Physiological and genotoxic responses of the earthworm Aporrectodea caliginosa exposed to sublethal concentrations of AgNPs

IF 1.1 Q3 BIOLOGY Journal of Basic and Applied Zoology Pub Date : 2016-03-01 DOI:10.1016/j.jobaz.2015.12.004

Abdelmonem M. Khalil

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引用次数: 14

Abstract

The purpose of the current study was to measure the ecotoxicity of silver nanoparticles (AgNPs) on Aporrectodea caliginosa earthworm. No adult earthworm mortality was observed at any treatment during the sub-chronic exposure period (28 d). Biomass and cellulase levels reduced in a concentration-dependent manner in the exposed earthworms compared to those of the control. The hatched cocoons from the contaminated substrates were significantly (p < 0.05) fewer than that of the control substrate. This finding provided further support for the conclusion that AgNPs may affect cocoon hatchability. Cocoon hatchability could therefore be a more reliable endpoint at a specific concentration than cocoon production. A significant increase in DNA damage was revealed in the earthworms treated with AgNPs compared to the untreated ones. The results denoted the effectiveness of measuring cellulase activity, biomass, reproduction and DNA damage and reinforced the application of the present methods in nanoparticles pollution biomonitoring studies.

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暴露于亚致死浓度AgNPs的蚯蚓的生理和基因毒性反应

本研究旨在测定银纳米颗粒(AgNPs)对caliginosa Aporrectodea蚯蚓的生态毒性。在亚慢性暴露期间(28 d)，在任何处理下均未观察到成虫死亡。与对照组相比，暴露蚯蚓的生物量和纤维素酶水平呈浓度依赖性下降。从污染基质中孵化出的茧显著(p <0.05)低于对照底物。这一发现进一步支持了AgNPs可能影响茧孵化率的结论。因此，在特定浓度下，茧孵化率可能是比茧产量更可靠的终点。与未处理的蚯蚓相比，用AgNPs处理的蚯蚓的DNA损伤显著增加。结果表明，该方法在测定纤维素酶活性、生物量、繁殖和DNA损伤方面是有效的，并为该方法在纳米颗粒污染生物监测研究中的应用提供了依据。

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Journal of Basic and Applied Zoology

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47 weeks