Liying Jiang, Xianbo Luo, Qing Tian, Hui Wang, Xinxia Cai
{"title":"Disposable Biosensor for Hemoglobin Determination in Whole Blood","authors":"Liying Jiang, Xianbo Luo, Qing Tian, Hui Wang, Xinxia Cai","doi":"10.1109/NEMS.2006.334687","DOIUrl":null,"url":null,"abstract":"A disposable biosensor for the determination of hemoglobin in whole blood is described. The sensing method is based on amperometric detection technology. Platinized thin-film gold electrode is employed as a base electrode for the immobilization of mediator potassium ferricyanide. Saponin hemolyses the erythrocytes and hemoglobin is released. The biosensor has characters of rapid response time (60 s), high accuracy (> 90%), small sample volume (3 muL) and good precision (CV = 3.6%). The biosensor connected with the homemade portable meter can be used to determine hemoglobin concentration in clinical diagnosis and home-care purpose for athletes and patients potentially","PeriodicalId":6362,"journal":{"name":"2006 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems","volume":"147 1","pages":"204-208"},"PeriodicalIF":0.0000,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2006 1st IEEE International Conference on Nano/Micro Engineered and Molecular Systems","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/NEMS.2006.334687","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
A disposable biosensor for the determination of hemoglobin in whole blood is described. The sensing method is based on amperometric detection technology. Platinized thin-film gold electrode is employed as a base electrode for the immobilization of mediator potassium ferricyanide. Saponin hemolyses the erythrocytes and hemoglobin is released. The biosensor has characters of rapid response time (60 s), high accuracy (> 90%), small sample volume (3 muL) and good precision (CV = 3.6%). The biosensor connected with the homemade portable meter can be used to determine hemoglobin concentration in clinical diagnosis and home-care purpose for athletes and patients potentially
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