Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.38
O. I. Lazovskaya, V. Senchuk, V. Leontiev
When obtaining human albumin medicinal preparations with a high binding capacity to be used in detoxification therapy, an urgent task is to control residual content of bilirubin. Hence the purpose of this investigation is to study the possibility of applying derivative spectrophotometry for bilirubin assay in human albumin medicinal preparations. Record of the first-order spectra in combination with the spike test made it possible to obtain a linear dependence of the value |dA/dλ| in the minimum of the absorption band at a wavelength of 516 nm from the concentration of added bilirubin and to determine statistically significant bilirubin concentration in the medicinal preparation "Albupharm, solution for infusions, 200mg/ml" (JV Pharmland LLC, Republic of Belarus), which was 8,58 ± 0,23 μM, and also to draw a conclusion about the efficiency of purification technology of albumin from bilirubin.
{"title":"ABOUT POSSIBILITY OF APPLYING DERIVATIVE SPECTROPHOTOMETRY FOR BILIRUBIN ASSAY IN HUMAN ALBUMIN MEDICINAL PREPARATIONS","authors":"O. I. Lazovskaya, V. Senchuk, V. Leontiev","doi":"10.52540/2074-9457.2021.4.38","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.38","url":null,"abstract":"When obtaining human albumin medicinal preparations with a high binding capacity to be used in detoxification therapy, an urgent task is to control residual content of bilirubin. Hence the purpose of this investigation is to study the possibility of applying derivative spectrophotometry for bilirubin assay in human albumin medicinal preparations. Record of the first-order spectra in combination with the spike test made it possible to obtain a linear dependence of the value |dA/dλ| in the minimum of the absorption band at a wavelength of 516 nm from the concentration of added bilirubin and to determine statistically significant bilirubin concentration in the medicinal preparation \"Albupharm, solution for infusions, 200mg/ml\" (JV Pharmland LLC, Republic of Belarus), which was 8,58 ± 0,23 μM, and also to draw a conclusion about the efficiency of purification technology of albumin from bilirubin.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85302991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.74
G. Buzuk
The aim of this work was to develop a way to adjust the effect of soil humidity on the determination of specific electrical resistance (SER). This goal is achieved by the fact that measuring is made using a capacitive sensor of volumetric soil humidity simultaneously with the measurement of SER using a 4-electrode F.F. Wenner array. Based on the analysis of literary and own data equations for adjusting SER against the background of different volumetric humidity and soil porosity are proposed. Further refinement of SER measurement results can be achieved by including in the equation the parameters related to the cation-exchange capacity (CAC) of the soil, namely the content of organic matter and clay particles. It is suggested that it is possible to determine expressly the latter using color-gauge (RGB) sensors.
{"title":"DETERMINATION OF SOIL TROPHIСITY BY ELECTROPHYSICAL METHOD. MESSAGE 3. ADJUSTMENT OF HUMIDITY INFLUENCE","authors":"G. Buzuk","doi":"10.52540/2074-9457.2021.4.74","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.74","url":null,"abstract":"The aim of this work was to develop a way to adjust the effect of soil humidity on the determination of specific electrical resistance (SER). This goal is achieved by the fact that measuring is made using a capacitive sensor of volumetric soil humidity simultaneously with the measurement of SER using a 4-electrode F.F. Wenner array. Based on the analysis of literary and own data equations for adjusting SER against the background of different volumetric humidity and soil porosity are proposed. Further refinement of SER measurement results can be achieved by including in the equation the parameters related to the cation-exchange capacity (CAC) of the soil, namely the content of organic matter and clay particles. It is suggested that it is possible to determine expressly the latter using color-gauge (RGB) sensors.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89131868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.46
G. Buzuk
The purpose of this work is to determine the influence of the electrodes surface isolation, as well as the method of geometric coefficient calculation, on the results of determining specific electrical resistance of soils (ER or SER). Metrological characteristics for SER measurement, as well as the influence of the electrodes immersion depth into the substrate studied on this indicator, were determined. Nonlinear nature of SER dependence on the electrodes immersion depth was established. For non-insulated electrodes the dependence was well approximated by the power function regardless of the method of calculating geometric coefficient of installation. In case of partially isolated (point) electrodes the use of geometric coefficient for calculating geometric coefficient with an adjustment to the electrodes immersion depth into the substrate studied makes it possible practically to get rid of SER dependence on the electrodes immersion depth. The technique can be used to assess the soils trophicity in places where medicinal plants grow.
{"title":"DETERMINATION OF SOIL TROPHICITY WITH ELECTROPHYSICAL METHOD. MESSAGE 2. ELECTRODE DESIGN AND THE METHOD OF GEOMETRIC COEFFICIENT CALCULATION","authors":"G. Buzuk","doi":"10.52540/2074-9457.2021.4.46","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.46","url":null,"abstract":"The purpose of this work is to determine the influence of the electrodes surface isolation, as well as the method of geometric coefficient calculation, on the results of determining specific electrical resistance of soils (ER or SER). Metrological characteristics for SER measurement, as well as the influence of the electrodes immersion depth into the substrate studied on this indicator, were determined. Nonlinear nature of SER dependence on the electrodes immersion depth was established. For non-insulated electrodes the dependence was well approximated by the power function regardless of the method of calculating geometric coefficient of installation. In case of partially isolated (point) electrodes the use of geometric coefficient for calculating geometric coefficient with an adjustment to the electrodes immersion depth into the substrate studied makes it possible practically to get rid of SER dependence on the electrodes immersion depth. The technique can be used to assess the soils trophicity in places where medicinal plants grow.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77699811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.9
D. Levushkin, I. V. Gravel
Medicinal plant raw material contains not only biologically active substances but also mineral elements. Currently, medicinal herbal preparations as sources of macronutrients are practically not studied. The aim of the research is to study the amount of macronutrients that can promote to manifestation of therapeutic effects on the example of a pectoral species № 4. An analysis of macronutrients content (potassium, sodium, magnesium, calcium, phosphorus, sulfur and iron) and its components in a pectoral species №4 was carried out using the atomic emission spectrometry. It was found out that the content of macronutrients is in the range of 62,1-21705,63 mg/kg. Their average values decreased in the series: K> Ca > Mg > P > S > Na > Fe. Macronutrients appear in the amount of 10 to 80% in the infusion from the pectoral species № 4. The amount of potassium, sodium, magnesium, calcium, phosphorus, sulfur and iron in the pectoral species № 4 made 25-1100% of the daily requirement which allows to consider this species as an additional source of those macronutrients presence in the human body.
药用植物原料中不仅含有生物活性物质，还含有矿质元素。目前，草药制剂作为宏量营养素来源的研究几乎没有。该研究的目的是研究大量营养素的量，可以促进治疗效果的表现，对胸肌物种的例子№4。使用原子发射光谱法分析了胸肌物种№4中的常量营养素含量(钾，钠，镁，钙，磷，硫和铁)及其组分。测定其常量营养素含量为62、1 ~ 21705、63 mg/kg。其平均值依次递减:K> Ca > Mg > P > S > Na > Fe。宏量营养素出现在10至80%的量输注从胸肌物种№4。钾、钠、镁、钙、磷、硫和铁在胸肌物种№4中的含量达到每日需求量的25-1100%，这使得可以将该物种视为人体中存在的这些宏量营养素的额外来源。
{"title":"MULTICOMPONENT MEDICINAL SPECIES AS A SOURCE OF MACRONUTRIENTS","authors":"D. Levushkin, I. V. Gravel","doi":"10.52540/2074-9457.2021.4.9","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.9","url":null,"abstract":"Medicinal plant raw material contains not only biologically active substances but also mineral elements. Currently, medicinal herbal preparations as sources of macronutrients are practically not studied. The aim of the research is to study the amount of macronutrients that can promote to manifestation of therapeutic effects on the example of a pectoral species № 4. An analysis of macronutrients content (potassium, sodium, magnesium, calcium, phosphorus, sulfur and iron) and its components in a pectoral species №4 was carried out using the atomic emission spectrometry. It was found out that the content of macronutrients is in the range of 62,1-21705,63 mg/kg. Their average values decreased in the series: K> Ca > Mg > P > S > Na > Fe. Macronutrients appear in the amount of 10 to 80% in the infusion from the pectoral species № 4. The amount of potassium, sodium, magnesium, calcium, phosphorus, sulfur and iron in the pectoral species № 4 made 25-1100% of the daily requirement which allows to consider this species as an additional source of those macronutrients presence in the human body.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75145080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.52
A. Osipova, A. A. Pahotskaya
Microscopic research of the whole and crushed Alcea rosea leaves on twenty microslides according to the method described in the State Pharmacopoeia of the Republic of Belarus was carried out. Microscopic research was carried out comparing with microslides of the whole and crushed leaves of a closely related type of a medicinal plant, being officinal, Althaea officinalis. During microscopic research anatomical and diagnostic features of Alcea rosea leaves allowing to identify this plant in the whole or crushed form were discovered and studied. The main of them are the structure of epidermis, types of stomatal apparatus, structure of hairs, localization and types of calcium oxalate inclusions. Anatomical and diagnostic features of the whole and crushed leaves of Althaea officinalis allowing to conduct a comparative analysis with the leaves of Alcea rosea was revealed. The main features are the type and structure of the hairs, localization and types of calcium oxalate inclusions. The resulting complex of microdiagnostic signs has a broad significance for identification of plants of the Malvaceae family.
{"title":"MICROSCOPIC ANALYSIS OF ALCEA ROSEA LEAVES COMPARED TO ALTHAEA OFFICINALIS LEAVES","authors":"A. Osipova, A. A. Pahotskaya","doi":"10.52540/2074-9457.2021.4.52","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.52","url":null,"abstract":"Microscopic research of the whole and crushed Alcea rosea leaves on twenty microslides according to the method described in the State Pharmacopoeia of the Republic of Belarus was carried out. Microscopic research was carried out comparing with microslides of the whole and crushed leaves of a closely related type of a medicinal plant, being officinal, Althaea officinalis. During microscopic research anatomical and diagnostic features of Alcea rosea leaves allowing to identify this plant in the whole or crushed form were discovered and studied. The main of them are the structure of epidermis, types of stomatal apparatus, structure of hairs, localization and types of calcium oxalate inclusions. Anatomical and diagnostic features of the whole and crushed leaves of Althaea officinalis allowing to conduct a comparative analysis with the leaves of Alcea rosea was revealed. The main features are the type and structure of the hairs, localization and types of calcium oxalate inclusions. The resulting complex of microdiagnostic signs has a broad significance for identification of plants of the Malvaceae family.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"227 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77449245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.22
V. A. Malatok, S. E. Rzheussky
This article is devoted to the pharmaceutical development of medical hemostatic foam. The method of parenchymal bleeding modeling in the liver of rats was used to assess hemostatic activity of various concentrations of aluminum and iron chloride. Also assessment of sedimentation rate and density of various samples of medical foam was made when polysorbates-20 and -80 were added to it in concentration range of 0-0,75%. It was found that from the point of view of its safety and efficacy concentration of aluminum chloride 10%, without iron salt is most acceptable. After evaluating density and sedimentation rate polysorbate-20 in concentrations of 0,25-0,75% was chosen as an additive.
{"title":"PHARMACEUTICAL DEVELOPMENT OF MEDICAL HEMOSTATIC FOAM","authors":"V. A. Malatok, S. E. Rzheussky","doi":"10.52540/2074-9457.2021.4.22","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.22","url":null,"abstract":"This article is devoted to the pharmaceutical development of medical hemostatic foam. The method of parenchymal bleeding modeling in the liver of rats was used to assess hemostatic activity of various concentrations of aluminum and iron chloride. Also assessment of sedimentation rate and density of various samples of medical foam was made when polysorbates-20 and -80 were added to it in concentration range of 0-0,75%. It was found that from the point of view of its safety and efficacy concentration of aluminum chloride 10%, without iron salt is most acceptable. After evaluating density and sedimentation rate polysorbate-20 in concentrations of 0,25-0,75% was chosen as an additive.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81842489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.85
I. A. Savkov, O. M. Khishova
The paper presents pharmaceutical development of the composition and technology for producing medicines based on a red raspberry leaves dry extract. When developing the composition and technology of tablets and hard capsules based on a red raspberry leaves dry extract it was proposed to produce tableted and powder mass using wet granulation and direct compression. To develop the composition of tablets and hard capsules using wet granulation the method of mathematical planning of the experiment was used: Latin square 3x3. The influence of the following factors on the quality of the obtained tablets and the contents of hard capsules was studied: disintegrating agents, excipients, concentration of binder solution. Disintegration time and compressive strength of tablets were used as optimization parameters and flowability of the powder mass for hard capsules was also evaluated. Significant effect on tablets disintegration of factors A and B (the nature of the excipient and the disintegrant) was stated. Duncan's multiple rank test was used to propose preference series within these factors. Compressive strength of tablets was not influenced by any factors studied. Based on the studies carried out the composition of the tablets and the contents of hard capsules with the use of wet granulation was proposed. The development of the composition and technology for obtaining tablets and the contents of hard capsules by direct compression and without wetting and granulation, respectively was made. It is proposed to use microcrystalline cellulose, lactose monohydrate and silicon oxide as additives to make direct compression which will improve technological characteristics of the tablet and powder mass and make it possible to produce tablets and the contents of hard capsules by direct compression and without wetting and granulation. This, in turn, will contribute to an increase in the microbiological purity of the tablets and the contents of hard capsules and increase labor productivity during their production. To increase the stability of tablets based on dry extract of red raspberry leaves water-soluble film coating is proposed.
{"title":"PHARMACEUTICAL DEVELOPMENT OF THE COMPOSITION AND TECHNOLOGY FOR PRODUCING MEDICINES BASED ON RED RASPBERRY LEAVES DRY EXTRACT","authors":"I. A. Savkov, O. M. Khishova","doi":"10.52540/2074-9457.2021.4.85","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.85","url":null,"abstract":"The paper presents pharmaceutical development of the composition and technology for producing medicines based on a red raspberry leaves dry extract. When developing the composition and technology of tablets and hard capsules based on a red raspberry leaves dry extract it was proposed to produce tableted and powder mass using wet granulation and direct compression. To develop the composition of tablets and hard capsules using wet granulation the method of mathematical planning of the experiment was used: Latin square 3x3. The influence of the following factors on the quality of the obtained tablets and the contents of hard capsules was studied: disintegrating agents, excipients, concentration of binder solution. Disintegration time and compressive strength of tablets were used as optimization parameters and flowability of the powder mass for hard capsules was also evaluated. Significant effect on tablets disintegration of factors A and B (the nature of the excipient and the disintegrant) was stated. Duncan's multiple rank test was used to propose preference series within these factors. Compressive strength of tablets was not influenced by any factors studied. Based on the studies carried out the composition of the tablets and the contents of hard capsules with the use of wet granulation was proposed. The development of the composition and technology for obtaining tablets and the contents of hard capsules by direct compression and without wetting and granulation, respectively was made. It is proposed to use microcrystalline cellulose, lactose monohydrate and silicon oxide as additives to make direct compression which will improve technological characteristics of the tablet and powder mass and make it possible to produce tablets and the contents of hard capsules by direct compression and without wetting and granulation. This, in turn, will contribute to an increase in the microbiological purity of the tablets and the contents of hard capsules and increase labor productivity during their production. To increase the stability of tablets based on dry extract of red raspberry leaves water-soluble film coating is proposed.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82697891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.58
O. V. Mushkina
Annual renewable resource potential of medicinal plant materials in the Republic of Belarus - Black alder leaves and Gray alder leaves - has been studied. It was found that average annual supply of black alder leaves for the last 6 years was 7845898,5 tons and gray alder leaves - 733257,5 tons. The stock of black alder leaves is almost 10 times as high as the stock of gray alder leaves. During storing black alder wood from 2015 to 2019, on the average 65,623,8 tons of dry leaves were unused by-product. Annually sylvulas of black alder are created in the forest fund of the Ministry of Forestry of the Republic of Belarus, for the period from 2015 to 2020 1828 hectares were created. 10,92 ± 0,27 tons of dry black alder leaves and 4,00 + 0,04 tons of dry gray alder leaves cab be stored annually on 1 hectare on the average on the territory of the Republic of Belarus. The percentage of the yield of air-dry raw materials for black alder leaves - 37% ± 1% and for gray alder leaves - 48% ± 2% is experimentally stated.
{"title":"RESOURCE POTENTIAL OF BLACK ALDER AND GRAY ALDER LEAVES","authors":"O. V. Mushkina","doi":"10.52540/2074-9457.2021.4.58","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.58","url":null,"abstract":"Annual renewable resource potential of medicinal plant materials in the Republic of Belarus - Black alder leaves and Gray alder leaves - has been studied. It was found that average annual supply of black alder leaves for the last 6 years was 7845898,5 tons and gray alder leaves - 733257,5 tons. The stock of black alder leaves is almost 10 times as high as the stock of gray alder leaves. During storing black alder wood from 2015 to 2019, on the average 65,623,8 tons of dry leaves were unused by-product. Annually sylvulas of black alder are created in the forest fund of the Ministry of Forestry of the Republic of Belarus, for the period from 2015 to 2020 1828 hectares were created. 10,92 ± 0,27 tons of dry black alder leaves and 4,00 + 0,04 tons of dry gray alder leaves cab be stored annually on 1 hectare on the average on the territory of the Republic of Belarus. The percentage of the yield of air-dry raw materials for black alder leaves - 37% ± 1% and for gray alder leaves - 48% ± 2% is experimentally stated.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81711020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.32
A. A. Rykhlova, M. Pivovar
The article discusses the influence of various factors on procaine hydrochloride electromigration under conditions of electrophoretic separation by capillary electrophoresis. The rate of voltage rise in the capillary electrophoresis system has been studied and a device making it possible to measure the potential in the system independent of the program software has been developed. The influence of the difference in hydrostatic pressure at the inlet and outlet of the quartz capillary and the difference in the approach to the analysis when changing the base electrolyte are investigated. The influence of the sample injected zone length on reproducibility of assay results of procaine hydrochloride has been studied. In order to improve separation efficiency and reproducibility of quantitative analysis results it is recommended to replace the base electrolyte after each analysis, as well as to inject the sample avoiding short injection values and high pressure. The values of apparent mobility of the test substance which can be used in the development of methods for procaine hydrochloride assay by capillary electrophoresis are calculated. The research results obtained can be applied in chemical and pharmaceutical laboratories as well as in scientific research.
{"title":"FACTORS EFFECTING PROCAINE HYDROCHLORIDE ELECTROMIGRATION UNDER CONDITIONS OF CAPILLARY ELECTROPHORESIS","authors":"A. A. Rykhlova, M. Pivovar","doi":"10.52540/2074-9457.2021.4.32","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.32","url":null,"abstract":"The article discusses the influence of various factors on procaine hydrochloride electromigration under conditions of electrophoretic separation by capillary electrophoresis. The rate of voltage rise in the capillary electrophoresis system has been studied and a device making it possible to measure the potential in the system independent of the program software has been developed. The influence of the difference in hydrostatic pressure at the inlet and outlet of the quartz capillary and the difference in the approach to the analysis when changing the base electrolyte are investigated. The influence of the sample injected zone length on reproducibility of assay results of procaine hydrochloride has been studied. In order to improve separation efficiency and reproducibility of quantitative analysis results it is recommended to replace the base electrolyte after each analysis, as well as to inject the sample avoiding short injection values and high pressure. The values of apparent mobility of the test substance which can be used in the development of methods for procaine hydrochloride assay by capillary electrophoresis are calculated. The research results obtained can be applied in chemical and pharmaceutical laboratories as well as in scientific research.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79070101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52540/2074-9457.2021.4.93
R. V. Krauchanka, S. E. Rzheussky
The purpose of this article was to study pharmacological activity of wax, propolis and oleoresin aqueous extracts and gels based on them obtained in the conditions of the laboratory. Minimum suppressive and minimum bactericidal concentrations of wax, propolis and oleoresin aqueous extracts are obtained by a two-fold dilution method and their combined action with chlorhexidine bigluconate by the “checkerboard method” were studied. Antimicrobial and local anti-inflammatory activity of gels based on them was also studied. It was found that wax and oleoresin aqueous extracts have a weak antimicrobial effect against gram-positive and gram-negative microorganisms. Gels based on propolis and wax have a weak antimicrobial effect in concentration of 5%, gels based on oleoresin have a weak antimicrobial activity in concentration of 1% and an average antimicrobial activity in concentration of 5%. Combinations of aqueous extracts with chlorhexidine bigluconate have neither synergetic nor additive effect, i.e. combination of substances has the same effect on microorganisms as the substances separately. High anti-inflammatory activity of gels based on an aqueous extract of propolis was established.
{"title":"PHARMACOLOGICAL ACTIVITY OF AQUEOUS EXTRACTS OF MULTICOMPONENT PRODUCTS OF NATURAL ORIGIN AND GELS BASED ON THEM","authors":"R. V. Krauchanka, S. E. Rzheussky","doi":"10.52540/2074-9457.2021.4.93","DOIUrl":"https://doi.org/10.52540/2074-9457.2021.4.93","url":null,"abstract":"The purpose of this article was to study pharmacological activity of wax, propolis and oleoresin aqueous extracts and gels based on them obtained in the conditions of the laboratory. Minimum suppressive and minimum bactericidal concentrations of wax, propolis and oleoresin aqueous extracts are obtained by a two-fold dilution method and their combined action with chlorhexidine bigluconate by the “checkerboard method” were studied. Antimicrobial and local anti-inflammatory activity of gels based on them was also studied. It was found that wax and oleoresin aqueous extracts have a weak antimicrobial effect against gram-positive and gram-negative microorganisms. Gels based on propolis and wax have a weak antimicrobial effect in concentration of 5%, gels based on oleoresin have a weak antimicrobial activity in concentration of 1% and an average antimicrobial activity in concentration of 5%. Combinations of aqueous extracts with chlorhexidine bigluconate have neither synergetic nor additive effect, i.e. combination of substances has the same effect on microorganisms as the substances separately. High anti-inflammatory activity of gels based on an aqueous extract of propolis was established.","PeriodicalId":23657,"journal":{"name":"Vestnik Farmacii","volume":"194 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74513757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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