Aplikasi Antibakteri Nanopartikel Perak (NPAg) Hasil Biosintesis dengan Ekstrak Air Daun Kemangi

G. Lestari, Pande Made Desy Ratnasari, J. Sibarani
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Abstract

Nanotechnology is a technology that can be used to overcome several environmental problems. Synthesis of silver nanoparticles (AgNPs) mediated with basil leaf bioreductant with a concentration 0.5% at a temperature of 25⁰C has been carried out.  The result of biosynthesis of AgNPs was analyzed using a UV-Vis spectrophotometer in which the SPR band showed the maximum wavelength of 429 nm. The size of AgNPs was determined by a Particle Size Analyzer (PSA), which its size was 86.83 nm. The morphology and elemental content of AgNPs were confirmed using SEM-EDS showing that the shape of AgNPs was irregularly spherical crystals while the EDS results showed a dominant peak at 3 keV indicating silver content. AgNPs showed strong antibacterial activity against Escherechia coli and moderate against Staphylococcus aureus.
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用罗勒水提取物合成的生物纳米粒子应用
纳米技术是一种可以用来克服几个环境问题的技术。在25⁰C的温度下,用浓度为0.5%的罗勒叶生物还原剂介导的银纳米颗粒(AgNPs)的合成已经进行。用紫外-可见分光光度计分析AgNPs的生物合成结果,SPR波段的最大波长为429 nm。通过粒径分析仪(PSA)测定AgNPs的粒径为86.83 nm。利用SEM-EDS对AgNPs的形貌和元素含量进行了分析,结果表明AgNPs的形状为不规则的球形晶体,EDS结果显示在3 keV处的优势峰表明银的含量。AgNPs对大肠杆菌有较强的抑菌活性,对金黄色葡萄球菌有中等的抑菌活性。
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