Health quality and reduction of pathogenic transmission in tomato seeds using plant extracts

Mônica Shirley Brasil dos Santos e Silva, A. A. Rodrigues, E. K. C. Silva, Anna Christina Sanazário de Oliveira, Leonardo de Jesus Machado Gois de Oliveira, Natalia de Jesus Ferreira Costa, Maria Rosângela Malheiros Silva, R. Lemos
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引用次数: 3

Abstract

The objective of the present study was to assess the seed health quality, quantification of seed-seedling pathogen transmission and the effect of plant extracts in reducing plant pathogens in the seeds of the tomato varieties San Marzano and Ipa 6. For the seed health, the samples were disinfested, plated and assessed after seven days, according to the Brazilian Seed Analysis Rule. For the transmission rate, 12 trays were prepared with 100 seeds each and assessed at 7, 14 and 21 d.a.s. (days after seeding) using 100 seedlings. The plant (main root, stalk and leaves) tissues were plated in PDA culture medium and assessed after seven days of incubation. Aqueous extracts were prepared from cinnamon, basil, neem and eucalyptus with 0.5% concentration and the seeds were immersed in each solution for 10 minutes. Then, they plated and assessed after seven days. The health test showed that biggest incidences of Aspergillus fumigatus (26 %) and Aspergillus flavus (26 %) were occurred in the seeds of the varieties Ipa 6 and San Marzano, respectively. The fungi A. flavus, A. fumigatus, A. niger, R. stolonifer and Curvularia sp. were detected in quantification of transmission in the seeds of the two tomato varieties. The treatment with basil extract resulted in the least fungus incidence in the transmission quantification of ‘San Marzano’ tomato seeds, while on Ipa 6 seeds the eucalyptus treatment performed better. The interference of treatments was not observed in tomato seed germination. However, there was decrease in incident of pathogens in seeds treated with the plant extracts and different effects was observed based on type and species of the pathogen.
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利用植物提取物提高番茄种子的健康质量和减少病原传播
以番茄品种圣马扎诺(San Marzano)和伊帕6 (Ipa 6)为研究对象,对其种子健康质量、种子-幼苗病原菌传播定量及植物提取物对种子病原菌的抑制作用进行了研究。为了种子的健康,根据巴西种子分析规则,在7天后对样品进行消毒、电镀和评估。为测定传代率,制作12个托盘,每个托盘100粒种子,分别在播种后7、14和21 d (d后)使用100株幼苗进行评估。将植株(主根、茎和叶)组织镀于PDA培养基中,培养7天后进行评估。肉桂、罗勒、楝树和桉树分别以0.5%的浓度制备水提取物,将种子浸泡在每种溶液中10分钟。然后,他们在7天后进行电镀和评估。卫生试验结果表明,ipa6和San Marzano品种种子中烟曲霉(26%)和黄曲霉(26%)的发病率最高。在两个番茄品种的种子中定量检测到黄霉、烟霉、黑霉、匍匐茎霉和曲霉霉的传播。罗勒提取物处理在“圣马扎诺”番茄种子传播量化中的真菌发病率最低,而桉树处理在Ipa 6种子传播量化中的效果更好。处理对番茄种子萌发无干扰。然而,植物提取物处理的种子中病原菌的发生率有所下降,并且根据病原菌的类型和种类观察到不同的效果。
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