Strain Development of Aspergillus brasiliensis Using Physical and Chemicals Mutagenesis for Possible Overproduction of Xylanase, Amylase, Protease and Cellulase under Submerged Fermentation (SmF)

Abstract

Aims: Xylanase is commonly involved in the complete hydrolysis of xylan which consisted of hemicelluloses the enhancement in amylase activity was also observed in two mutants after UV exposure for 25 minutes and EMS treatment for 60 minutes with the production of 0.481±0.007 U/mL and 0.304±0.040 U/mL compared to wild type with 0.232±0.021 U/mL, respectively. Notably, there were increment of 107% and 31% in activity in mutants compared to wild type. Nevertheless, no possible overproduction of protease and cellulase was detected after the physical and chemical mutagenesis in this study. Conclusion: In a nutshell, the mutants of A. brasiliensis with enhanced production of xylanase and amylase were anticipated to fulfill the industrial demand in more economical approach using agro-residual waste of wheat bran under SmF.