Monitoring of Microtubers Virus Tested-Derived Potato Tissue Culture by DNA Fingerprint Analysis

Abstract

A simple ISSR-PCR as a routine method of microtuber PVY tested derived potato plantlets for somaclonal variations is aperquist for pricise monitoring of quality control during rapid mass micropropagation. and effective management of microtubers genetic resoures. This study reports on the use of ISSR-PCR for detection of genetic variations in micripropagated potato plants. Microtubers PVY testedderived potato plantlets were screened using ISSR-DNA markers. Three ISSR primers were chosen as producing polymorphic D N A fragments differentiating the investigated plantlets and microtubers in vitro. D N A fingerprint revealed genetic variations, 4 0 % polymorphisms of therapeutic plantlets, approximately 5 0 % of the analyzed potato plantlets with 4.5 polymorphic fragments per primer. While the D N A was isolated from microtubers produced using jasmonic acid and coumarin after ISSR amplification it was obvious that microtuber identical fragments profile. The frequency of somaclonal variations was found to be virus therapeutic and microtuberization inducers. The somaclonal variations were only detected in high jasmonic and coumarin concentrations. Although minor morphological variations were recorded in the microtubers of some clones. The developed fragments profiles of different micropropagated clones were typical to that of the donor mother plants.