Evaluation of tenofovir disoproxil fumarate loaded silver nanoparticle on testicular morphology in experimental type-2 diabetic rats

IF 4.5 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Artificial Cells, Nanomedicine, and Biotechnology Pub Date : 2022-03-27 DOI:10.1080/21691401.2022.2042009

S. O. Olojede, S. Lawal, A. Dare, E. C. Naidu, C. Rennie, O. Azu

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引用次数: 3

Abstract

Abstract Reproductive derangement and metabolic disorders in human immunodeficiency virus (HIV) infected persons require a nanoparticle delivery system to convey antiretroviral drugs to the anatomical sanctuary such as testis. This study investigated the effects of tenofovir disoproxil fumarate (TDF) loaded silver nanoparticles (AgNPs) on the testicular oxidative stress, inflammatory cytokines and histology in male diabetic rats. Thirty-six Sprague-Dawley rats weighing 230 ± 20 g were randomly divided into diabetic and non-diabetic groups (n = 18). Diabetes was induced using the fructose-streptozotocin (Frt-STZ) rat model. Both groups were further divided into three (n = 6) and administered distilled water, TDF, or TDF-AgNP. Results obtained with the TDF-AgNP administration showed a significant increase (p < .05) in the reduced glutathione and catalase levels. Tumour necrosis factor-alpha and interleukin 6 were reduced in diabetic rats administered TDF-AgNP. More so, administration of TDF-AgNP to diabetic rats improved testicular histoarchitecture in diabetic rats. In addition, diabetic rats administered TDF-AgNP showed a significant reduction (p < .05) in blood glucose levels. TDF-AgNP to diabetic rats enhanced testicular antioxidant enzyme, reduced testicular inflammation, and alleviated structural derangements in the testis. Thus, the application of AgNP to deliver TDF may alleviate testicular toxicity and subsequently cater for neglected reproductive dysfunction during the management of HIV infection.

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富马酸替诺福韦二氧吡酯纳米银对实验性2型糖尿病大鼠睾丸形态的影响

人类免疫缺陷病毒(HIV)感染者的生殖紊乱和代谢紊乱需要纳米颗粒输送系统将抗逆转录病毒药物输送到睾丸等解剖庇护所。本研究探讨富马酸替诺福韦二氧proxil (TDF)负载银纳米颗粒(AgNPs)对雄性糖尿病大鼠睾丸氧化应激、炎症细胞因子和组织学的影响。选取体重230±20 g的sd大鼠36只，随机分为糖尿病组和非糖尿病组(n = 18)。采用果糖-链脲佐菌素(first - stz)大鼠模型诱导糖尿病。两组进一步分为3组(n = 6)，分别给予蒸馏水、TDF或TDF- agnp。结果显示，TDF-AgNP组的谷胱甘肽和过氧化氢酶水平显著升高(p < 0.05)。在给予TDF-AgNP的糖尿病大鼠中，肿瘤坏死因子- α和白细胞介素6降低。此外，糖尿病大鼠给予TDF-AgNP改善了糖尿病大鼠睾丸组织结构。此外，给予TDF-AgNP的糖尿病大鼠血糖水平显著降低(p < 0.05)。TDF-AgNP对糖尿病大鼠具有增强睾丸抗氧化酶、减轻睾丸炎症、减轻睾丸结构紊乱的作用。因此，应用AgNP递送TDF可能减轻睾丸毒性，并随后满足HIV感染管理期间被忽视的生殖功能障碍。

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来源期刊

Artificial Cells, Nanomedicine, and Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

10.90

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Artificial Cells, Nanomedicine and Biotechnology covers the frontiers of interdisciplinary research and application, combining artificial cells, nanotechnology, nanobiotechnology, biotechnology, molecular biology, bioencapsulation, novel carriers, stem cells and tissue engineering. Emphasis is on basic research, applied research, and clinical and industrial applications of the following topics:artificial cellsblood substitutes and oxygen therapeuticsnanotechnology, nanobiotecnology, nanomedicinetissue engineeringstem cellsbioencapsulationmicroencapsulation and nanoencapsulationmicroparticles and nanoparticlesliposomescell therapy and gene therapyenzyme therapydrug delivery systemsbiodegradable and biocompatible polymers for scaffolds and carriersbiosensorsimmobilized enzymes and their usesother biotechnological and nanobiotechnological approachesRapid progress in modern research cannot be carried out in isolation and is based on the combined use of the different novel approaches. The interdisciplinary research involving novel approaches, as discussed above, has revolutionized this field resulting in rapid developments. This journal serves to bring these different, modern and futuristic approaches together for the academic, clinical and industrial communities to allow for even greater developments of this highly interdisciplinary area.