived fractions from Sarcochlamys pulcherrima (Roxb.) Gaud. were evaluated. The plant material was initially extracted with methanol. The fractionation was carried out using liquid-liquid extraction with hexane, ethyl acetate, n-butanol, and water. The Folin-Ciocalteu assay was used to quantify total phenolics content, Aluminum Chloride assay to quantify total flavonoids content, DPPH (2, 2-diphenyl-1-picrylhydrazine) and reducing power assay are to determine antioxidant activity. The ethyl acetate fraction was observed to contain high phenolic content (201.37 mg/g) followed by n-butanol fraction (197.76 mg/g), methanol extract (136.93mg), water fraction (55.05mg/g) and hexane fraction (36.94mg/g) respectively. The flavonoids content ranges from 109.48 mg/g to 8.63 mg/g dry extract. The highest antioxidant activity was found in n-butanol fraction (EC 50 =14.69 µg/mL in DPPH and 162.65 µg/mL in reducing power). The antimicrobial activity of the samples was evaluated based on inhibition zone using agar-well diffusion assay and minimal inhibition concentration (MIC) values. Hexane fraction had no antimicrobial effect against test microorganisms. The zone of inhibitions ranged from 11 mm to 6 mm. The n-butanol fraction possessed the highest antimicrobial activity against E. coli among the four fractions with MIC value of 0.781mg/mL. In addition rutin, gallic acid, quercetin and kaempferol were quantified by reverse phase high performance liquid chromatography (HPLC) method. In ethyl acetate and n-butanol fraction, observed large amount rutin (77.53 ± 7.57 mg/g; 77.45 ± 1.71 mg/g) and gallic acid (61.01 ± 2.34 mg/g; 22.75 ± 1.54 mg/g) as compared to the quercetin and kaempferol. Doley et al., International Current Pharmaceutical Journal, May 2016, 5(6): 49-54
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白斑麻(Sarcochlamys pulcherrima, Roxb.)抗氧化和抗菌活性的反相HPLC分析俗气东西。
研究了石竹草甲醇提取物及其衍生组分的总酚类物质含量、总黄酮含量、抗氧化活性和抗菌活性。俗气东西。进行了评估。植物材料最初用甲醇提取。采用正己烷、乙酸乙酯、正丁醇和水的液液萃取法进行分馏。用Folin-Ciocalteu法测定总酚含量,用氯化铝法测定总黄酮含量,用DPPH(2,2 -二苯基-1-苦味肼)和还原力法测定抗氧化活性。乙酸乙酯部位酚类物质含量最高(201.37 mg/g),其次是正丁醇部位(197.76 mg/g)、甲醇部位(136.93mg)、水部位(55.05mg/g)和己烷部位(36.94mg/g)。干提取物总黄酮含量为109.48 ~ 8.63 mg/g。其中,正丁醇部分的抗氧化活性最高(ec50 =14.69µg/mL,还原力为162.65µg/mL)。采用琼脂孔扩散法和最小抑菌浓度(MIC)对样品的抑菌活性进行评价。己烷馏分对试验微生物无抑菌作用。抑制区范围为11 ~ 6 mm。正丁醇部分对大肠杆菌的抑菌活性最高,MIC值为0.781mg/mL。采用反相高效液相色谱法对芦丁、没食子酸、槲皮素和山奈酚进行定量分析。在乙酸乙酯和正丁醇馏分中,观察到大量芦丁(77.53±7.57 mg/g);77.45±1.71 mg/g)和没食子酸(61.01±2.34 mg/g;22.75±1.54 mg/g),与槲皮素和山奈酚相比。Doley等,国际现代医药杂志,2016,5(6):49-54
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