ABOUT POSSIBILITY OF APPLYING DERIVATIVE SPECTROPHOTOMETRY FOR BILIRUBIN ASSAY IN HUMAN ALBUMIN MEDICINAL PREPARATIONS

Abstract

When obtaining human albumin medicinal preparations with a high binding capacity to be used in detoxification therapy, an urgent task is to control residual content of bilirubin. Hence the purpose of this investigation is to study the possibility of applying derivative spectrophotometry for bilirubin assay in human albumin medicinal preparations. Record of the first-order spectra in combination with the spike test made it possible to obtain a linear dependence of the value |dA/dλ| in the minimum of the absorption band at a wavelength of 516 nm from the concentration of added bilirubin and to determine statistically significant bilirubin concentration in the medicinal preparation "Albupharm, solution for infusions, 200mg/ml" (JV Pharmland LLC, Republic of Belarus), which was 8,58 ± 0,23 μM, and also to draw a conclusion about the efficiency of purification technology of albumin from bilirubin.