Evaluation of hepatoprotective potential of ethanolic extract of Ixora pavetta against isoniazid and rifampicin induced hepatotoxicity in rats

Abstract

Objective

The aim of present study was to demonstrate the hepatoprotective effect of ethanolic extract of Ixora pavetta (EEIP) against isoniazid and rifampicin induced hepatotoxicity in rats.

Method

Rats were divided into five groups each group containing 6 animals. All rats were treated with INH + RIF in saline water at the dose of 100 mg/kg b.w., p.o. to the experimental animals for 21 days. Group I served as control administered with distilled water. In order to study the effect of EEIP in rats, 200 mg/kg b.w. and 400 mg/kg b.w. of extracts were administered to the rats in group IV and V by oral route. Silymarin (2.5 mg/kg b.w., p.o.) was used as a standard drug in this study. After the course of treatment the animals were sacrificed and blood and liver samples were collected for biochemical and histopathological studies respectively.

Results

Biochemical parametric evaluation of both the standard and EEIP (200 mg and 400 mg/kg b. wt) treated rats showed significant decrease in SGOT, SGPT, ALP, Total Bilirubin, Direct Bilirubin, and Total cholesterol. And the level of Total protein was significantly increased in both standard and EEIP treated rats when compared to toxic control group rats. The changes in biochemical parameters were supported by histological profile.

Conclusion

It is concluded that the ethanolic extract of Ixora pavetta protects against rifampicin and Isoniazid-induced oxidative liver injury in rats.