Effect of turmeric extract and dimethyl sulfoxide on buffalo semen freezability and fertility

Abstract

The freeze-thaw process results in structural and functional damages caused by overaccumulation of reactive oxygen species (ROS). Addition of antioxidants to semen extenders is of a great importance to overcome this oxidative damage. The study objective was to evaluate the consequence of using Tris-citric acid fructose egg yolk (TCFY) extender supplemented with a combination of turmeric extract and dimethyl sulfoxide (DMSO) [TTD] on sperm freezability and fertility. From five tubes (each containing 5 mL TCFY), the first tube contained neither turmeric extract nor DMSO and was kept as control. The other four tubes contained 1.5% DMSO plus 100 µL/5 mL, 200 µL/5 mL, 300 µL/5 mL and 400 µL/5 mL turmeric extract. Semen samples were pooled and extended to reach an application of 60×106 sperm/mL (TT1D to TT4D, respectively). Diluted semen was exposed to the freezing protocol. The post cooling results revealed significant improvement in percent of alive spermatozoa in TT1D, significant decrease in sperm abnormalities in all concentrations used, significant improvement in intact acrosome percentage in TT1D, TT2D and TT4D. The post thawing results exhibited significant improvement in sperm motility in TT1D, TT2D and TT4D; significant amelioration of sperm membrane integrity (HOST) in TT1D, TT2D, TT3D and TT4D. Acrosome integrity was maintained in all concentrations as in the control. It was concluded that TT1D revealed the best semen quality in cooled semen; and TT1D, TT2D exhibited the superior post thawing semen quality. In addition, conception rate (CR) of the post-thawed semen was ameliorated in TT1D, TT2D, TT3D and TT4D with TT1D being the best one with this regard.